Chst10 (NM_145142) Mouse Tagged ORF Clone

CAT#: MG217755

Chst10 (tGFP-tagged) - Mouse carbohydrate sulfotransferase 10 (Chst10), (10ug)

Product Images

Circular map for MG217755

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chst10
• Synonyms: AI507003; AU041319; Hnk-1st; ST
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217755 representing NM_145142
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAATGTGAACCCATCAGGAAGGATCATCGAATGGCCCCTGTCTGACAACATGCACCATCAGTGGC
  TCCTGCTGGCTGCATGCTTTTGGGTGATTTTCATGTTCATGGTGGCCAGCAAGTTCATCACGTTGACCTT
  TAAGGATCCGGATGGGTATAGTGCCAAACAGGAGTTTGTGTTCCTGACGACCATGCCGGAAGCAGAGAAG
  CTAAGAGGAGAGAAGCATTTTCCTGAAGTCCCGAAGCCAACTGGGAAGATGCTTTCGGACAGCCGTCCTG
  ATCAGCCCCCGGTTTATCTGGAGCGGCTGGAGCTCATCAGAAACACCTGCAAGGAGGAGGCTCTGCGGAA
  CCTCTCACACACCGAGGTCTCGAAGTTCGTCCTGGATCGAATATTTGTCTGTGACAAGCACAAGATTCTT
  TTCTGTCAGACTCCCAAGGTGGGCAACACCCAGTGGAAGAAAGTGCTGATCGTCCTAAATGGAGCATTTT
  CTTCCATTGAAGAGATTCCTGAAAATGTAGTCCATGACCATGAGAAAAATGGCCTTCCACGCCTCTCTTC
  CTTCAGCAAAATAGGAATTCAGAAGCGATTGAAAACATACTTCAAGTTTTTTATTGTGAGAGATCCCTTT
  GAAAGACTGATTTCTGCCTTTAAGGATAAGTTTGTTCACAATCCTCGATTCGAGCCTTGGTACAGGCATG
  AGATAGCCCCAGGCATTATTAGAAAGTACCGGAAGAACCGGACAGAGACCCGGGGGATCCAGTTTGAAGA
  TTTTGTGCGCTACCTGGGTGATCCAAACCGCAGGTGGTTAGACCTTCAGTTTGGGGACCATATCATCCAC
  TGGGTGACCTACGTTGAACTCTGTGCGCCCTGTGAGATCAAGTACAGTGTGGTCGGACACCATGAGACCC
  TGGAGGCAGATGCCCCGTACATCCTGAAAGAAGCTGGCATAGACCATCTGGTGTCATACCCCACCATCCC
  TCCGGGCATCACCATGTACAACAGAACCAAGGTAGAACAGTACTTCCTGGGCATCAGCAAACGAGACATC
  CGGCGTCTCTATGCCCGCTTTGAAGGGGACTTCAAGCTCTTTGGGTATCAGAAACCAGATTTCTTGCTAA
  AT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217755 representing NM_145142
  Red=Cloning site Green=Tags(s)
  
  MENVNPSGRIIEWPLSDNMHHQWLLLAACFWVIFMFMVASKFITLTFKDPDGYSAKQEFVFLTTMPEAEK
  LRGEKHFPEVPKPTGKMLSDSRPDQPPVYLERLELIRNTCKEEALRNLSHTEVSKFVLDRIFVCDKHKIL
  FCQTPKVGNTQWKKVLIVLNGAFSSIEEIPENVVHDHEKNGLPRLSSFSKIGIQKRLKTYFKFFIVRDPF
  ERLISAFKDKFVHNPRFEPWYRHEIAPGIIRKYRKNRTETRGIQFEDFVRYLGDPNRRWLDLQFGDHIIH
  WVTYVELCAPCEIKYSVVGHHETLEADAPYILKEAGIDHLVSYPTIPPGITMYNRTKVEQYFLGISKRDI
  RRLYARFEGDFKLFGYQKPDFLLN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145142
• ORF Size: 1122 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145142.2, NP_660124.2
• RefSeq Size: 3135 bp
• RefSeq ORF: 1125 bp
• Locus ID: 98388
• UniProt ID: Q6PGK7
• Cytogenetics: 1 B
• Gene Summary: Catalyzes the transfer of sulfate to position 3 of terminal glucuronic acid of both protein- and lipid-linked oligosaccharides. Participates in biosynthesis of HNK-1 carbohydrate structure, a sulfated glucuronyl-lactosaminyl residue carried by many neural recognition molecules, which is involved in cell interactions during ontogenetic development and in synaptic plasticity in the adult. May be indirectly involved in synapse plasticity of the hippocampus, via its role in HNK-1 biosynthesis.[UniProtKB/Swiss-Prot Function]