Krt33b (NM_013570) Mouse Tagged ORF Clone

CAT#: MG217680

Krt33b (tGFP-tagged) - Mouse keratin 33B (Krt33b), (10ug)

Product Images

Circular map for MG217680

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Krt33b
• Synonyms: Ha3; Ha4; Krt1-3; mHa3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217680 representing NM_013570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATACAACTGCTGCCTGCCCGCCATGAGCTGCCGCACCAGCTGCTCTTCCCGGCCCTGCGTGCCCC
  CCAGCTGCCATGGCTGCACCCTGCCCGGGGCCTGCAACATCCCCGCCAATGTGGGCAACTGCAATTGGTT
  CTGTGAGGGCTCCTTCAATGGCAATGAGAGGGAGACCATGCAGTTCCTGAATGACCGCCTGGCCTCCTAT
  ATGGAGAAGGTGAGGCAGCTGGAGAGAGAGAATGCAGAGCTGGAATGTAAGATCCAAGAGAGGAACCAGC
  AGCAGGACCCCCTGGTGTGTCCTGCCTACCAGGCCTACTTCAGGACCATTGAGGAGCTGCAGCAGAAGAT
  TCTCTGTGGCAAGTCTGAGAACGGCAGGCTGGTGGTGCAGATCGACAATGCCAAGCTGGCTGCTGATGAC
  TTCAGGACCAAGTATGAGTCAGAGCTGTCCCTGCGGCAGCTGGTGGAGTCAGACCTCAATGGCCTGCGCA
  GGATCCTGGACGAACTGACCCTCTGCAAGTCTGATCTGGAGGCACAGGTGGAGTCCCTGAGGCAGGAGCT
  GCTGTGTCTCAAGCAAAACCATGAGGAGGAAGTCAACACCCTGCGCTGCCAGCTTGGAGACCGCCTCAAC
  GTAGAGGTGGACGCTGCTCCCACCGTGGACCTGAACCGAGTGCTCAACGAGACCAGGTGTCAGTACGAGG
  CCCTGGTGGAAACCAACCGCCGGGAAGTGGAGGAATGGTTCACCACACAGACAGAGGAGCTGAACAAGCA
  GGTGGTGTCTAGCTCAGAGCAGCTGCAGTCCTGCCAGGCAGAGATCATCGAGCTGAGACGCACAGTCAAT
  GCCCTGGAGATCGAGCTGCAGGCCCAGCACAGCATGAGAAACTCTCTGGAGAATACCCTGGCAGAGAGTG
  AGGCTCGCTACAGCTCTCAGCTGTCCCAGGTGCAGTGCCTGATCACCAACGTGGAGTCCCAGCTTGGTGA
  GATCCGGGCTGACCTGGAGCGTCAGAACCAGGAGTACCAGGTGTTGCTGGATGTCAAGGCCCGGCTGGAG
  TGTGAGATCAACACGTATAGGGGCCTGCTGGAGAGCGAGGACTGCAAGCTGCCCTGCAACCCCTGTGCCA
  CCACCAATGCCTGTGACAAGCCCATTGGACCCTGTGTCACAAACCCTTGTGTCACACGCTCCCGATGTGG
  ACCCTGCAATTCCTTTGGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217680 representing NM_013570
  Red=Cloning site Green=Tags(s)
  
  MPYNCCLPAMSCRTSCSSRPCVPPSCHGCTLPGACNIPANVGNCNWFCEGSFNGNERETMQFLNDRLASY
  MEKVRQLERENAELECKIQERNQQQDPLVCPAYQAYFRTIEELQQKILCGKSENGRLVVQIDNAKLAADD
  FRTKYESELSLRQLVESDLNGLRRILDELTLCKSDLEAQVESLRQELLCLKQNHEEEVNTLRCQLGDRLN
  VEVDAAPTVDLNRVLNETRCQYEALVETNRREVEEWFTTQTEELNKQVVSSSEQLQSCQAEIIELRRTVN
  ALEIELQAQHSMRNSLENTLAESEARYSSQLSQVQCLITNVESQLGEIRADLERQNQEYQVLLDVKARLE
  CEINTYRGLLESEDCKLPCNPCATTNACDKPIGPCVTNPCVTRSRCGPCNSFGC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013570
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013570.1, NP_038598.1
• RefSeq Size: 1596 bp
• RefSeq ORF: 1215 bp
• Locus ID: 16671
• UniProt ID: Q61897
• Cytogenetics: 11 63.37 cM