Pstpip1 (NM_011193) Mouse Tagged ORF Clone

CAT#: MG217594

Pstpip1 (tGFP-tagged) - Mouse proline-serine-threonine phosphatase-interacting protein 1 (Pstpip1), (10ug)

Product Images

Circular map for MG217594

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pstpip1
• Synonyms: CD2BP1; def-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217594 representing NM_011193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCCCAGCTGCAGTTCCGAGATGCCTTCTGGTGCAGGGACTTCACGGCCCACACAGGGTATGAGG
  TGCTACTGCAGAGGCTGCTGGACGGCAGGAAGATGTGCAAGGATGTGGAGGAGCTGCTCAGACAGAGGGC
  CCAGGCGGAGGAGAGGTACGGGAAGGAGCTGGTGCAGATTGCACGCAAGGCTGGTGGCCAGACAGAGATG
  AATTCCCTGAGGACCTCCTTTGACTCCCTGAAGCAGCAAACAGAGAATGTGGGCAGTGCACACATCCAGC
  TGGCCCTGGCCCTGCGTGAGGAGCTGCGGAGCCTGGAGGAGTTCCGAGAGAGACAGAAAGAGCAGCGGAA
  GAAGTATGAGGCCATCATGGACCGTGTCCAGAAGAGCAAGTTGTCGCTCTACAAGAAGACCATGGAGTCC
  AAGAAGGCATATGACCAGAAGTGCAGGGATGCAGATGATGCTGAGCAGGCCTTCGAGCGTGTGAGTGCCA
  ATGGCCACCAGAAGCAAGTAGAAAAGAGCCAGAACAAAGCCAAGCAGTGCAAGGAGTCAGCCACAGAGGC
  AGAAAGAGTGTACAGGCAAAATATCGAACAACTGGAGAGAGCGAGGACCGAGTGGGAGCAGGAGCACCGG
  ACTACCTGTGAGGCCTTCCAGTTGCAGGAGTTTGACCGGCTCACCATCCTCCGCAACGCCCTGTGGGTGC
  ACTGTAACCAGCTCTCCATGCAGTGTGTCAAGGATGATGAGCTCTATGAGGAAGTGCGGCTGACCCTTGA
  GGGCTGTGATGTGGAAGGTGACATCAATGGCTTCATCCAGTCCAAGAGCACTGGCAGAGAGCCCCCAGCT
  CCGGTGCCTTATCAGAACTACTATGACAGGGAGGTGACCCCACTGATTGGCAGCCCTAGCGTCCAGCCCT
  CCTGTGGTGTGATAAAGAGGTTCTCTGGGCTGCTACATGGAAGTCCCAAGACCACACCTTCTGCTCCTGC
  TGCTTCCACAGAGACTCTGACTCCCACCCCTGAGCGGAATGAGTTGGTCTACGCATCCATCGAAGTGCAG
  GCGACCCAGGGAAACCTTAACTCATCAGCCCAGGACTACCGGGCACTCTACGACTACACCGCACAGAATT
  CTGATGAGCTGGACATTTCCGCGGGAGACATCCTGGCGGTCATCCTGGAAGGGGAGGATGGCTGGTGGAC
  TGTGGAGCGGAACGGACAACGTGGCTTTGTCCCTGGGTCGTACTTGGAGAAGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217594 representing NM_011193
  Red=Cloning site Green=Tags(s)
  
  MMAQLQFRDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDVEELLRQRAQAEERYGKELVQIARKAGGQTEM
  NSLRTSFDSLKQQTENVGSAHIQLALALREELRSLEEFRERQKEQRKKYEAIMDRVQKSKLSLYKKTMES
  KKAYDQKCRDADDAEQAFERVSANGHQKQVEKSQNKAKQCKESATEAERVYRQNIEQLERARTEWEQEHR
  TTCEAFQLQEFDRLTILRNALWVHCNQLSMQCVKDDELYEEVRLTLEGCDVEGDINGFIQSKSTGREPPA
  PVPYQNYYDREVTPLIGSPSVQPSCGVIKRFSGLLHGSPKTTPSAPAASTETLTPTPERNELVYASIEVQ
  ATQGNLNSSAQDYRALYDYTAQNSDELDISAGDILAVILEGEDGWWTVERNGQRGFVPGSYLEKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011193
• ORF Size: 1245 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011193.2, NP_035323.2
• RefSeq Size: 1853 bp
• RefSeq ORF: 1248 bp
• Locus ID: 19200
• UniProt ID: P97814
• Cytogenetics: 9 B
• Gene Summary: Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation. Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.[UniProtKB/Swiss-Prot Function]