Hnrnpm (NM_029804) Mouse Tagged ORF Clone

CAT#: MG217561

Hnrnpm (tGFP-tagged) - Mouse heterogeneous nuclear ribonucleoprotein M (Hnrnpm) transcript variant 1, (10ug)

Product Images

Circular map for MG217561

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hnrnpm
• Synonyms: 2610023M21Rik; AA409009; Hnrpm; mKIAA4193
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217561 representing NM_029804
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGGGGTCGAAGCGGCAGCCGAAGTGGCGGCGACAGAACCCAAAATGGAGGAAGAGAGCGGCG
  CGCCCTGCGTGCCGAGCGGCAACGGAGCTCCGGGCCCGAAGGGTGAAGAACGACCTACTCAGAATGAGAA
  GAGGAAGGAGAAAAACATAAAAAGAGGAGGCAATCGCTTTGAGCCATATTCCAACCCAACTAAAAGATAC
  AGAGCCTTCATTACAAATATACCTTTTGATGTGAAATGGCAGTCACTTAAAGACCTGGTTAAAGAAAAAG
  TTGGTGAGGTAACATACGTGGAGCTCTTAATGGACGCTGAAGGAAAGTCAAGGGGATGTGCTGTTGTTGA
  ATTCAAGATGGAGGAGAGCATGAAAAAAGCTGCTGAAGTTCTAAACAAGCATAGTCTGAGTGGAAGGCCA
  CTGAAAGTCAAGGAAGATCCTGATGGTGAACATGCAAGGAGAGCAATGCAAAAGGTGATGGCTACGACTG
  GTGGGATGGGTATGGGACCAGGTGGCCCAGGAATGATTAATATCCCACCCAGTATCCTAAATAATCCTAA
  CATCCCAAATGAGATTATCCATGCATTACAGGCTGGAAGACTTGGAAGCACAGTATTTGTAGCAAATCTG
  GATTATAAAGTTGGCTGGAAGAAACTGAAGGAAGTATTTAGTATGGCTGGTGTGGTGGTCCGAGCAGACA
  TTCTGGAAGATAAAGATGGGAAAAGTCGTGGAATAGGCATTGTGACTTTTGAACAGTCCATTGAAGCTGT
  GCAAGCAATATCTATGTTTAATGGCCAGTTGCTGTTTGATAGACCGATGCACGTCAAGATGGATGAGAGG
  GCTTTACCAAAGGGAGACTTTTTTCCTCCTGAACGCCCACAGCAACTTCCCCATGGACTTGGTGGTATTG
  GCATGGGATTAGGACCTGGAGGTCAGCCTATTGATGCCAACCATCTGAGCAAAGGCATTGGAATGGGAAA
  CCTAGGACCTGCAGGGATGGGAATGGAAGGCATAGGATTTGGAATAAATAAAATTGGAGGCATGGAAGGA
  CCCTTTGGTGGTGGTATGGAAAACATGGGCCGATTTGGATCCGGGATGAACATGGGCCGAATAAATGAAA
  TCCTAAGTAATGCACTGAAGAGAGGAGAGATCATTGCAAAACAGGGAGGAGGTGGAGCTGGAGGCAGTGT
  CCCTGGGATCGAGAGGATGGGCCCTGGCATTGACCGCATTAGCGGTGCTGGCATGGAGCGCATGGGCGCA
  GGCTTAGGCCATGGCATGGATCGAGTGGGCTCTGAGATTGAGCGCATGGGCCTGGTCATGGACCGCATGG
  GCTCAGTTGAGCGCATGGGCTCCAGCATTGAGCGCATGGGCCCACTAGGCCTTGACCACATGGCCTCCAG
  TATTGAGCGCATGGGCCAGACCATGGAGCGCATTGGTTCTGGCGTGGAGCGCATGGGTGCCGGCATGGGC
  TTCGGCCTGGAGCGCATGGCCGCACCCATTGACCGTGTGGGCCAAACCATTGAGCGCATGGGCTCTGGTG
  TAGAGCGCATGGGTCCTGCCATTGAACGCATGGGCCTAAGCATGGATCGGATGGTGCCCACAGGCATGGG
  GGCCAGCCTGGAGCGCATGGGTCCTGTGATGGATCGGATGGCCACCGGCCTGGAGCGCATGGGCGCCAAC
  AACCTGGAGCGCATGGGCCTGGAGCGTATGGGAGCCAACAGTCTTGAGCGCATGGGCCTGGAGCGAATGG
  GCGCCAACAGCCTGGAACGTATGGGCCCTGCCATGGGCCCAGCATTGGGCGCTGGTATTGAGCGAATGGG
  CCTGGCCATGGGTGGAGCCGGAGGTGCTAGCTTTGACCGAGCCATTGAGATGGAGCGGGGCAACTTTGGA
  GGAAGCTTCGCAGGTTCCTTTGGCGGAGCTGGAGGCCATGCACCTGGAGTAGCCAGGAAGGCCTGCCAGA
  TATTTGTGAGAAATCTCCCATTTGATTTTACATGGAAGATGCTAAAGGACAAGTTCAATGAATGTGGCCA
  CGTGCTGTACGCCGACATCAAGATGGAGAACGGAAAGTCCAAGGGGTGCGGTGTGGTTAAGTTTGAGTCT
  CCAGAGGTGGCTGAGAGAGCCTGCCGGATGATGAATGGCATGAAGCTGAGTGGCCGAGAGATTGATGTTC
  GAATTGATAGAAATGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217561 representing NM_029804
  Red=Cloning site Green=Tags(s)
  
  MAAGVEAAAEVAATEPKMEEESGAPCVPSGNGAPGPKGEERPTQNEKRKEKNIKRGGNRFEPYSNPTKRY
  RAFITNIPFDVKWQSLKDLVKEKVGEVTYVELLMDAEGKSRGCAVVEFKMEESMKKAAEVLNKHSLSGRP
  LKVKEDPDGEHARRAMQKVMATTGGMGMGPGGPGMINIPPSILNNPNIPNEIIHALQAGRLGSTVFVANL
  DYKVGWKKLKEVFSMAGVVVRADILEDKDGKSRGIGIVTFEQSIEAVQAISMFNGQLLFDRPMHVKMDER
  ALPKGDFFPPERPQQLPHGLGGIGMGLGPGGQPIDANHLSKGIGMGNLGPAGMGMEGIGFGINKIGGMEG
  PFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGGAGGSVPGIERMGPGIDRISGAGMERMGA
  GLGHGMDRVGSEIERMGLVMDRMGSVERMGSSIERMGPLGLDHMASSIERMGQTMERIGSGVERMGAGMG
  FGLERMAAPIDRVGQTIERMGSGVERMGPAIERMGLSMDRMVPTGMGASLERMGPVMDRMATGLERMGAN
  NLERMGLERMGANSLERMGLERMGANSLERMGPAMGPALGAGIERMGLAMGGAGGASFDRAIEMERGNFG
  GSFAGSFGGAGGHAPGVARKACQIFVRNLPFDFTWKMLKDKFNECGHVLYADIKMENGKSKGCGVVKFES
  PEVAERACRMMNGMKLSGREIDVRIDRNA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029804
• ORF Size: 2187 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029804.3, NP_084080.1
• RefSeq Size: 2532 bp
• RefSeq ORF: 2190 bp
• Locus ID: 76936
• UniProt ID: Q9D0E1
• Cytogenetics: 17 B1
• Gene Summary: Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines (By similarity).[UniProtKB/Swiss-Prot Function]