Enpp3 (NM_134005) Mouse Tagged ORF Clone

CAT#: MG217541

Enpp3 (tGFP-tagged) - Mouse ectonucleotide pyrophosphatase/phosphodiesterase 3 (Enpp3), (10ug)

Product Images

Circular map for MG217541

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Enpp3
• Synonyms: AI876438; CD203c
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217541 representing NM_134005
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCAGGCTAGCATTAGCCACAGAGGAGCCCATTAAGAAAGACAGTCTCAAGAAATACAAGATCC
  TTTGTGTGGTTCTTCTTGCTTTGTTGGTGATTGTATCGCTTGGATTGGGGCTGGGACTGGGACTCAGGAA
  ACCTGAAGAACAAGGCAGCTGCAGGAAAAAATGCTTTGACTCATCGCACAGAGGACTGGAGGGCTGCCGC
  TGTGACTCGGGATGCACAGGACGAGGAGACTGCTGCTGGGACTTTGAAGATACCTGTGTGAAATCAACTC
  AAATATGGACGTGCAATTTATTCCGTTGTGGGGAGAACAGACTGGAGACAGCCCTCTGTTCCTGTGCGGA
  TGACTGTCTACAGAGGAAGGACTGCTGTGCTGACTACAAGACTGTGTGCCAAGGCGAAAGCCCATGGGTG
  ACAGAAGCCTGTGCCTCATCCCAGGAGCCCCAGTGTCCACCAGGGTTTGACCTGCCACCAGTTATCTTGT
  TCTCCATGGACGGTTTTAGAGCTGAGTATTTACAAACATGGAGTACCCTTTTGCCTAATATCAACAAACT
  GAAAACATGTGGAATCCATTCTAAATATATGAGAGCTATGTATCCCACCAAAACCTTCCCAAATCATTAT
  ACCATTGTCACGGGTTTGTATCCGGAGTCACACGGCATCATTGACAACAACATGTATGACGTGCACCTCA
  ACAAGAATTTTTCCCTTTCTTCAGTGGAGAAAAGCAATCCCGCCTGGTGGAGCGGGCAGCCGATTTGGCT
  AACGGCAATGTATCAAGGTTTAAAAGCTGCTTGCTACTACTGGCCGGGATCAGATGTGGCTGTCAATGGC
  TCCTTCCCTACCATCTACAGGAATTACAGCAATTCTGTCCCATATGAAAGGAGGATTACAACACTGTTAC
  AATGGCTGGACCTGCCCAAAGCTGACCGACCCAGTTTTTATACCATCTATGTGGAAGAGCCTGATTCTGC
  AGGGCATTCGAGTGGACCAGTCAGTGCTGGAGTAATTAAAGCCTTGCAGTCAGTTGACAATGCCTTTGGA
  ATGTTGATGGAGGGTCTGAAACAGAGGAATTTACACAACTGTGTCAATATAATCGTTCTGGCTGATCATG
  GAATGGACCAGACCTCCTGTGATAGAGTGGAATACATGACAGATTATTTTCCTAAAATAAACTTCTATAT
  GTATCAAGGACCTGCTCCTCGAATCAGGACTCGTAATATACCTCAGGACTTCTTTACATTTAATTCTGAA
  GAGATTGTCAGGAACCTCAGTTGTCGAAAACCTGACCAACACTTTAAACCCTACTTGACCCCTGATTTGC
  CGAAACGACTGCACTACGCCAAAAATGTCAGAATTGACAAAGCTCATCTCATGGTGGATCGGCAATGGCT
  GGCTTTCAGGAGCAAAGGGAGTTCCAATTGTGGTGGAGGAACACATGGCTACAACAATGAGTTCAAGAGC
  ATGGAGGCAATCTTTTTGGCACATGGACCCAGCTTTATAGAGAAGACTGTTATTGAACCATTTGAAAATA
  TTGAAGTCTATAATCTACTGTGTGATCTTCTGCACATTGAACCAGCACCAAACAACGGTACTCATGGCAG
  TCTGAATCATCTTCTGAAGACACCGTTTTACAAGCCATCACATGCAGGAGAGTTGTCAACGCCTGCTGAC
  TGTGGTTTTACTACTCCCCTGCCTACAGACCCACTAGACTGCTCATGCCCTGCACTACAGAATACGCCTG
  GTCTAGAAGAGCAAGCAAACCAAAGGCTAAATCTTAGCGAAGGCGAGGTTGCAGCAACGGTGAAAGCAAA
  TTTGCCATTTGGGAGGCCCAGGGTGATGCAGAAGAACGGAGACCACTGTCTCCTTTACCACAGGGACTAC
  ATCAGTGGGTACGGCAAAGCTATGAAGATGCCCATGTGGAGTTCATACACCGTTCTCAAGCCGGGAGACA
  CATCATCTCTTCCTCCCACAGTTCCAGACTGTCTGCGGGCTGATGTCAGGGTTGCTCCTTCTGAGAGCCA
  AAAATGTTCCTTCTATTTAGCAGACAAGAATATCACCCATGGCTTCCTCTATCCTGCAATCAAAGGAACT
  AATGAGAGTCGATATGATGCCTTAATTACAAGTAATTTGGTCCCAATGTATAAAGAATTCAAAAAAATGT
  GGGACTACTTCCATGAGGTTCTTCTTATAAAATATGCTATAGAAAGAAATGGACTGAATGTGGTTAGTGG
  ACCCATCTTTGATTATAATTATGATGGCCATTTTGATGCTCCGGATGAAATCACACAATATGTAGCTGGC
  ACTGATGTCCCTATCCCAACACACTACTTTGTGGTGCTGACAAGTTGTAAAGACCAAACCCACACACCCG
  ACAGCTGCCCTGGGTGGCTGGATGTCCTGCCCTTCATCGTCCCTCACCGGCCCACCAACATAGAAAGCTG
  TTCTGAAAACAAGACCGAGGACCTTTGGGTTGAAGAGAGGTTCCAAGCACACGCCGCCCGGGTCCGAGAT
  GTGGAGCTCCTCACTGGCCTTGACTTTTATCAGGAGAAAGCACAGCCCGTCTCTCAGATTTTGCAACTAA
  AGACGTATCTGCCCACGTTTGAAACCATTATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217541 representing NM_134005
  Red=Cloning site Green=Tags(s)
  
  MDSRLALATEEPIKKDSLKKYKILCVVLLALLVIVSLGLGLGLGLRKPEEQGSCRKKCFDSSHRGLEGCR
  CDSGCTGRGDCCWDFEDTCVKSTQIWTCNLFRCGENRLETALCSCADDCLQRKDCCADYKTVCQGESPWV
  TEACASSQEPQCPPGFDLPPVILFSMDGFRAEYLQTWSTLLPNINKLKTCGIHSKYMRAMYPTKTFPNHY
  TIVTGLYPESHGIIDNNMYDVHLNKNFSLSSVEKSNPAWWSGQPIWLTAMYQGLKAACYYWPGSDVAVNG
  SFPTIYRNYSNSVPYERRITTLLQWLDLPKADRPSFYTIYVEEPDSAGHSSGPVSAGVIKALQSVDNAFG
  MLMEGLKQRNLHNCVNIIVLADHGMDQTSCDRVEYMTDYFPKINFYMYQGPAPRIRTRNIPQDFFTFNSE
  EIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKAHLMVDRQWLAFRSKGSSNCGGGTHGYNNEFKS
  MEAIFLAHGPSFIEKTVIEPFENIEVYNLLCDLLHIEPAPNNGTHGSLNHLLKTPFYKPSHAGELSTPAD
  CGFTTPLPTDPLDCSCPALQNTPGLEEQANQRLNLSEGEVAATVKANLPFGRPRVMQKNGDHCLLYHRDY
  ISGYGKAMKMPMWSSYTVLKPGDTSSLPPTVPDCLRADVRVAPSESQKCSFYLADKNITHGFLYPAIKGT
  NESRYDALITSNLVPMYKEFKKMWDYFHEVLLIKYAIERNGLNVVSGPIFDYNYDGHFDAPDEITQYVAG
  TDVPIPTHYFVVLTSCKDQTHTPDSCPGWLDVLPFIVPHRPTNIESCSENKTEDLWVEERFQAHAARVRD
  VELLTGLDFYQEKAQPVSQILQLKTYLPTFETII
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134005
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134005.2, NP_598766.2
• RefSeq Size: 2815 bp
• RefSeq ORF: 2625 bp
• Locus ID: 209558
• UniProt ID: Q6DYE8
• Cytogenetics: 10 A4
• Gene Summary: Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (By similarity). Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines (PubMed:25692702). Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (PubMed:28225814). Has also alkaline phosphodiesterase activity (By similarity).[UniProtKB/Swiss-Prot Function]