Tars2 (NM_001163618) Mouse Tagged ORF Clone

CAT#: MG217503

Tars2 (tGFP-tagged) - Mouse threonyl-tRNA synthetase 2 mitochondrial (putative) (Tars2) nuclear gene encoding mitochondrial protein transcript variant 3, (10ug)

Product Images

Circular map for MG217503

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tars2
• Synonyms: 2610024N01Rik; AI429208; Tarsl1; thrRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217503 representing NM_001163618
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCTCTGTCTGAGGTGGCGCCGGCTTGGGTTCCCACTCCCAGAGTTCCGCCGCTGCGAGCTCCACA
  CCGTGCGTGAGGCCTCTGCACCAACTCCTCCACATTGGTTGGCAGAACGATTTGGCCTTTTTGAGGAGCT
  ATGGACCGCTCACGTGAAAAAGTTAGCAAGTATGACACAGAAGAAAGCCCGGGCTATTAAGATATCACTT
  CCTGAAGGCCAGAAGGTAGATGCTGTTGCATGGAACACAACCCCTTACCAACTGGCCCATCAGATCAGTG
  TAACACTGGCTGATACTGCAGTGGCTGCTGAAGTAAATGGAGAACTTTACGATCTGGACCGACCCTTGGA
  GACAGATTGTCACCTCAGATTTCTGACATTTGATTCCCCAGAGGGCAAAGCGGACAACCACTTTAAGCTT
  CATCTGATCGAGGAGAAAGTGACAGGCCCAACGGCAACAGTGTATGGGTGTGGCATGTCAGTTGACCTGT
  GCCGAGGCCCCCATCTTCGGCACACTGGACAGATTGGAGCACTGAAGCTGCTCACGAACTCCTCAGCCTT
  GTGGAGGTCCTTGGGAGCACCTGAGACACTGCAGAGGGTATCAGGAATTTCTTTCCCCAAAGTAGAGTTA
  CTGAGGAACTGGGAAGCTCGAAGAGAAGCAGCAGAGTTAAGAGACCACAGACGCATTGGGAAGGAACAGG
  AGCTCTTCTTCTTCCATGAACTGAGCCCTGGGAGCTGTTTTTTCTTGCCACGAGGGACAAGAGTCTATAA
  TGCCCTGGTGGCTTTCATCAGGGCTGAGTATGCCCGCCGTGGTTTCTCAGAGGTGAAAACTCCCACGCTG
  TTTTCTACAAAACTCTGGGAACAGTCAGGGCACTGGGAACACTATAGGGCAGACATGTTTTCCCTGAAGC
  CCCCTGGCACTGATGGTGTTGACAACTCCCAGAGTGGCCATCCTGCCAGGTGTCCCAAAGACACACTTGC
  TCTAAAGCCCATGAACTGCCCTGCACACTGCCTGATGTTTGCCCACCGGCCCAGATCCTGGAGGGAACTG
  CCTGTGCGACTGGCTGATTTCGGAGCCCTGCATCGGGCTGAGGCCTCTGGCAGTCTGGGAGGATTAACGC
  GGCTGTGGCGCTTCCAGCAGGATGATGCGCACATCTTTTGTGCACCCCATCAGCTGGAAGCAGAGATCCA
  GGGCTGCCTTGATTTTCTCCGGTGTGTTTACTCGGTTCTTGGTTTTTCCTTCCACCTGGCTTTATCTACC
  CGGCCACCTGGTTTCCTAGGGGAGCCTCGCCTATGGGACCAGGCTGAGCAGGTTCTACAGCAAGCCTTGG
  AGAAGTTTGGAGAACCTTGGGACCTCAACCCTGGAGATGGGGCTTTCTATGGGCCTAAGATTGATGTGCA
  CCTCCACGACGCCCTTGGTCGGCCCCATCAGTGTGGAACAATCCAGCTTGACTTCCAGCTGCCACTGAGA
  TTTGACCTACAATACAAGGGGCCGGCAGGTACCCCAGAGTGTCCAGTCCTTATTCATCGAGCAGTGCTTG
  GTTCTGTGGAAAGGCTGTTGGGAGTGCTGGCAGAAAGCTGTGGGGGGAAATGGCCCCTGTGGCTGTCCCC
  GCTCCAGGTGGTGGTCATCCCTGTGAGGACAGAGCAAGAGGAATATGCCAGGCAGGTGCAACAGTGCCTG
  CAGGCTGCAGGACTTGTGAGTGATCTCGATGCAGACTCTGGACTGACCCTCAGCCGGAGAGTCCGCCGGG
  CCCAGCTTGCCCACTACAACTTTCAGTTTGTGGTTGGCCAGAGAGAGCAGAGTCAAAGGACAGTGAACGT
  ACGCACGCGAGATAACCGGCAGCTCGGGGAGCGAGACCTGGCCGAGTCCGTGCAGAGACTGCTGGAGTTA
  CAGAACGCCAGGGTCCCCAATGCAGAAGAAGTGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217503 representing NM_001163618
  Red=Cloning site Green=Tags(s)
  
  MGLCLRWRRLGFPLPEFRRCELHTVREASAPTPPHWLAERFGLFEELWTAHVKKLASMTQKKARAIKISL
  PEGQKVDAVAWNTTPYQLAHQISVTLADTAVAAEVNGELYDLDRPLETDCHLRFLTFDSPEGKADNHFKL
  HLIEEKVTGPTATVYGCGMSVDLCRGPHLRHTGQIGALKLLTNSSALWRSLGAPETLQRVSGISFPKVEL
  LRNWEARREAAELRDHRRIGKEQELFFFHELSPGSCFFLPRGTRVYNALVAFIRAEYARRGFSEVKTPTL
  FSTKLWEQSGHWEHYRADMFSLKPPGTDGVDNSQSGHPARCPKDTLALKPMNCPAHCLMFAHRPRSWREL
  PVRLADFGALHRAEASGSLGGLTRLWRFQQDDAHIFCAPHQLEAEIQGCLDFLRCVYSVLGFSFHLALST
  RPPGFLGEPRLWDQAEQVLQQALEKFGEPWDLNPGDGAFYGPKIDVHLHDALGRPHQCGTIQLDFQLPLR
  FDLQYKGPAGTPECPVLIHRAVLGSVERLLGVLAESCGGKWPLWLSPLQVVVIPVRTEQEEYARQVQQCL
  QAAGLVSDLDADSGLTLSRRVRRAQLAHYNFQFVVGQREQSQRTVNVRTRDNRQLGERDLAESVQRLLEL
  QNARVPNAEEVF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163618
• ORF Size: 1926 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163618.1, NP_001157090.1
• RefSeq Size: 2281 bp
• RefSeq ORF: 1929 bp
• Locus ID: 71807
• UniProt ID: Q3UQ84
• Cytogenetics: 3 F2.1
• Gene Summary: Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction: threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr). Also edits incorrectly charged tRNA(Thr) via its editing domain.[UniProtKB/Swiss-Prot Function]