Grpel2 (NM_021296) Mouse Tagged ORF Clone

CAT#: MG217444

Grpel2 (tGFP-tagged) - Mouse GrpE-like 2 mitochondrial (Grpel2) nuclear gene encoding mitochondrial protein, (10ug)

Product Images

Circular map for MG217444

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grpel2
• Synonyms: mt-GrpE#2; mt-Grpel2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217444 representing NM_021296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCGTTCGCTGTGGGCGGTGCAGCGGCTGCAGCGCCTCCTGGCCTCGGGGGCTATGTCAGAGA
  GCAGGGGATGGCTCCACCCATTCAGCACTGCCACCCAAAGAACTGCTGGGGAAGACTGCAGTTCCGAGGA
  CCCTCCTGATGGACTGGGACCCTCCCTTGCTGAGCAGGCCTTAAGGCTCAAAGCTGTTAAACTGGAAAAG
  GAAGTCCAGGATTTAACCCTGAGATACCAGAGAGCTGTCGCTGATTGTGAAAACATAAGGAGAAGAACCC
  AGAGATGTGTGGAAGACGCGAAGATATTTGGGATCCAGAGCTTCTGCAAGGACCTGGTGGAGGTGGCAGA
  CATTTTGGAGAAGACTGCCAAGTGCTGTTCAGAAGGGGCAGAACCTGAGGACCACAGGCGCACTCTGGAA
  AAAGTCTTCCAAGGGCTGTCCCTGCTAGAAGCCAGACTGAAAAGCGTATTTACCAAGCATGGCCTAGAGA
  AAATGACACCCATCGGTGACAAATACGACCCTCACGAGCATGAACTCATCTGTCACATGCCAGCAGGTGT
  TGGGGTACAGCCTGGCACCGTGGCATTAGTTCGGCAAGATGGCTACAAACTTCATGGCCGCACCATTAGA
  CTTGCGCAAGTGGAAGTGGCAGTAGAGTCTCAGAGAAGACTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217444 representing NM_021296
  Red=Cloning site Green=Tags(s)
  
  MAARSLWAVQRLQRLLASGAMSESRGWLHPFSTATQRTAGEDCSSEDPPDGLGPSLAEQALRLKAVKLEK
  EVQDLTLRYQRAVADCENIRRRTQRCVEDAKIFGIQSFCKDLVEVADILEKTAKCCSEGAEPEDHRRTLE
  KVFQGLSLLEARLKSVFTKHGLEKMTPIGDKYDPHEHELICHMPAGVGVQPGTVALVRQDGYKLHGRTIR
  LAQVEVAVESQRRL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021296
• ORF Size: 672 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021296.2, NP_067271.1
• RefSeq Size: 4065 bp
• RefSeq ORF: 675 bp
• Locus ID: 17714
• UniProt ID: O88396
• Cytogenetics: 18 E1
• Gene Summary: Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70.[UniProtKB/Swiss-Prot Function]