Tmco6 (NM_028036) Mouse Tagged ORF Clone

CAT#: MG217428

Tmco6 (tGFP-tagged) - Mouse transmembrane and coiled-coil domains 6 (Tmco6), (10ug)

Product Images

Circular map for MG217428

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmco6
• Synonyms: 2410015B03Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217428 representing NM_028036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAATCGGCGGCAGGGCCGCCTCAGGACGCTGGCGTTTGGGGTGGAGGAGCTGCGGCGCCGCCGGC
  GGGAGCGGGAGGCAGCCCTGCGGAAGGCGCGGAGGGAGCAGCAGTTAGTCAGCAAGAGGCTGCTGAGGGA
  AGACGCCCCGGAAGAAGTCGGAGGCCAGAGTGCAGCTGTGCTCCTTGGGGAAGCCGAGGTCCAGCAGTTC
  CTTCGGCTCGCCCAACGGGGGACAGATGAAAAGGAGAGGGAGAAGGCTCTGGTCAGCCTTCGTCGAGGCT
  TGCAGCACCCTGACACACAGCAAACCTTCATCAGGCTGGAGGGCAGCATGCGGACCCTTGTTGGGATTTT
  GACCAGCAACCGGGCCTTGTTGCAGCTTGAGGCGGCTCGGTGCCTTCATGAGCTCTCTCATTCTGAGCAG
  TCTGCAGTGGCTGAGGCCTGCCTGCCAGCGACTTCCTACCTCCTCACCTACCTCTCTGGTCACAGCTCAG
  ATTTCATAGAGCTGTGTCTGTACACACTGGGGAACCTCATCGTGGAGAGTGAGGCCGTGAGAAAGCAGCT
  CCTGCCGCAGGGCATTGTCCCAGCCTTTGCTGCCTGCATCCAGTCCCCCCATGTGGCTGTGCTGGAAGCA
  CTTGGATATGCCTTGTCCCAGCTTCTGCAGGCTAAGGAAGCCCCAGAGAAGATCATTCCCTCTATTCTGG
  ATTCCAGTCTTCCCCAGCAGATGCTACGGTTGATGCAACCAGGCCCGAAGCTGAACCTTGGGGTTGCCAT
  GGAGTTTGCTTGGTGCCTTCACTATATCATCTGCAGCCAGGTCAACAATGCTGTGCTCCTTACCCATGGG
  GCTCTGCCCACGTTGGCACTGCTGCTTTTGGACTTGGCTGGGACTGTGCAGAGAATGGATGATGTGGGAC
  TAGAGCTGCTTGCATGCCCCGTGCTTCGGTGTCTAAGCAACCTGTTAACAGAAGTGCCAGCGGAGGTCAT
  GGGACAGCAAATGGAGCTCAGAGACGAGCGACTTGTGGCAGCCTTATTTATCTTTCTCCAGTTCTTCCTT
  CAGAAACAGCCTGCCTTGCTACCTGAGGGCCTCTGGCTCCTCAACAACCTCACTGCAAACAGTCCTACTT
  TTTGTACCTCCTTGCTCTCTCTGGATCTGATCGAGCCGCTCTTGCAGTTGTTGCCACTATCAAATGCAGT
  ATGCATGTTGGTGCTCACAGTTCTGTGCAATGTCGTAGAGAAGGGTCCAGCTTACTGCCAGCGGTTGTGG
  CCAGGACCCCTGCTCTCCTGCGTGCTTAACACACTGGCTCTCTCTGACACCGAAGTAGTAGGCCAGAGTT
  TGGAGCTGCTGCAGCTGCTATTCTTGCATCAGCCAGAGGCTGCCCGAGCCTTCCTGCAGCAGTCAGGGCT
  GCAGGCCCTAGAAAAGCTCCAGGAGGAAACTCAGCTTCAGGAGAGGATACACGCGCTCCAGCAGATAGCT
  GCTACCCATGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217428 representing NM_028036
  Red=Cloning site Green=Tags(s)
  
  MWNRRQGRLRTLAFGVEELRRRRREREAALRKARREQQLVSKRLLREDAPEEVGGQSAAVLLGEAEVQQF
  LRLAQRGTDEKEREKALVSLRRGLQHPDTQQTFIRLEGSMRTLVGILTSNRALLQLEAARCLHELSHSEQ
  SAVAEACLPATSYLLTYLSGHSSDFIELCLYTLGNLIVESEAVRKQLLPQGIVPAFAACIQSPHVAVLEA
  LGYALSQLLQAKEAPEKIIPSILDSSLPQQMLRLMQPGPKLNLGVAMEFAWCLHYIICSQVNNAVLLTHG
  ALPTLALLLLDLAGTVQRMDDVGLELLACPVLRCLSNLLTEVPAEVMGQQMELRDERLVAALFIFLQFFL
  QKQPALLPEGLWLLNNLTANSPTFCTSLLSLDLIEPLLQLLPLSNAVCMLVLTVLCNVVEKGPAYCQRLW
  PGPLLSCVLNTLALSDTEVVGQSLELLQLLFLHQPEAARAFLQQSGLQALEKLQEETQLQERIHALQQIA
  ATHG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028036
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028036.3, NP_082312.3
• RefSeq Size: 1815 bp
• RefSeq ORF: 1485 bp
• Locus ID: 71983
• UniProt ID: Q8BQX5
• Cytogenetics: 18 B2