Fmo5 (NM_001161763) Mouse Tagged ORF Clone

CAT#: MG217144

Fmo5 (tGFP-tagged) - Mouse flavin containing monooxygenase 5 (Fmo5) transcript variant 2, (10ug)

Product Images

Circular map for MG217144

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fmo5
• Synonyms: 5033418D19Rik; AI195026
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217144 representing NM_001161763
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAAAAAAGGATTGCTGTAATTGGAGCAGGAGCTAGCGGGCTCACCTGCATCAAGTGCTGCCTGG
  AAGAGGGCTTGGAACCTGTCTGCTTTGAAAGGTCTGGTGACATCGGAGGACTGTGGAGATTCCAGGAAGC
  CCCTGAGGAAGGCAGGGCCAGTATTTACCAGTCCGTGGTCATCAACACCTCCAAGGAAATGATGTGTTTC
  AGTGACTACCCCATCCCAGATCATTACCCTAACTACATGCACAACTCCCAGGTCCTAGAGTATTTCAGGA
  TGTATGCCAAAGAATTTGACCTTCTCAAATATATCCAATTCAAGACTACAGTGTGCAGCGTGAAGAAGCA
  GCCTGATTTCTCTACCTCGGGCCAATGGCAGGTGGTCACTGAGTGTGAAGGGAAACAGCAGGTAGACGTC
  TTCGATGGAGTCCTGGTGTGCACGGGTCATCACACGGATGCTCACCTGCCCCTGGAAAGCTTTCCTGGAA
  TTGAAAAGTTCAAAGGAAAGTACTTTCACAGCCGAGACTATAAGAACCCAGTGGAATTCACCGGGAAGAG
  GGTCATTGTAATTGGCATTGGGAATTCTGGAGGGGATCTGGCTGTAGAGATTAGCCACACAGCCAAGCAG
  GTATTCCTCAGTACCAGGAGAGGAGCTTGGATCTTGAACCGTGTAGGCAAGCATGGATATCCTATTGACC
  TGCTACTCTCTTCTCGGATTATGTACTACTTGTCACGTATCTGTGGTCCATCACTAAAAAACAACTATAT
  GGAAAAACAGATGAACCAAAGGTTTGATCATGAAATGTTTGGCCTGAAACCTAAACACAGGGCTCTGAGT
  CAGCATCCAACAGTGAATGATGACCTGCCCAATCGTATCATTGCTGGCTTGGTGAAGGTGAAAGGAAATG
  TGAAGGAATTCACAGAGACAGCCGCCGTATTTGAGGATGGCTCCAGGGAGGATGGCATTGATGTGGTCAT
  CTTTGCCACAGGCTACAGCTTTGCCTTTCCTTTTCTTGAGGACTCTGTCAAAGTAGTAAAAAACAAGGTG
  TCCTTGTACAAAAAGGTCTTTCCACCTAACCTGGAAAAACCAACACTTGCAATCATCGGCTTAATTCAGC
  CCTTGGGAGCCATTATGCCCATTTCAGAGCTCCAAGGACGCTGGGCCACTCAAGTGTTCAAAGGGCTAAA
  GAAATTGCCCTCACAAAGTGAAATGATGGCAGAAATAAATAAGGCTCGAGAGGAAATGGCAAAAAGGTAT
  GTGGACAGCCAGCGTCATACCATCCAGGGAGACTATATAGACACCATGGAAGAGATTGCAGATTTAGTGG
  GTGTCAGGCCCAATATCCTGCCTCTGGTCTTCACTGACCCCAGGCTGGCATTACGGTTACTACTGGGGCC
  CTGTACTCCAGTCCAGTATCGCCTACAGGGCCCTGGAAAGTGGGCTGGAGCCCGGAAAACTATTCTCACC
  ACGGAAGATCGTGTTCGGAAGCCTCTGATGACAAGAGTGGTGGAAAGAGACAGCTCTGGGGGGTCACTGG
  TGACAGTGCGTGTCCTCATGCTGGCTGTTGCTTTCTTTGCTGTCATCCTGGCTTATTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217144 representing NM_001161763
  Red=Cloning site Green=Tags(s)
  
  MAKKRIAVIGAGASGLTCIKCCLEEGLEPVCFERSGDIGGLWRFQEAPEEGRASIYQSVVINTSKEMMCF
  SDYPIPDHYPNYMHNSQVLEYFRMYAKEFDLLKYIQFKTTVCSVKKQPDFSTSGQWQVVTECEGKQQVDV
  FDGVLVCTGHHTDAHLPLESFPGIEKFKGKYFHSRDYKNPVEFTGKRVIVIGIGNSGGDLAVEISHTAKQ
  VFLSTRRGAWILNRVGKHGYPIDLLLSSRIMYYLSRICGPSLKNNYMEKQMNQRFDHEMFGLKPKHRALS
  QHPTVNDDLPNRIIAGLVKVKGNVKEFTETAAVFEDGSREDGIDVVIFATGYSFAFPFLEDSVKVVKNKV
  SLYKKVFPPNLEKPTLAIIGLIQPLGAIMPISELQGRWATQVFKGLKKLPSQSEMMAEINKAREEMAKRY
  VDSQRHTIQGDYIDTMEEIADLVGVRPNILPLVFTDPRLALRLLLGPCTPVQYRLQGPGKWAGARKTILT
  TEDRVRKPLMTRVVERDSSGGSLVTVRVLMLAVAFFAVILAYF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161763
• ORF Size: 1599 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161763.1, NP_001155235.1
• RefSeq Size: 5202 bp
• RefSeq ORF: 1602 bp
• Locus ID: 14263
• UniProt ID: P97872
• Cytogenetics: 3 F2.2
• Gene Summary: In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme.[UniProtKB/Swiss-Prot Function]