Pex11b (NM_001162388) Mouse Tagged ORF Clone

CAT#: MG217097

Pex11b (tGFP-tagged) - Mouse peroxisomal biogenesis factor 11 beta (Pex11b) transcript variant 3, (10ug)

Product Images

Circular map for MG217097

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pex11b
• Synonyms: PEX11beta; Pex11pbeta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217097 representing NM_001162388
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAAACTGAGGGCCGCCCAGTATGCCTGTTCCCTTCTCGGCCATGCTTTGCAGAGACATGGGGCTA
  GTCCCGAGTTACAGAAACAGATTCGACAACTGGAGGGTCATCTGAGCCTCGGACGAAAGTTGCTACGCCT
  GGGGAACTCAACAGATGCCCTGGAGTCAGCCAAAAGGGCTGTGCACCTGTCAGATGTCGTTCTGAGATTC
  TGCATCACTGTCAGTCACCTCAACCGAGCCTTGTACTTTGCCTGTGACAATGTCCTATGGGCTGGAAAGT
  CTGGGCTAGCTCCCCGAGTGGACCAGGAAAAGTGGGCACAGCGTTCATTCAGATACTATCTGTTTTCCCT
  CATCATGAATCTGAGCCGTGATGCTTATGAGATTCGCCTATTGATGGAACAAGAGACTTCAGCTTACAGC
  AGGCGAATGAAGGTTTCTGGAGTAGGAGTCTCAGGAGGAGTAGAAACTGTGGGACCTGGAGGACCAGGGA
  CTCCAGGAGGAAGTCTGCCACAACTGGCTTTGAAGTTTCGGCTCCGAATCCTGCTACTGGCTCGTGTCCT
  TCGAGGTCATCCCCCTCTCTTGCTGGATGTGCTCAGAAATGCCTGTGACCTTTTCATCCCACTGGACAAA
  CTAGGCCTCTGGCGTTGTGGCCCTGGGATTGTGGGGCTGTGTGGCCTCATATCCTCCATTCTGTCTATTC
  TCACCCTAATCTGTCCCTGGCTACGACTCAAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217097 representing NM_001162388
  Red=Cloning site Green=Tags(s)
  
  MGKLRAAQYACSLLGHALQRHGASPELQKQIRQLEGHLSLGRKLLRLGNSTDALESAKRAVHLSDVVLRF
  CITVSHLNRALYFACDNVLWAGKSGLAPRVDQEKWAQRSFRYYLFSLIMNLSRDAYEIRLLMEQETSAYS
  RRMKVSGVGVSGGVETVGPGGPGTPGGSLPQLALKFRLRILLLARVLRGHPPLLLDVLRNACDLFIPLDK
  LGLWRCGPGIVGLCGLISSILSILTLICPWLRLKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162388
• ORF Size: 735 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162388.1, NP_001155860.1
• RefSeq Size: 837 bp
• RefSeq ORF: 738 bp
• Locus ID: 18632
• Cytogenetics: 3 F2.1
• Gene Summary: Involved in peroxisomal proliferation. May regulate peroxisome division by recruiting the dynamin-related GTPase DNM1L to the peroxisomal membrane. Promotes membrane protrusion and elongation on the peroxisomal surface.[UniProtKB/Swiss-Prot Function]