Efcab6 (NM_001161629) Mouse Tagged ORF Clone

CAT#: MG217033

Efcab6 (tGFP-tagged) - Mouse EF-hand calcium binding domain 6 (Efcab6) transcript variant 3, (10ug)

Product Images

Circular map for MG217033

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Efcab6
• Synonyms: 4931407K02Rik; 4932408N08Rik; bM150J22.2; mKIAA1672
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217033 representing NM_001161629
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGAAGAAACATGGATGTAAACAGAAAGTTGCAGATTCAGAGCTAGCCTGTGAGCAGGCTCATCAGT
  ACCTCATCATGAAAGCCAAAACCAGATGGGCAGACCTGTCCAAGAATTTCATAGAAACGGATAATGAGGG
  CAACGGCATCCTCCGGCGAAGGGACATCAAGAACTCGCTCTATGGCTTTGACATCCCCCTCACCCCAAGA
  GAATTTGAGAAGCTTTGGCAAAACTATGACACCGAGGGAAGGGGTTATATCACGTACCAAGAGTTCCTGC
  ACAGACTGGGCATTCGATACTCCCCGAAGGTCCACCGGCCCTACAAGGAAGACTACTTCAACTTCCTGGG
  TCACTTCACGAAGCCCAAGCAGGTCCAGGAGGAAATCCAGGAGCTACAGCAGATCAGTGAGAGGGAGAAA
  CTCATGAACCATTATGAGGAGATCAGCAAGGCGTTCAACGCAATGGAAAAATCCAAGCCCGTCGCCCTTT
  GCAGGGTGCAGAAGGTGCTACAGGAATGTGGGTGTCCCTTGAAGGAGGAGGAGCTCATCAGCCTTCTGAA
  GAGCTTGGACGTCAGTGTGCATAACAATCACATCGACCCTGTCGAATTCCTGAGAGCGCTTGAGATCAGC
  TGGGCCAGCAAAGCTCGCCCAAAGGAGAAGGAGGAAAGCTCGCCACCGCCCATCAGCTTCTCTAAGGTGA
  CCCCAGATGAGGTGATAAAGACCATGCAGGAGGTGGTGGAGTCCTCCCAGCCTGCTTTGGTGGAGGCCTT
  CTCTGCACTGGATAAAGAGGACACAGGGTTCGTGAAGGCTATGGAATTTGGAGATGTCCTGCGGAGCGTA
  TGCCAAAAGCTAACGGATAACCAATATCATTACTTCTTGAGGAGATTAAGGCTCCACCTAACACCCAACA
  TACACTGGAAATACTTCCTGGAGAACTTCAGCACCTTCCAGGACGAGACTGCTGACGACTGGGCAGAGAA
  CATGCCCAAGGCCCCGCCCCCCATGTCCCCCAAAGAAACAGCCCACAGAGACATCGTGGCACGAGTGCAG
  AAGGCAGTAGCGTCTCACTACCACACGATTGTCCAGGAGTTTGAGAACTTCGACACCCTGAAAAGCAACA
  CGGTTTCCAGAGACGAGTTCAGGTCCATCTGCACCCGCCACATACAGATCCTGACAGACGAGCAGTTTGA
  CAGACTGTGGAGCGAGCTGCCTGTCAATGCCAAGGGGAGGCTAAAGTACCAGGACTTCCTCAGCAAGTTG
  AGCATCGAGAGGGTGCCCTCGCCGCCCATGGCCGCAGGCGACTCTGGAGAATCGACCATGGCCCAGCGGG
  GAAGCAGCGCCCCTGAGTTCTCCCAAGGGACCAGGTCCAACCTCTACTCGCCACCTCGGGACTCCAGAGT
  GGGGTTGAAATCACGGAGCCACCCCTGTACCCCAGTGGGCACCCCACCCCTACAGAACTGCGAGCCCATC
  GAGAGCCGGCTCCGCAAACAGATCCAGGGCTGCTGGCGGGAGCTGCTGAGGGAGTGCAAGGAGAAAGACA
  CGGACAAGCAGGGGACCATCTCTGCAGCTGAGTTCCTGGCGCTCGTGGAGAAGTTCAAGCTGGACATCAG
  CAGGGAGGAGAGCCAGCAGCTCATCGTCAAGTACGACCTGAAGAACAACGGCAAGTTCGCCTACTGCGAC
  TTCATCCAGAGCTGCGTCCTGCTGCTGAAGGCCAAGGAGACCTCTCTGATGCGGAGGATGAGGATCCAGA
  ATGCGGACAAGATGAAAGAAGCTGGCATGGAGACACCTTCCTTTTACTCGGCCTTGCTACGGATCCAGCC
  TAAGATCGTTCACTGCTGGAGGCCCATGCGGCGTTCCTTCAAGACCTACGACAAGAACGGAACGGGACTC
  CTGAGTGTGGCTGACTTCAGGAAGGTGCTGCGGCAGTACAGCATTAATCTGTCAGAGGAGGAGTTCTTCC
  ACGTGCTCGAGTACTATGACAAATCACTGTCTTCGAAGATTTCCTACAATGACTTCCTGCGTGCCTTCCT
  GCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217033 representing NM_001161629
  Red=Cloning site Green=Tags(s)
  
  MSKKHGCKQKVADSELACEQAHQYLIMKAKTRWADLSKNFIETDNEGNGILRRRDIKNSLYGFDIPLTPR
  EFEKLWQNYDTEGRGYITYQEFLHRLGIRYSPKVHRPYKEDYFNFLGHFTKPKQVQEEIQELQQISEREK
  LMNHYEEISKAFNAMEKSKPVALCRVQKVLQECGCPLKEEELISLLKSLDVSVHNNHIDPVEFLRALEIS
  WASKARPKEKEESSPPPISFSKVTPDEVIKTMQEVVESSQPALVEAFSALDKEDTGFVKAMEFGDVLRSV
  CQKLTDNQYHYFLRRLRLHLTPNIHWKYFLENFSTFQDETADDWAENMPKAPPPMSPKETAHRDIVARVQ
  KAVASHYHTIVQEFENFDTLKSNTVSRDEFRSICTRHIQILTDEQFDRLWSELPVNAKGRLKYQDFLSKL
  SIERVPSPPMAAGDSGESTMAQRGSSAPEFSQGTRSNLYSPPRDSRVGLKSRSHPCTPVGTPPLQNCEPI
  ESRLRKQIQGCWRELLRECKEKDTDKQGTISAAEFLALVEKFKLDISREESQQLIVKYDLKNNGKFAYCD
  FIQSCVLLLKAKETSLMRRMRIQNADKMKEAGMETPSFYSALLRIQPKIVHCWRPMRRSFKTYDKNGTGL
  LSVADFRKVLRQYSINLSEEEFFHVLEYYDKSLSSKISYNDFLRAFLQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001161629
• ORF Size: 2034 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001161629.1, NP_001155101.1
• RefSeq Size: 2412 bp
• RefSeq ORF: 2037 bp
• Locus ID: 77627
• UniProt ID: Q6P1E8
• Cytogenetics: 15 E1-E2
• Gene Summary: Negatively regulates the androgen receptor by recruiting histone deacetylase complex, and protein DJ-1 antagonizes this inhibition by abrogation of this complex.[UniProtKB/Swiss-Prot Function]