Fgd6 (NM_053072) Mouse Tagged ORF Clone

CAT#: MG217013

Fgd6 (tGFP-tagged) - Mouse FYVE RhoGEF and PH domain containing 6 (Fgd6), (10ug)

Product Images

Circular map for MG217013

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fgd6
• Synonyms: AA123052; Etohd4; ZFYVE24
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217013 representing NM_053072
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTGCCGCGGAGCTTAAGAAGCCACCATTGGCTCCCAAGCCAAAGTTAGTGGGGACCAATAATA
  AGCCACCTCCCCCTCCTATTGCACCTAAACCGGACATTGGCAGTGCTAGTGTTCCACGGTTAACGAAGAA
  GACCAAACCGGCAATTGCGCCAAAACCAAAAGTCCCGACAAACTCAGTTGTTCAAGACATTAAGCATCCA
  CCCTCAAAGAAACCTACTTTGAACCTGGAGGAAAGGGAGCCAGAATTACCCGAGAGCACTGGCAAGTCTA
  ATTGCAAGGATGTCAGGGATCCACACAGTGATTATATTTTACCAACGTGTTCCTGCAGTTCTGGGTGTAT
  TCATGAGCCTAGGACTCGAGAAACCCTGTGTGTAGAGCAGCTTGTTTTAGAGCCTCTGGGAATGAAGGAA
  AATTTAGAGAATAGTAAAAATGGTGAGTCCTCAAAAAGGGGGAGTAGCTGGGATTCAAGCAGCGAAAAGT
  GCCGGGGCCAGAGTGGAGTTGTTTTAAAGGCAAGCATCTTAGAAGAGAAGCTCAAAGAGGTCCTGACGCA
  GCAGCGATCACCTTGTGGTTCCCCAGGGAGGCACAGAGCTCCAAAAAAACCAGAAATGAATGGTGACCAT
  AGTTGCACCAGGCAGATCAGAATTGAATTTGCAGATGTGTCTTCTTCCCTGACCGGCTTTGAAAAAGTTC
  CCGCTCATCACAACTGCCACCCACAACTTCCTAGGGATGAATCTCAGACTCTCAAGACTTGTCAAGATGG
  CAGTGCAGAGAGCCATGGTCACACTGATTCATGTGAACCGGAGAACAAAAGAGTGGCTTCAGATGGAATT
  AGTCAGAAGACAGAGGACAAAGGTCTCGGTCCTTTAGAAATCCACTTACTACCGTATACCTCAAAGTTTC
  CAACTCCCAAGCCCAGGAAGACACACGCAGCTGCTCGTCTTCGCCGCCAGAAGCATGTAGATACTCCTGG
  TGAAAGCACTGAAGAGCCAGGGAACTCAAACAATGGCTCTTCTTGTCTCCTGGAGGATAGTTTGAAAAAC
  AATAAAGTCAGTGTTCTTCGTCAGAATGCTTTGTATAACCAGGGACCAGCAGACGAGGTGAGGCCAGCAA
  ACCAGAGGGCATTGACAGGGGATTCCAACAGTGGCGGACAGGACTCAGTCGGCTCACAGAAAGCTGTGCA
  GCAGCAAACGCCTTCTTTAGATACAGATTCTAGTTTGACTTCTGACAGCAGTGGTTCTGGCGTGTCACCT
  GCTGTGGACAAAGAGACTACTTACACACAGTGCAGTACTCAGCCTTTGAGCTTGCCTAAGCAAGTCACAT
  CAGCCTGCACTGACCAGCCACCAGCTACCTGTAACCCGGAAGTGTCAGCTCCTCCCATACAAAAGGAATC
  TTCCTCCTCGAGAATCATTCCCAAAAAACCTCAAAGACACAGCTTGCCTGCAGCAGGGGTGCTTAAAAAG
  GCTGCTTCGGAGGAGCTTGTAGAAAAGAGCTCTTCAGGTAAGGAGACAAATGTCGAGAAAGGTCTGCACA
  GAAACCACCTTCACCATCCGGGGCCCCCAAACCATGGCGCGTCAGCATCACCCTTTGATATGCCCAACCC
  GACTTCAGAGAAGCCAGTGTGGAAGTTACCTCATCCTATTCTACCTTTTTCAGGGAGCCCAGAGGCCTTG
  AAACGCGTCACTTTATCCTTAAATAACGAGCCTTCCGTTTCCCTAACCAAGCCAAGAGCAAAATCCCTGT
  CTGCCGTGGATGCGGACCGGTGTAACAAGCCTTGCAAAGACCCTCCGAAGAAAACTTCTTTTAAAAAGTT
  GATCAATGTGAAACTGTCCATTGGCTTCATAAAGAGCGACTTTCAGAAAATTAGGTCGAAAAGCTGCCAA
  CACGGGGATGTCTCTACAGGACATCCGCTCGCTAGAGAGCCAAAAGGACTTGAAAGTGATTGGCAAGGTT
  TGGCCACAGGAGAAGAGAAGAGAAGTAAGCCCACGAAGGCACATTCTGCAGAAAACTGCAGCCTGGAATC
  TCAAAAGGTGAAGTCCTGGGGCCAGAGCAGTGCAGTTAACGGTCAGAGGGCGGAGTCTTTGGATGACCGG
  ATTCTCTCCAGGCACACGTCCTGTACAGGCGACTTCGGGCCAGAGTATGAAAATGTCCGCCATTATGAGG
  AGATACCCGAGTATGAGAATTTGCCCTTTGTCATGGCTGGAAGGAACACTCCGGACTTGGGATGGCAGAA
  CTCCAGCAGTGTGGAGGACACTGATGCAAGCCTGTATGAAGTGGAAGAGCCCTACAATGCTCCAGATGGC
  CAGCTGCAACTTGATCCTAGACATCAGCCTTGCAGTTCTGGAACATCCCAGGAGGGGCAGGATGCTCTCC
  ATCTTGGCCTCAGTGACCTGCCTTCTGATGAGGAAGTCATCAATAGTTCTGATGAAGATGATGTCAGCTC
  CGAGTCCAGTAAAGGCGAGCCAGACCCCCTGGAAGATAAACAGGATGAAGATGCTGGGATGAAAAGCAAA
  GTTCATCATATTGCCAAGGAGATCATGAGCTCCGAGAAAGTGTTTGTGGATGTGTTAAAGCTTTTGCATA
  TTGACTTCCGGGGTGCTGTAGCCCATGCATCCAGGCAACTCGGGAAGCCCGTGATTGAGGACCGGATCCT
  TAACCAGATCCTGTACTACCTGCCTCAGCTGTACGAGCTCAACCGGGATCTCCTGAAGGAACTGGAAGAG
  CGAATGCTCACCTGGACAGAACAACAAAGAATTGCTGATATCTTTGTAAAGAAGGGGCCATACTTAAAAA
  TGTATTCCACATACATCAAAGAATTTGATAAGAACGTAGCTTTGCTGGATGAGCAGTGCAAGAAAAATCC
  AGGATTTGCTGCAGTTGTCAGAGAATTCGAGATGAGCCCTCGATGTGCCAACCTGGCCCTGAAGCACTAC
  CTGCTAAAGCCAGTTCAGAGGATCCCTCAGTACAGGCTGCTACTGACAGATTACTTAAAGAACCTTCTAG
  AAGACTCTGTAGATCACAGAGACACCCAAGATGCCCTGGCTGTTGTCATAGAGGTAGCTAACCATGCCAA
  TGACACCATGAAGCAAGGGGACAACTTTCAGAAGCTCATGCAGATTCAGTACAGCTTAAGTGGACACCAT
  GAAATTGTGCAACCTGGGCGGGTTTTTCTTAAAGAAGGCACCCTGATGAAGCTGTCTCGGAAAGTCATGC
  AGCCCCGGATGTTTTTCCTGTTTAACGATGCTCTTCTCTACACCACACCCATGCAGTCTGGGATGTACAA
  ACTGAACAACATGCTCTCGCTGGCTGGAATGAAGGTCAGAAAACCTACCCAGGAGGCATATCAGAACGAG
  TTAAAGATTGAAAGTGTAGAGCGTTCTTTCATCCTCTCAGCTAGTTCTGCCGCAGAAAGAGACGACTGGC
  TGGAGGCCATATCCAGCTCCATAGAAGAGTATGCCAAGAAAAGAATCACATTCTGCCCCAGCAGGAGTCT
  TGATGAGGACTCAGAAAGGAAAGAAGAAGTTAGCCCCCTCGGGGCAAAGGCTCCCATCTGGATCCCAGAC
  ACCAGAGCCACCATGTGTATGATTTGCACAAGTGAATTCACCCTGACCTGGAGAAGACACCACTGCAGGG
  CCTGTGGGAAGATTGTGTGCCAAGCTTGCTCATCAAACAAGTATGGCTTAGATTACCTGAAAGGGCAACT
  GGCAAGAGTGTGCGAGCACTGCTTCCAAGAGCTACAGAAATTAGATCACCAGCTGTCGCCTAGGGTTGGG
  TCTCCCGGAAATCACAAGTCTCCTTCAAGTGCCTTGTCGTCAGTCTTACATAGCATCCCGTCAGGGAGGA
  AGCAGAAGAAAATCCCCGCTGCTCTCAAAGAAGTGTCAGCAAACACAGAAGACTCCACTATGAGCGGCTA
  CCTCTACAGATCCAAGGGCAGTAAAAAGCCATGGAAACACTTATGGTTTGTCATCAAAAACAAAGTACTC
  TACACATATGCTGCAAGCGAGGACGTGGCAGCTTTGGAAAGTCAGCCTTTATTAGGCTTCACCGTCACTC
  TAGTCAAGGATGAGAACTCAGAGTCTAAAGTGTTTCAGCTGCTGCACAAAGGCATGGTGTTTTACGTATT
  TAAGGCAGACGATGCCCACTCCACGCAGAGGTGGATAGACGCATTTCAGGAAGGCACAGTATTG
  
  ACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217013 representing NM_053072
  Red=Cloning site Green=Tags(s)
  
  MTSAAELKKPPLAPKPKLVGTNNKPPPPPIAPKPDIGSASVPRLTKKTKPAIAPKPKVPTNSVVQDIKHP
  PSKKPTLNLEEREPELPESTGKSNCKDVRDPHSDYILPTCSCSSGCIHEPRTRETLCVEQLVLEPLGMKE
  NLENSKNGESSKRGSSWDSSSEKCRGQSGVVLKASILEEKLKEVLTQQRSPCGSPGRHRAPKKPEMNGDH
  SCTRQIRIEFADVSSSLTGFEKVPAHHNCHPQLPRDESQTLKTCQDGSAESHGHTDSCEPENKRVASDGI
  SQKTEDKGLGPLEIHLLPYTSKFPTPKPRKTHAAARLRRQKHVDTPGESTEEPGNSNNGSSCLLEDSLKN
  NKVSVLRQNALYNQGPADEVRPANQRALTGDSNSGGQDSVGSQKAVQQQTPSLDTDSSLTSDSSGSGVSP
  AVDKETTYTQCSTQPLSLPKQVTSACTDQPPATCNPEVSAPPIQKESSSSRIIPKKPQRHSLPAAGVLKK
  AASEELVEKSSSGKETNVEKGLHRNHLHHPGPPNHGASASPFDMPNPTSEKPVWKLPHPILPFSGSPEAL
  KRVTLSLNNEPSVSLTKPRAKSLSAVDADRCNKPCKDPPKKTSFKKLINVKLSIGFIKSDFQKIRSKSCQ
  HGDVSTGHPLAREPKGLESDWQGLATGEEKRSKPTKAHSAENCSLESQKVKSWGQSSAVNGQRAESLDDR
  ILSRHTSCTGDFGPEYENVRHYEEIPEYENLPFVMAGRNTPDLGWQNSSSVEDTDASLYEVEEPYNAPDG
  QLQLDPRHQPCSSGTSQEGQDALHLGLSDLPSDEEVINSSDEDDVSSESSKGEPDPLEDKQDEDAGMKSK
  VHHIAKEIMSSEKVFVDVLKLLHIDFRGAVAHASRQLGKPVIEDRILNQILYYLPQLYELNRDLLKELEE
  RMLTWTEQQRIADIFVKKGPYLKMYSTYIKEFDKNVALLDEQCKKNPGFAAVVREFEMSPRCANLALKHY
  LLKPVQRIPQYRLLLTDYLKNLLEDSVDHRDTQDALAVVIEVANHANDTMKQGDNFQKLMQIQYSLSGHH
  EIVQPGRVFLKEGTLMKLSRKVMQPRMFFLFNDALLYTTPMQSGMYKLNNMLSLAGMKVRKPTQEAYQNE
  LKIESVERSFILSASSAAERDDWLEAISSSIEEYAKKRITFCPSRSLDEDSERKEEVSPLGAKAPIWIPD
  TRATMCMICTSEFTLTWRRHHCRACGKIVCQACSSNKYGLDYLKGQLARVCEHCFQELQKLDHQLSPRVG
  SPGNHKSPSSALSSVLHSIPSGRKQKKIPAALKEVSANTEDSTMSGYLYRSKGSKKPWKHLWFVIKNKVL
  YTYAASEDVAALESQPLLGFTVTLVKDENSESKVFQLLHKGMVFYVFKADDAHSTQRWIDAFQEGTVL
  
  TRPLE - GFP Tag - V
• Restriction Sites: SgfI-NotI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053072
• ORF Size: 4197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053072.3, NP_444302.4
• RefSeq Size: 8061 bp
• RefSeq ORF: 4200 bp
• Locus ID: 13998
• UniProt ID: Q69ZL1
• Cytogenetics: 10 C2
• Gene Summary: May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. May play a role in regulating the actin cytoskeleton and cell shape (By similarity).[UniProtKB/Swiss-Prot Function]