Rragd (NM_027491) Mouse Tagged ORF Clone
CAT#: MG216840
- TrueORF®
Rragd (tGFP-tagged) - Mouse Ras-related GTP binding D (Rragd), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_027491" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Rragd |
Synonyms | 5730543C08Rik; AI467523; C030003H22Rik; D4Ertd174e |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216840 representing NM_027491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAAGTGCTGGGCAAGCCGCAGCCGCAGGGTGAGGACGGCGGCGAGGACGAGGAGGAGGATGAGC TGGTGGGGCTGGCGGGCTACGAGGATGGGCCCGAGTCCTCGGACGCCGAGCTGGACAGCGGGCCGGAGGA GGGAGTTCTGGACTTCAGCGACCCCTTCAGCACTGAGGTGAAGCCCAGAATCCTGCTCATGGGTCTCAGG AGGAGCGGCAAGTCGTCTATCCAGAAAGTCGTCTTTCACAAAATGTCCCCGAGCGAGACGCTGTTCTTGG AGAGCACCAACAGGATCTGCCGGGAAGACGTCTCCAACAGCTCCTTCGTCAACTTCCAGATTTGGGACTT CCCAGGGCAGATTGACTTTTTTGACCCTACCTTTGACTATGAGATGATCTTCCGGGGAACCGGGGCACTG ATCTTCGTCATCGACTCCCAGGATGACTATATGGAAGCGCTGGCCCGGCTCCATCTCACAGTGACCAGGG CCTACAAGGTGAACACTGATATCAACTTTGAGGTGTTTATTCATAAAGTAGATGGTCTGTCAGATGACCA CAAAATTGAAACCCAGAGAGACATTCACCAGAGGGCAAACGATGACCTCGCTGATGCTGGATTAGAAAAA ATTCACCTGAGCTTTTACCTGACAAGCATATATGATCATTCAATATTTGAAGCCTTTAGCAAAGTGGTTC AGAAACTGATCCCACAGCTCCCGACGCTGGAGAATCTGCTGAACATCTTTATCTCAAATTCTGGAATTGA GAAAGCGTTTCTGTTTGACGTGGTCAGTAAGATTTATATTGCAACCGACAGCACGCCGGTGGACATGCAG ACGTACGAGCTCTGCTGTGACATGATTGACGTGGTGATCGACATCTCTTGTATTTATGGTCTCAAAGAAG ATGGAGCAGGAGCCCCGTATGACAAGGACTCAACAGCCATTATAAAGCTGAATAATACAACAGTTCTTTA TTTAAAAGAGGTCACAAAGTTCTTGGCGCTCGTTTGCTTCGTCAGAGAGGAAAGCTTTGAAAGAAAAGGG CTGATTGACTATAATTTCCACTGCTTCCGGAAGGCCATCCATGAAGTCTTCGAGGTGAGGATGAAGATGG TGAAATCTCGAAAGGCCCAGAGTCGGCTACCCAAGAAGACAGGAGCTACTCCCAATGGGACCCCTAGAGT GCTGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216840 representing NM_027491
Red=Cloning site Green=Tags(s) MSQVLGKPQPQGEDGGEDEEEDELVGLAGYEDGPESSDAELDSGPEEGVLDFSDPFSTEVKPRILLMGLR RSGKSSIQKVVFHKMSPSETLFLESTNRICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGAL IFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEK IHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQ TYELCCDMIDVVIDISCIYGLKEDGAGAPYDKDSTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKG LIDYNFHCFRKAIHEVFEVRMKMVKSRKAQSRLPKKTGATPNGTPRVLL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_027491 |
ORF Size | 1197 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_027491.2, NP_081767.2 |
RefSeq Size | 5047 bp |
RefSeq ORF | 1200 bp |
Locus ID | 52187 |
UniProt ID | Q7TT45 |
Cytogenetics | 4 14.57 cM |
Gene Summary | Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC209892 | Rragd (untagged) - Mouse Ras-related GTP binding D (Rragd), (10ug) |
USD 503.00 |
|
MR216840 | Rragd (Myc-DDK-tagged) - Mouse Ras-related GTP binding D (Rragd) |
USD 457.00 |
|
MR216840L3 | Lenti ORF clone of Rragd (Myc-DDK-tagged) - Mouse Ras-related GTP binding D (Rragd) |
USD 757.00 |
|
MR216840L4 | Lenti ORF clone of Rragd (mGFP-tagged) - Mouse Ras-related GTP binding D (Rragd) |
USD 757.00 |
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