Rragd (NM_027491) Mouse Untagged Clone

CAT#: MC209892

Rragd (untagged) - Mouse Ras-related GTP binding D (Rragd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rragd
• Synonyms: 5730543C08Rik; AI467523; C030003H22Rik; D4Ertd174e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209892 representing NM_027491
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCAAGTGCTGGGCAAGCCGCAGCCGCAGGGTGAGGACGGCGGCGAGGACGAGGAGGAGGATGAGC
  TGGTGGGGCTGGCGGGCTACGAGGATGGGCCCGAGTCCTCGGACGCCGAGCTGGACAGCGGGCCGGAGGA
  GGGAGTTCTGGACTTCAGCGACCCCTTCAGCACTGAGGTGAAGCCCAGAATCCTGCTCATGGGTCTCAGG
  AGGAGCGGCAAGTCGTCTATCCAGAAAGTCGTCTTTCACAAAATGTCCCCGAGCGAGACGCTGTTCTTGG
  AGAGCACCAACAGGATCTGCCGGGAAGACGTCTCCAACAGCTCCTTCGTCAACTTCCAGATTTGGGACTT
  CCCAGGGCAGATTGACTTTTTTGACCCTACCTTTGACTATGAGATGATCTTCCGGGGAACCGGGGCACTG
  ATCTTCGTCATCGACTCCCAGGATGACTATATGGAAGCGCTGGCCCGGCTCCATCTCACAGTGACCAGGG
  CCTACAAGGTGAACACTGATATCAACTTTGAGGTGTTTATTCATAAAGTAGATGGTCTGTCAGATGACCA
  CAAAATTGAAACCCAGAGAGACATTCACCAGAGGGCAAACGATGACCTCGCTGATGCTGGATTAGAAAAA
  ATTCACCTGAGCTTTTACCTGACAAGCATATATGATCATTCAATATTTGAAGCCTTTAGCAAAGTGGTTC
  AGAAACTGATCCCACAGCTCCCGACGCTGGAGAATCTGCTGAACATCTTTATCTCAAATTCTGGAATTGA
  GAAAGCGTTTCTGTTTGACGTGGTCAGTAAGATTTATATTGCAACCGACAGCACGCCGGTGGACATGCAG
  ACGTACGAGCTCTGCTGTGACATGATTGACGTGGTGATCGACATCTCTTGTATTTATGGTCTCAAAGAAG
  ATGGAGCAGGAGCCCCGTATGACAAGGACTCAACAGCCATTATAAAGCTGAATAATACAACAGTTCTTTA
  TTTAAAAGAGGTCACAAAGTTCTTGGCGCTCGTTTGCTTCGTCAGAGAGGAAAGCTTTGAAAGAAAAGGG
  CTGATTGACTATAATTTCCACTGCTTCCGGAAGGCCATCCATGAAGTCTTCGAGGTGAGGATGAAGATGG
  TGAAATCTCGAAAGGCCCAGAGTCGGCTACCCAAGAAGACAGGAGCTACTCCCAATGGGACCCCTAGAGT
  GCTGCTGTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027491
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027491.2, NP_081767.2
• RefSeq Size: 5047 bp
• RefSeq ORF: 1200 bp
• Locus ID: 52187
• UniProt ID: Q7TT45
• Cytogenetics: 4 14.57 cM
• Gene Summary: Guanine nucleotide-binding protein forming heterodimeric Rag complexes required for the amino acid-induced relocalization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This is a crucial step in the activation of the TOR signaling cascade by amino acids (By similarity).[UniProtKB/Swiss-Prot Function]