Haspin (NM_010353) Mouse Tagged ORF Clone

CAT#: MG216542

Gsg2 (tGFP-tagged) - Mouse germ cell-specific gene 2 (Gsg2), (10ug)

Product Images

Circular map for MG216542

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Haspin
• Synonyms: Gsg2; Haspin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216542 representing NM_010353
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGGCTCACCCGCGGTCGGGTACCCGGCTCTTTCGAACGTACGCGGCGAGGGGCGTCCGGGGGT
  CGCAGCGGCAGCCTGGCGGGCTGGCTGAGCAATGGTTCCAGCCGCCGAACCTGAAGCGCGCCTTCAGCAG
  CAGCCTCAGCGACAGTAACGAGTCGCCGGCTGTCGCTTCCGACGACCCGGACGACCCCGACTTCCCCGGC
  AGCCTCGTGGGCCAGCGGCGGAGGCGTCCTCGGGGCAGCGGCTCCCGGAACCAGCGGACCCTGACAAACA
  CTCCAAGAGTCCAAAGGCTGCGACCTCGGCCCCCGCAGAAGTGCAGCACCCCGTGCAGCCGGCTGCGACC
  GCCACCTTTCCCCAACTGCAGCCCGGGCTGCCTCGGGTCGGACCACAGTGTGTGCATCCAGTCCAGGGAC
  AGCAACGAGCTGGGCACCAGTGCCTCCCTCTTCAGCTCTCCGGCCTCGCCCGGAGCCCCCGACCCTTTGT
  ATGCTGACTCTGCAGTCCCCGGGAGCTTCCACTTGCCGGCAGCCTCCCTAAGTGAACCGTCTGTGCCCTG
  CCCCCAGGTGGCAGCAACCGGTGACAGGTACACCGGGAGGGCCCTCCGAGCCGAAGCCAGCTTCAGGTCA
  TCTCTCTTTAGCCTTGTGAACTCAGGGGCCACAGAAGAGAACAAGTTTGGGACAGATGGGGAGAATGTGA
  AGGAATCGTGTTGTGAAAGAAGACAGCAGATGGGAAACAGACTAACGGACCCTGATCTGACAAGCCCGGG
  AAAGAGAAAGGCCGCGTGTAAAAAGGTTGTGTCCCAAGGAGTCGACCAGAGAGACTATGAGGAGTCCAGT
  GCTTGCAAAGACCTTCGTGTACCAGGGGAAATCAGCAGGCCTAAGAGAACTGGGCCACTCCGGAAGAGGA
  AACGGCAAGAAGCAACGGGAACCCCTCCCCGCCACTACCACCAATCTAAGAAGAAAAGGAAAGCGTCAGT
  CTCCTTATGGAACTTGAATACTAGCCAGAGGGATTCTTGGACCAAAACCAGAGCCTCCTTCGGTTTCCAC
  AAGAAGAAAATTATAACCAGTGTGATAGAGGTATGCAGTTCTGTTGCCAGTTCTTCCTCCAGGTCCCTCC
  TGTCTGAATGTTCGACCCCTCCTATCAAGAACAGAGCACACCTTACTGTTTCTTCTCGATGCTCTTCTGT
  GTATTTGCTAAGTCCCTTAAAGACCCTGCATGTCACAGACCAAAGGCCATCTTATGCTGAAAAGGTTTAT
  GGGGAGTGTAATCAGGAAGGGCCCATCCCCTTTAGTGATTGCCTTTCCACGGAAAAACTGGAACGGTGTG
  AGAAGATTGGGGAAGGAGTGTTTGGAGAAGTGTTTCAGATAATTAACGACCAAGCACCTGTAGCCCTAAA
  AATCATTGCTATTGAAGGGTTAGATTTAGTCAATGGGTCCCACCAGAAAACCTTTGAGGAAATCCTGCCG
  GAGATCATTATCTCCAAAGAGCTGAGTCTCTTGTCTAGTGAGGCCTACAACCGCACAGAAGGCTTTATTG
  GGCTGAACTCAGTACACTGTGTTCAAGGGCTTTACCCTCCCTTGCTGCTGAAAGCCTGGGATCACTATAA
  CACAACCAAAAGATCTGCCAATGACCGGCCTGACTTTTTCCAGGAAGACCAGCTCTTTATTATCCTGGAA
  TTTGAGTTTGGTGGGGTTGACTTGGAGCGAATGAAAACCAAGCTGTCCTCTGTGGCGACTGCAAAGAGCA
  TTCTCCACCAGATCACCGCATCCCTGGCTGTGGCAGAAGCATCCCTGCACTTTGAGCACCGGGACTTACA
  CTGGGGAAATGTGCTCTTAAAGAAAACCAACCTCAAAGAACTCCGCTACACCCTCAATGGGAAAACGAGC
  ACCATTCCCACCCATGGGCTACAGGTCAACATCATTGACTACACCCTGTCCCGCTTGGAGCGGGATGGGA
  TTGTGGTTTTCTGTGATATCTCCGCTGAAGAGGACCTATTTACAGGTGAGGGTGACTACCAGTTTGAGAT
  CTACAGGCTCATGAGGAAGGAGAACAAAAACTGCTGGGGTGAGTATCACCCTTACAATAATGTGCTGTGG
  CTGCATTACCTCACAGACAAGATTCTGAATAAGATGAAATTTAAGACTAAATGCCAGAGCGCAGCCATGA
  AGCAAATAAGGAAAAACCTGCAACATTTCCACAGGACTGTACTGAGTTTCAGCTCTGCCACGGACCTGCT
  CTGCCAACACAGTCTATTTAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216542 representing NM_010353
  Red=Cloning site Green=Tags(s)
  
  MAQAHPRSGTRLFRTYAARGVRGSQRQPGGLAEQWFQPPNLKRAFSSSLSDSNESPAVASDDPDDPDFPG
  SLVGQRRRRPRGSGSRNQRTLTNTPRVQRLRPRPPQKCSTPCSRLRPPPFPNCSPGCLGSDHSVCIQSRD
  SNELGTSASLFSSPASPGAPDPLYADSAVPGSFHLPAASLSEPSVPCPQVAATGDRYTGRALRAEASFRS
  SLFSLVNSGATEENKFGTDGENVKESCCERRQQMGNRLTDPDLTSPGKRKAACKKVVSQGVDQRDYEESS
  ACKDLRVPGEISRPKRTGPLRKRKRQEATGTPPRHYHQSKKKRKASVSLWNLNTSQRDSWTKTRASFGFH
  KKKIITSVIEVCSSVASSSSRSLLSECSTPPIKNRAHLTVSSRCSSVYLLSPLKTLHVTDQRPSYAEKVY
  GECNQEGPIPFSDCLSTEKLERCEKIGEGVFGEVFQIINDQAPVALKIIAIEGLDLVNGSHQKTFEEILP
  EIIISKELSLLSSEAYNRTEGFIGLNSVHCVQGLYPPLLLKAWDHYNTTKRSANDRPDFFQEDQLFIILE
  FEFGGVDLERMKTKLSSVATAKSILHQITASLAVAEASLHFEHRDLHWGNVLLKKTNLKELRYTLNGKTS
  TIPTHGLQVNIIDYTLSRLERDGIVVFCDISAEEDLFTGEGDYQFEIYRLMRKENKNCWGEYHPYNNVLW
  LHYLTDKILNKMKFKTKCQSAAMKQIRKNLQHFHRTVLSFSSATDLLCQHSLFR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010353
• ORF Size: 2262 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010353.2, NP_034483.1
• RefSeq Size: 2818 bp
• RefSeq ORF: 2265 bp
• Locus ID: 14841
• UniProt ID: Q9Z0R0
• Cytogenetics: 11 45.25 cM
• Gene Summary: Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. May act through H3T3ph to both position and modulate activation of AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.[UniProtKB/Swiss-Prot Function]