Krt9 (NM_201255) Mouse Tagged ORF Clone

CAT#: MG216510

Krt9 (tGFP-tagged) - Mouse keratin 9 (Krt9), (10ug). Note: ORF is codon optimized

Product Images

Circular map for MG216510

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Krt9
• Synonyms: EPPK; K9; Krt1-9
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216510 representing NM_201255, codon optimized.
  Due to the complexity of NM_201255, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTGCCGCCAGAGTAGTTCATCTTTTTGGTCTTCCTCTAGTAGCTGTGGGGGCGGAGGCGGTAGAG
  GTGGATCTGGAGGGTCCATGAGGAGCAGCTTCAGCAGAAGCAGTAGAGCGGGAGGGGGCGGCGGAGGCAG
  GTTTAATAGTTCAAGTGGCTTTAGTGGGGGTGGCTTCTCAGCCTGCGGGGGTGGGGGAGGTGGGTCCTTT
  GGGAGCTCCTACGGAGGGGGGTATGGCGGCGGTTTCTCTACCGGCAGTTACAGCGGCATGTTCGGGGGAG
  GGTCCGGCGGGGGCTTCGGGGGAGGGAGTGGAGGCGGGTTCGGTGGAGGCAGCGGAGGTGGCTTTGGAGG
  TGGAAGCGGAGGCGGCGAAGGCGGCATCCTCAACACCAATGAGAAGATCGTTATGCAAAACCTGAACAGC
  AGACTGGCATCTTATATGGAGAAGGTGCTGGAGCTGGAAGAATCAAACACTGCCATGGAGAAACAGATAC
  AAGACTGGTATAGCAAGCGAGGCCCTAAGGTCTTCCAGAAGGACAATACTCACTATTACGACACTATTGA
  GGACCTTAAGGACAGAATCGTTGACCTCACAGTACGCAACAATAAAACCCTGGTGGACATTGACAACACC
  AGAATGACTATGGATGACTTCAGAGTGAAACTCGAAATGGAGCAGTCCCTGAGACAAGGGGTCGAGGGGG
  ACATCAACGGTCTGAAGAAGGTGCTTGATGATCTTGTCATGGCCAAGAGTGATCTGGAGATACTGCTGGA
  TAGCCTGGAGGACGAAAAGAACGCACTGACTAAAAACCATAAGGAGGAGATGTCCCAGCTTACAGGCCAG
  AATGATGGTGACGTTAATGTAGAAATAAACGTGGCCCCATCAACCGACCTCACCAGGGTGCTCAATGACA
  TGCGCGAAGAATACGAGCAGCTCATCTCCAAAAATCGCCAGGACATTGAACAGCATTACGAAAGCAAAAT
  GACACAGATCGAGCAGCAGATGACCAACAGCGGGCAGGAAATGGAATCTAATATGAAGCAGGTTAGCCAG
  CTGCAGCACACAATCCAGGAACTGAATGTGGAGTTGCAGACCCAGCTGACCACCAAATCTGCCCTGGAAA
  AGGCTCTGGAAGACACGAAAAACCGATACTGTGGCCAATTGCAACAAATACAGGAGCAGATTAGCGAATT
  GGAGGCTCAGCTGGCTGAAATTAGAGCTGAAACAGAGTGCCAGAGTCAGGAATACTCCATCCTGCTCAGC
  ATCAAGACCAGGCTGGAAAAGGAGATTGAGACATATCGGGAGTTGCTGGAAGGCGGACAGCAGGATTTTG
  AATCCAGCGGTGCCGGACAGATTGGATTTGGAAGCGGAAAGGGACGACAACGCGGGAGTGGCGGGTCCTA
  CGGAGGGGGAAGCGGCGGATCTTACGGCGGTGGGAGCGGCGGCAGCTATGGTGGTGGGAGTGGCGGATCC
  TACGGAGGAGGATCTGGGGGTAGCTACGGTGGAGGTAGTGGGGGATCTCACGGCGGTAAAAGTGGTGGAA
  GTCACGGAGGAGGTTCCGGCGGTAGTTATGGAGGAGAGAGTGGTGGCTCTCACGGCGGAGGGTCCGGAGG
  ATCTTACGGAGGGGGTTCCGGTGGCAGCCACGGAGGCAAGAGCGGCGGTGGATACGGAGGTGGAAGCAGC
  TCCGGGGGGGGGAGCGGGGGCAGCTACGGCGGCGGATCAGGAGGCTCCCACGGTGGCGGGAGTGGCGGTT
  CATATGGCGGTGGGTCAGGAGGAAGCCATGGAGGAAAATCTGGTGGCGGCTACGGAGGAGGCAGCTCCAG
  CGGGGGAGGTTCAGGCGGCTCCTATGGAGGAGGGTCTGGAGGGTCACACGGCGGGAAGTCCGGAGGAAGC
  TACGGGGGTGGATCTGGTGGTAGCTACGGAGGCGGCTCTGGTGGTAGTCACGGCGGAAAGAGCGGCGGCG
  GATATGGCGGCGGCAGCTCCTCCGGAGGCGGGTCTGGAGGCTCATACGGGGGAGGGAGCGGCGGGTCACA
  CGGCGGGAAGTCTGGGGGAAGCTACGGCGGGGGTTCCAGTTCCGGAGGCGGGTCAGGCGGTAGTTACGGG
  GGAGGCAGTGGCTCTGGCGGAGGATCAGGCGGATCTTACGGTGGAGGGAACCGACGCCCAAGCCAATCCC
  AGTCAAGCAGCAAATCCGCAGACTGTGACGACGACTCTCAGGAGCACAAGATGCGGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216510 representing NM_201255
  Red=Cloning site Green=Tags(s)
  
  MSCRQSSSSFWSSSSSCGGGGGRGGSGGSMRSSFSRSSRAGGGGGGRFNSSSGFSGGGFSACGGGGGGSF
  GSSYGGGYGGGFSTGSYSGMFGGGSGGGFGGGSGGGFGGGSGGGFGGGSGGGEGGILNTNEKIVMQNLNS
  RLASYMEKVLELEESNTAMEKQIQDWYSKRGPKVFQKDNTHYYDTIEDLKDRIVDLTVRNNKTLVDIDNT
  RMTMDDFRVKLEMEQSLRQGVEGDINGLKKVLDDLVMAKSDLEILLDSLEDEKNALTKNHKEEMSQLTGQ
  NDGDVNVEINVAPSTDLTRVLNDMREEYEQLISKNRQDIEQHYESKMTQIEQQMTNSGQEMESNMKQVSQ
  LQHTIQELNVELQTQLTTKSALEKALEDTKNRYCGQLQQIQEQISELEAQLAEIRAETECQSQEYSILLS
  IKTRLEKEIETYRELLEGGQQDFESSGAGQIGFGSGKGRQRGSGGSYGGGSGGSYGGGSGGSYGGGSGGS
  YGGGSGGSYGGGSGGSHGGKSGGSHGGGSGGSYGGESGGSHGGGSGGSYGGGSGGSHGGKSGGGYGGGSS
  SGGGSGGSYGGGSGGSHGGGSGGSYGGGSGGSHGGKSGGGYGGGSSSGGGSGGSYGGGSGGSHGGKSGGS
  YGGGSGGSYGGGSGGSHGGKSGGGYGGGSSSGGGSGGSYGGGSGGSHGGKSGGSYGGGSSSGGGSGGSYG
  GGSGSGGGSGGSYGGGNRRPSQSQSSSKSADCDDDSQEHKMRY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201255
• ORF Size: 2229 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201255.2, NP_957707.2
• RefSeq Size: 2580 bp
• RefSeq ORF: 2232 bp
• Locus ID: 107656
• UniProt ID: Q6RHW0
• Cytogenetics: 11 D
• Gene Summary: May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly (By similarity). Plays an essential role in the correct development of sperm.[UniProtKB/Swiss-Prot Function]