Samd9l (NM_010156) Mouse Tagged ORF Clone

CAT#: MG216450

Samd9l (tGFP-tagged) - Mouse sterile alpha motif domain containing 9-like (Samd9l), (10ug)

Product Images

Circular map for MG216450

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Samd9l
• Synonyms: AA175286; ESTM25; mKIAA2005
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216450 representing NM_010156
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGGACAAGTAACTCAACCAAAATTGATCAAAGACTGGACCAAAGAACATGTAAGAAAATGGGTTA
  CTGAAGATCTTAACATTGTTGAGAAATATGCTCAAATCTTGTTCAAAGAAGAGGTAACAGGGATGGTCCT
  GCAGGAATTAACTGAAGAGGATCTTAGAGAAATGGGCTTGCCACGGGGTCCAGCACTTCTGATAAAACGC
  ATGTATAACAAACTAATTAGTTCCCCTGAAAGTCACAATCAAGATTCCAGAGAATTAAATGATAAAAAAC
  TCTCAACAAAAGAACAGCAAACAAAGACCAAAAATGAGGAAGAAAACTCAGTTTCATCCAACAGTGATCA
  TGGTCTCAGGGAGACAGGGCAGAATGAAGAACAAGAACCAAGTCTTACGAAAGAAAACATGTTAGGTGAT
  GTGGTGACTAAAGACATGGAAGATAATAAGCCCAAACCAGAACAGATGTCTTGCACGCCATACCCTTTTG
  ATTCTTTTTGTGATGTCAAACAATACATAGAACATTCTATTCTACGTGTTGCTGAGACAGGGCCACTCAA
  TCTCATTGACCCAATACATGAATTCAAAGCCTTTACAAACACAAAGAAAGCCACAGAAGAGGACATAAAA
  ATGAAATTTAGCAATGAGACTTTCCGATTTGCAGCAGCTTGTATGAATTCACGTACCAATGGCACTATTC
  ATTTTGGAGTAAAAGACAAACCACACGGGGAAATTGTTGGTGTGCAAGTCACCAGTAAAGATATCTTTGT
  TAACCATTTCAACACAATGATCACGAAGTATTTTGAAGACAGTGAAATCAGTGAAGCCAGGGCTTGTATC
  CGGGAACCACGGTTCGTGGAAGTCCTTTTGCAGAATAATACACAATCTAATAGATTTGTCATTGAGGTAG
  ATGTTATTCCCAGACATTCTATATGTCAAGAAAAGTATTTCTACATTATGATGCAAAGTAGCACAGGCAA
  AACATGGAAACAAAGCAAAGATACTTCATTGTTTGTAAGAGAAGGAGCTAGCTCTAAGAATATCCTGGGC
  AATCCCAACCAACGGGATAGAGAGTTCAAGAAATTTCTAGAGGATTTAAAGATGTGGACAGCGTCTAGAA
  AAGCGGCCGAAGAAGAACTTAGAATGGTAACTAAGAAAGAGAGTGAAGGACTAAAACTGTCTAAACTTTT
  GACCAGACACCAAGGCTCACTTGATGAATCTTACTATGATTGGTACATTCTTGTAACAAATACCTGTGCA
  CCAACTCAATTAGAGCACTTAGAGTTCATAAAGGAAATGAAATTGTTTGCTGTGCTAGATTTTGATCCTT
  ATTCTCACATCAAGGGAGTGGTCAAAGCTTACAGAGAAAGCAGAATAGCAAACCTTCACCTGCCCAGTCA
  CTATGAGGAAAAAACTACCATAGCAGAGAAAATTTCTACTCTGAAACTTTATGAGCAACCTAGCTGGATC
  TTCTGCAATGGCAGAGTAGACTTGTCATGTCAACCTCTAGAGCCACATTTATGGCAGAGAGATAGAGCTT
  CTGGGGTTAGAAGACTTATTTCATTCCTCACAGACGAAAATATCATAGTAAAAGGGAAGGTTTTGGTGGT
  GTTCCTCTTACTTTCTCCCATAGAAAACCAAAAAGATCCACTCATTGAGACTTTTTGTGCTTTCTATCAA
  GTTTTCAATGGAATGGACAATATGTTGTGTATCTGTGTCAACTCAGCTATCTACCAACAATGGAGTGATC
  TACTACAAGTAAGACTGGAAATCAAAGATGATCTGGCAAAACATAGCATTTCCACTTTAAACATAGAACT
  AGTCAACAATACCATCCTCAAACTGAAATCAGTGATTCAATCTTCAAGGAGATTTTTGCCCTCCTGTGGC
  TCCTCCTCTGTTATTCTGGAGAAAATGGATGAAGATATCATGAGTGCATTGGAAATCCTCTGTGAAAATG
  AGTGTAAAGACACAGACATAGAGAAAGATGAATCTCAATTCCTAGAATTTAAGAAATCAAGAGAAGAACA
  CTTTTATCGAGGAGGCAGAGTATCCTGGTGGAATTTCTATTTTTCTTCTGAAAACTATTCTTCAGCTTTT
  GTAAAAAGGGATAGTTTTGAAGAGCTAACAACATTAATACAACAGTGTGCAGACTCTCCTAAACCAGTGT
  TTGTCAAAGTCATCAACCTGTACCATCACCCAGGCTGTGGAGGTACTACGTTGGCGATGCATGTTCTCTG
  GGACTTAAAGCAGAAGTTTCGGTGTGCTGTCTTGAAAAACAAAGCAACTGATTTTGTAGAGATTGGAGAA
  CAAGTAAGCAAGCTAATGAGCTACAAGGCTACCAGCCACGAGGACTTCATTCCCGTTCTTCTCCTTGTGG
  ATGATTTCGAAGAACAAGAGAACGCCTATATTCTGCAGAATGCCATCAATGCCTTTATAGCAGAAAAGGG
  TCTGAGATATGAAAAAACATTAGTCATAATCCTGAACTGCATGAGATCTCAGAATCCAGATGAAAGTGCA
  AAGTTAGCAAATAGCATTTCACTAAAATACCAACTTTCTCCAAAAGAACAAAGAGCCTTTGAGGCCAAAC
  TACAGGAAATTGAAAAGGAGCATAAGAACTGTGAAAACTTTTATTCGTTCATGATCTTGAAAGGCAACTT
  TGATACAACTTACATAAAGAATGTAGTGAAGAATACTCTGAAGGACCTGGATGCTAAAAGCAGGAGGGCC
  CAGCTCATTTCTTACCTGGCATTACTCAACTCTTATGTTACAGATTCTACCATTTCAGTATCACAGTGTG
  AAATATTTTTGGGAATCACTTACACCAAGAAATATGGGAAACCTGAAACTGTAGAAAAGAACATGGGAAC
  CTATTCCACACTTCTAATAAGAACTGAAGTTTCAGATTATGGGAGGTACACTGGTATACGTATCATTCAC
  CCTCTAATTGCCACCCACTGTTTAAAAGAACTGGAAATGAGCTACAGAATGGATAAGTGTCAGATTGCAC
  TAAATATGCTAGAAGAGAATGTATTGTATGATTCTGGTCTAGGAAGGGACAAATTTAAATATGATGTGCA
  AACTCTATTGCTTACAAGACAACGCAAGGAACATGGAGCTGAAACAGACACTCTCTTTTCCCCATTGATT
  GAAGAATTGCAGAATGAGGAAACTGAAAAGGTCTTGATCGCAGGAAGTGATCGATTCCCACAAAATGCAT
  TCATTTGTCAAGCTTTAGCAAGACACTTCTATATTAAAGAGAAGAACTTTAGCACTGCTTTAGTCTGGGC
  AAATCTGGCCAAAAGGAAAGCACCTAAGAATTCTTATATTTCAGATACACTTGGTCAAGTTTACAAAAGT
  GAGCTCAAACAGTGGTTGGGTAAAAATAAGACATGTGGAAATATTAGTGTGGATGATCTGGCCTATTTCC
  TAGAAGTTGCTGAAAAGGCTTCAAAAGCCTTCAAAGAGTCTCAGAATCAATCTGATAGTAAGGACTATGG
  AACTGAGGCATGGTCACCACAGAATTCCCAAAGAAGATATGACACTTTCAACACAGCCGGCTTCTTCGGA
  GAAATAGAAGTAGGTCTTGACACTATCCAGCTTCTTCAGCTCACACCCCTTTTCCATAAAGAAAATGAAA
  TATCAAAAGAATCTATGGCAGAGTTTTTATCTGGAAAGGGGACCATCCTTTCAGACCCAAAAGGTGAATA
  TTGTGTTGTTCTCAGCAAATTTACATCCCTTCTACAGAATTTACATTCAGATTTGGAAAGATGTTTTCAT
  TTTTTTGGTGATTATATGGGTTTTCTGAAACCAAGGAATACTCCAAAAGAATTGACAGAACTCTTACTAA
  GTAAGAAAGTCAGCCGCTGTTTCAAGAAATATGTAGAACTTTTCTGCCATTTAGATACCAACTTGGTACA
  GGGCAAAGAAGATCTGTTACTTCAAAAGGAGAACTGTAGGAAGCGCATACAAGCTTGGCGAGCAGATACA
  TTTTCAGGACTCCTTGAATATCTTAATCCAAATCACAAAGAAGCTAACAACATAGAAAATATAGTGGGAA
  ACTATACCTTCCTATTACAGGATATCCTAAACAAGCAACTGTCAAAGGTGCTGACCAAGGACATACAAAA
  TTTCATTTTGGCCAACATTATTCTCAGTTGTCTAAAGCCCAGTTCCAAGTATATCTTGCCATTTAGCACA
  CTGAAAAAAAAGCTCAGAGAGGTCCTGCAGATTGTGGGGCTAACTCATTCATATCCTGATCCTTATTTCT
  TGGCCTGCCTCCTATTCTGGCCAGAAAACAAAGAGCTAGATGAAGACTCCACACTAATAGAAAAGTATGT
  CTCATCCTTGAATCGATCCTTCAGGAGACAGTACAAGCACATGTGTAGGTCCAGGCAGCCAAGCACACTC
  TTCTACCTGGGGCAGAAGAAGGGTCTTAACAGTCTTGTTCATAAGGCTGAAATCGAGCGCTACTTCAGTG
  AAGTGCAAGATTCAAATTCCTTCTGGCACAGTGGAGTAGTGTGGGAAAAAAGAGAGGTCAAAGACCTCCT
  ACGCCTGCTGGATGGCCAGGCTGAAGGCAAACTAATCTCACTAGAATATGGAACAGAGGCAAAAATAAAA
  ATACCAGTGACTTCTGTGTATTCAGCTCCACTCAGAAGTGGAAGGAACATAGAGAGAGTGTCCTTCTACC
  TAGGATTTTCCATTGAGGGTCCTCTGGCATATGGCATAAAAGTGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216450 representing NM_010156
  Red=Cloning site Green=Tags(s)
  
  MSGQVTQPKLIKDWTKEHVRKWVTEDLNIVEKYAQILFKEEVTGMVLQELTEEDLREMGLPRGPALLIKR
  MYNKLISSPESHNQDSRELNDKKLSTKEQQTKTKNEEENSVSSNSDHGLRETGQNEEQEPSLTKENMLGD
  VVTKDMEDNKPKPEQMSCTPYPFDSFCDVKQYIEHSILRVAETGPLNLIDPIHEFKAFTNTKKATEEDIK
  MKFSNETFRFAAACMNSRTNGTIHFGVKDKPHGEIVGVQVTSKDIFVNHFNTMITKYFEDSEISEARACI
  REPRFVEVLLQNNTQSNRFVIEVDVIPRHSICQEKYFYIMMQSSTGKTWKQSKDTSLFVREGASSKNILG
  NPNQRDREFKKFLEDLKMWTASRKAAEEELRMVTKKESEGLKLSKLLTRHQGSLDESYYDWYILVTNTCA
  PTQLEHLEFIKEMKLFAVLDFDPYSHIKGVVKAYRESRIANLHLPSHYEEKTTIAEKISTLKLYEQPSWI
  FCNGRVDLSCQPLEPHLWQRDRASGVRRLISFLTDENIIVKGKVLVVFLLLSPIENQKDPLIETFCAFYQ
  VFNGMDNMLCICVNSAIYQQWSDLLQVRLEIKDDLAKHSISTLNIELVNNTILKLKSVIQSSRRFLPSCG
  SSSVILEKMDEDIMSALEILCENECKDTDIEKDESQFLEFKKSREEHFYRGGRVSWWNFYFSSENYSSAF
  VKRDSFEELTTLIQQCADSPKPVFVKVINLYHHPGCGGTTLAMHVLWDLKQKFRCAVLKNKATDFVEIGE
  QVSKLMSYKATSHEDFIPVLLLVDDFEEQENAYILQNAINAFIAEKGLRYEKTLVIILNCMRSQNPDESA
  KLANSISLKYQLSPKEQRAFEAKLQEIEKEHKNCENFYSFMILKGNFDTTYIKNVVKNTLKDLDAKSRRA
  QLISYLALLNSYVTDSTISVSQCEIFLGITYTKKYGKPETVEKNMGTYSTLLIRTEVSDYGRYTGIRIIH
  PLIATHCLKELEMSYRMDKCQIALNMLEENVLYDSGLGRDKFKYDVQTLLLTRQRKEHGAETDTLFSPLI
  EELQNEETEKVLIAGSDRFPQNAFICQALARHFYIKEKNFSTALVWANLAKRKAPKNSYISDTLGQVYKS
  ELKQWLGKNKTCGNISVDDLAYFLEVAEKASKAFKESQNQSDSKDYGTEAWSPQNSQRRYDTFNTAGFFG
  EIEVGLDTIQLLQLTPLFHKENEISKESMAEFLSGKGTILSDPKGEYCVVLSKFTSLLQNLHSDLERCFH
  FFGDYMGFLKPRNTPKELTELLLSKKVSRCFKKYVELFCHLDTNLVQGKEDLLLQKENCRKRIQAWRADT
  FSGLLEYLNPNHKEANNIENIVGNYTFLLQDILNKQLSKVLTKDIQNFILANIILSCLKPSSKYILPFST
  LKKKLREVLQIVGLTHSYPDPYFLACLLFWPENKELDEDSTLIEKYVSSLNRSFRRQYKHMCRSRQPSTL
  FYLGQKKGLNSLVHKAEIERYFSEVQDSNSFWHSGVVWEKREVKDLLRLLDGQAEGKLISLEYGTEAKIK
  IPVTSVYSAPLRSGRNIERVSFYLGFSIEGPLAYGIKVI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010156
• ORF Size: 4737 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010156.3, NP_034286.2
• RefSeq Size: 5344 bp
• RefSeq ORF: 4740 bp
• Locus ID: 209086
• Cytogenetics: 6 1.76 cM