Slc27a3 (NM_011988) Mouse Tagged ORF Clone

CAT#: MG216437

Slc27a3 (tGFP-tagged) - Mouse solute carrier family 27 (fatty acid transporter) member 3 (Slc27a3), (10ug)

Product Images

Circular map for MG216437

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc27a3
• Synonyms: Acsvl3; FATP-3; FATP3; Vlcs-3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216437 representing NM_011988
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCCTCCTGCTGCTGCTGCCCTTACTCTTGCTCCTGCCTCTGCTGCTGAAGCTAGACGTCTGGC
  CTCAGCTGCGCTGGCTGCCGGCCGACCTGGCCTTCACAGTTCGCGCCCTCCGCTGCAAAAGGGCCCTTCG
  AGCACGCGCGCTGGCCGCAGCGGCCGCCGACCCCGAAAGCTCTGAGAGCGGGTGCAGTCTGGCCTGGCGT
  CTCGCGTACCTGGCCCGGGAGCAGCCGACACACACCTTCCTCATCCACGGCGCGCAGCGCTTTAGCTACG
  CGGAGGCTGAGCGCGAGAGCAACCGGATTGCTCGCGCCTTTCTGCGCGCACGGGGCTGGACCGGGGGCCG
  CCGAGGCTCGGGCAGGGGCAGCACTGAGGAAGGCGCACGCGTGGCGCCTCCGGCTGGAGATGCGGCTGCT
  GGAGGGACGACCGCGCCCCCTCTGGCACCCGGGGCGACCGTGGCGCTGCTCCTCCCAGCGGGCCCGGATT
  TCCTTTGGATTTGGTTCGGACTGGCCAAAGCTGGCCTGCGCACGGCCTTTGTGCCCACCGCTTTACGCCG
  AGGACCCCTGCTGCACTGCCTCCGCAGCTGCGGTGCGAGTGCGCTCGTGCTGGCCACAGAGTTCCTGGAG
  TCCCTGGAGCCGGACCTGCCGGCCTTGAGAGCCATGGGGCTCCACCTATGGGCGACGGGCCCTGAAACTA
  ATGTAGCTGGAATCAGCAATTTGCTATCGGAAGCAGCAGACCAAGTGGATGAGCCAGTGCCGGGGTACCT
  CTCTGCCCCCCAGAACATAATGGACACCTGCCTGTACATCTTCACCTCTGGCACTACTGGCCTGCCCAAG
  GCTGCTCGAATCAGTCATCTGAAGGTTCTACAGTGCCAGGGATTCTACCATCTGTGTGGAGTCCACCAGG
  AGGACGTGATCTACCTCGCACTCCCACTGTACCACATGTCTGGCTCCCTTCTGGGCATTGTGGGCTGCTT
  GGGCATTGGGGCCACCGTGGTGCTGAAACCCAAGTTCTCAGCTAGCCAGTTCTGGGACGATTGCCAGAAA
  CACAGGGTGACAGTGTTCCAGTACATTGGGGAGTTGTGCCGATACCTCGTCAACCAGCCCCCGAGCAAGG
  CAGAGTGTGACCATAAGGTGCGCTTGGCAGTGGGCAGTGGGTTGCGCCCAGACACCTGGGAGCGTTTCCT
  GCGGCGATTTGGACCTCTGCAGATACTGGAGACGTATGGCATGACAGAGGGCAACGTAGCTACGTTCAAT
  TACACAGGACGGCAGGGTGCAGTGGGGCGAGCTTCCTGGCTTTACAAGCACATCTTCCCCTTCTCCTTGA
  TTCGATACGATGTCATGACAGGGGAGCCTATTCGGAATGCCCAGGGGCACTGCATGACCACATCTCCAGG
  TGAGCCAGGCCTACTGGTGGCCCCAGTGAGCCAGCAGTCCCCCTTCCTGGGCTATGCTGGGGCTCCGGAG
  CTGGCCAAGGACAAGCTGCTGAAGGATGTCTTCTGGTCTGGGGACGTTTTCTTCAATACTGGGGACCTCT
  TGGTCTGTGATGAGCAAGGCTTTCTTCACTTCCACGATCGTACTGGAGACACCTTCAGGTGGAAGGGAGA
  GAATGTGGCCACAACTGAAGTGGCTGAGGTCTTGGAGACCCTGGACTTCCTTCAGGAGGTGAACATCTAT
  GGAGTCACGGTGCCAGGGCACGAAGGCAGGGCAGGCATGGCGGCCTTGGCTCTGCGGCCCCCGCAGGCTC
  TGAACCTGGTGCAGCTCTACAGCCATGTTTCTGAGAACTTGCCACCGTATGCCCGACCTCGGTTTCTCAG
  GCTCCAGGAATCTTTGGCCACTACTGAGACCTTCAAACAGCAGAAGGTTAGGATGGCCAATGAGGGCTTT
  GACCCCAGTGTACTGTCTGACCCACTCTATGTTCTGGACCAAGATATAGGGGCCTACCTGCCCCTCACAC
  CTGCCCGGTACAGTGCCCTCCTGTCTGGAGACCTTCGAATC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216437 representing NM_011988
  Red=Cloning site Green=Tags(s)
  
  MAALLLLLPLLLLLPLLLKLDVWPQLRWLPADLAFTVRALRCKRALRARALAAAAADPESSESGCSLAWR
  LAYLAREQPTHTFLIHGAQRFSYAEAERESNRIARAFLRARGWTGGRRGSGRGSTEEGARVAPPAGDAAA
  GGTTAPPLAPGATVALLLPAGPDFLWIWFGLAKAGLRTAFVPTALRRGPLLHCLRSCGASALVLATEFLE
  SLEPDLPALRAMGLHLWATGPETNVAGISNLLSEAADQVDEPVPGYLSAPQNIMDTCLYIFTSGTTGLPK
  AARISHLKVLQCQGFYHLCGVHQEDVIYLALPLYHMSGSLLGIVGCLGIGATVVLKPKFSASQFWDDCQK
  HRVTVFQYIGELCRYLVNQPPSKAECDHKVRLAVGSGLRPDTWERFLRRFGPLQILETYGMTEGNVATFN
  YTGRQGAVGRASWLYKHIFPFSLIRYDVMTGEPIRNAQGHCMTTSPGEPGLLVAPVSQQSPFLGYAGAPE
  LAKDKLLKDVFWSGDVFFNTGDLLVCDEQGFLHFHDRTGDTFRWKGENVATTEVAEVLETLDFLQEVNIY
  GVTVPGHEGRAGMAALALRPPQALNLVQLYSHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGF
  DPSVLSDPLYVLDQDIGAYLPLTPARYSALLSGDLRI
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011988
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011988.2, NP_036118.2
• RefSeq Size: 2284 bp
• RefSeq ORF: 2004 bp
• Locus ID: 26568
• UniProt ID: O88561
• Cytogenetics: 3 F1
• Gene Summary: Has acyl-CoA ligase activity for long-chain and very-long-chain fatty acids. Does not exhibit fatty acid transport activity.[UniProtKB/Swiss-Prot Function]