Cyth3 (NM_001163548) Mouse Tagged ORF Clone

CAT#: MG216043

Cyth3 (tGFP-tagged) - Mouse cytohesin 3 (Cyth3) transcript variant 2, (10ug)

Product Images

Circular map for MG216043

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyth3
• Synonyms: AI648983; ARNO3; CLM3; Grp1; Pscd3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216043 representing NM_001163548
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAAGGCGGTGGCGGTGAGGGCGGCAGCGTGCCTGAAGACCTGTCATTAGAAGAGCGAGAAGAAC
  TTTTGGACATTCGTAGAAGAAAAAAGGAACTTATTGATGACATTGAGAGGCTGAAATATGAAATTGCAGA
  AGTGATGACGGAGATTGACAACCTGACTTCAGTGGAGGAGAGCAAAACTACTCAGAGGAACAAGCAAATA
  GCCATGGGAAGGAAGAAATTCAACATGGACCCCAAAAAGGGCATTCAGTTCCTAATTGAGAACGACCTGC
  TGCAGAGCTCCCCAGAGGATGTCGCCCAGTTTCTGTACAAAGGAGAGGGCCTGAACAAGACCGTCATCGG
  AGACTACCTGGGTGAGAGGGATGACTTTAATATCAAAGTTCTTCAGGCTTTTGTTGAGCTGCATGAGTTT
  GCTGATCTCAACCTTGTCCAGGCCTTAAGGCAGTTCCTATGGAGCTTCAGACTTCCTGGAGAGGCACAGA
  AGATCGACCGCATGATGGAGGCCTTTGCATCCCGATACTGCCTGTGCAACCCTGGGGTCTTCCAGTCCAC
  AGATACATGCTACGTGCTCTCCTTTGCCATCATCATGCTCAACACCAGCTTGCACAACCACAACGTGCGC
  GACAAGCCCACCGCTGAGCGCTTCATCACCATGAACCGAGGCATCAACGAGGGTGGGGACCTTCCTGAGG
  AGCTGCTGAGGAACTTGTATGAAAGTATCAAGAATGAGCCGTTTAAGATCCCAGAAGACGACGGAAATGA
  CCTGACACACACGTTCTTCAACCCAGACCGAGAAGGCTGGCTGCTGAAGCTGGGGGGTCGTGTGAAGACC
  TGGAAACGGCGCTGGTTCATCCTCACAGATAACTGCCTCTACTACTTTGAGTACACCACGGACAAGGAGC
  CCAGGGGCATCATCCCCCTGGAGAACCTCAGCATCAGGGAGGTGGAGGACCCCCGGAAGCCGAACTGCTT
  TGAGCTGTATAACCCCAGTCACAAAGGTCAAGTCATCAAGGCCTGCAAGACGGAGGCCGATGGCCGTGTG
  GTGGAGGGCAACCACGTTGTGTACCGGATCTCTGCCCCCAGCCCGGAGGAAAAGGAGGAGTGGATGAAGT
  CCATCAAAGCAAGCATCAGTAGGGACCCGTTCTATGACATGTTGGCCACGAGGAAAAGGAGGATTGCCAA
  TAAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216043 representing NM_001163548
  Red=Cloning site Green=Tags(s)
  
  MDEGGGGEGGSVPEDLSLEEREELLDIRRRKKELIDDIERLKYEIAEVMTEIDNLTSVEESKTTQRNKQI
  AMGRKKFNMDPKKGIQFLIENDLLQSSPEDVAQFLYKGEGLNKTVIGDYLGERDDFNIKVLQAFVELHEF
  ADLNLVQALRQFLWSFRLPGEAQKIDRMMEAFASRYCLCNPGVFQSTDTCYVLSFAIIMLNTSLHNHNVR
  DKPTAERFITMNRGINEGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKT
  WKRRWFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDPRKPNCFELYNPSHKGQVIKACKTEADGRV
  VEGNHVVYRISAPSPEEKEEWMKSIKASISRDPFYDMLATRKRRIANKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163548
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163548.1, NP_001157020.1
• RefSeq Size: 4073 bp
• RefSeq ORF: 1056 bp
• Locus ID: 19159
• Cytogenetics: 5 82.36 cM
• Gene Summary: Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF factors through replacement of GDP with GTP (PubMed:18042453). Play a role in the epithelial polarization (PubMed:20080746).[UniProtKB/Swiss-Prot Function]