Folh1 (NM_001159706) Mouse Tagged ORF Clone

CAT#: MG216035

Folh1 (tGFP-tagged) - Mouse folate hydrolase (Folh1) transcript variant 2, (10ug)

Product Images

Circular map for MG216035

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Folh1
• Synonyms: GCP2; mopsm
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216035 representing NM_001159706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAACGCACTGCAGGACAGAGACTCCGCGGAGGTCCTGGGACACCGCCAGCGCTGGCTCCGTGTTG
  GGACACTGGTGCTGGCTTTAACCGGAACCTTCCTCATTGGCTTCCTCTTTGGGTGGTTTATAAAACCTTC
  CAATGAAGCTACTGGTAATGTTTCCCATTCTGGCATGAAGAAGGAGTTTTTGCATGAATTGAAGGCTGAG
  AACATCAAAAAATTTTTATACAATTTCACACGGACACCACACTTGGCAGGAACACAAAATAATTTTGAGC
  TTGCAAAGCAAATTCATGACCAGTGGAAAGAATTTGGCCTGGATTTGGTTGAGTTATCCCATTACGATGT
  CTTGCTGTCCTATCCAAATAAAACTCATCCTAACTATATCTCAATAATTAATGAAGATGGAAATGAGATT
  TTCAAAACATCATTATCTGAACAGCCACCCCCAGGATATGAGAATATATCAGATGTAGTGCCACCATACA
  GTGCCTTCTCTCCACAAGGGACACCAGAGGGTGATCTAGTGTATGTCAACTATGCACGAACTGAAGACTT
  CTTTAAACTGGAACGGGAAATGAAGATCAGTTGTTCTGGGAAGATTGTGATTGCCAGATATGGGAAAGTG
  TTCAGAGGAAATATGGTTAAAAATGCTCAACTGGCAGGGGCAAAAGGAATGATTCTGTACTCAGACCCTG
  CTGACTACTTTGTTCCTGCGGTGAAGTCCTATCCAGATGGCTGGAACCTCCCTGGAGGTGGTGTCCAACG
  TGGAAATGTCTTAAATCTTAATGGTGCAGGTGACCCGCTCACACCAGGTTACCCAGCAAATGAACATGCT
  TATAGGCATGAGTTGACAAACGCTGTTGGCCTTCCAAGTATTCCTGTCCATCCTATTGGATATGATGATG
  CACAGAAACTCTTAGAAAAGGTCAAGATGCATATTCACTCTTACACTAAAGTGACAAGAATCTATAATGT
  CATTGGCACCCTCAAAGGAGCTCTGGAACCAGACAGATATGTTATTCTTGGAGGTCACCGAGATGCTTGG
  GTATTTGGTGGCATTGACCCTCAGAGTGGAGCAGCTGTTGTTCATGAAATTGTGCGGAGCTTTGGAACCC
  TGAAGAAGAAAGGACGGAGGCCTAGAAGGACAATTTTGTTTGCAAGCTGGGATGCAGAAGAATTTGGCCT
  TCTTGGTTCTACTGAGTGGGCAGAGGAACATTCAAGACTCCTACAAGAGCGAGGTGTGGCTTATATTAAT
  GCTGATTCTTCCATAGAAGGAAATTACACTCTAAGAGTTGATTGCACACCACTGATGTACAGCTTAGTGT
  ACAACCTAACAAAAGAGCTGCAAAGCCCAGATGAAGGTTTTGAAGGAAAATCTCTTTATGACAGCTGGAA
  AGAAAAGAGTCCTTCACCTGAGTTCATTGGAATGCCCAGAATTAGCAAGCTGGGGTCTGGCAATGATTTT
  GAAGTGTTCTTCCAAAGACTTGGAATTGCTTCAGGCAGAGCCCGATATACTAAAAATTGGAAAACTAACA
  AAGTCAGCAGCTATCCTCTCTATCACAGTGTCTATGAAACATATGAGCTGGTAGTAAAATTTTATGACCC
  AACATTTAAATACCACCTCACTGTGGCCCAGGTTCGAGGAGCAATGGTATTTGAACTTGCCAATTCTATA
  GTGCTTCCCTTTGACTGCCAAAGTTATGCTGTAGCTCTGAAGAAGTATGCTGACACTATCTACAATATTT
  CAATGAAACATCCACAAGAAATGAAGGCTTACATGATATCATTTGATTCACTGTTTTCTGCAGTCAATAA
  TTTTACAGATGTTGCATCTAAGTTCAATCAGAGACTGCAAGAGTTAGACAAAAGCAACCCCATATTACTG
  AGAATTATGAATGACCAGCTGATGTATCTGGAACGTGCATTCATTGATCCTTTAGGCTTACCAGGAAGGC
  CTTTCTACAGGCATATCATCTATGCTCCAAGCAGCCACAACAAGTATGCAGGAGAATCATTCCCTGGGAT
  TTATGATGCCCTTTTTGATATAAGTAGCAAAGTCAATGCTTCTAAGGCCTGGAACGAAGTGAAGAGACAG
  ATTTCTATTGCAACCTTTACAGTGCAAGCTGCAGCAGAGACTCTGAGGGAAGTAGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216035 representing NM_001159706
  Red=Cloning site Green=Tags(s)
  
  MWNALQDRDSAEVLGHRQRWLRVGTLVLALTGTFLIGFLFGWFIKPSNEATGNVSHSGMKKEFLHELKAE
  NIKKFLYNFTRTPHLAGTQNNFELAKQIHDQWKEFGLDLVELSHYDVLLSYPNKTHPNYISIINEDGNEI
  FKTSLSEQPPPGYENISDVVPPYSAFSPQGTPEGDLVYVNYARTEDFFKLEREMKISCSGKIVIARYGKV
  FRGNMVKNAQLAGAKGMILYSDPADYFVPAVKSYPDGWNLPGGGVQRGNVLNLNGAGDPLTPGYPANEHA
  YRHELTNAVGLPSIPVHPIGYDDAQKLLEKVKMHIHSYTKVTRIYNVIGTLKGALEPDRYVILGGHRDAW
  VFGGIDPQSGAAVVHEIVRSFGTLKKKGRRPRRTILFASWDAEEFGLLGSTEWAEEHSRLLQERGVAYIN
  ADSSIEGNYTLRVDCTPLMYSLVYNLTKELQSPDEGFEGKSLYDSWKEKSPSPEFIGMPRISKLGSGNDF
  EVFFQRLGIASGRARYTKNWKTNKVSSYPLYHSVYETYELVVKFYDPTFKYHLTVAQVRGAMVFELANSI
  VLPFDCQSYAVALKKYADTIYNISMKHPQEMKAYMISFDSLFSAVNNFTDVASKFNQRLQELDKSNPILL
  RIMNDQLMYLERAFIDPLGLPGRPFYRHIIYAPSSHNKYAGESFPGIYDALFDISSKVNASKAWNEVKRQ
  ISIATFTVQAAAETLREVA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001159706
• ORF Size: 2157 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159706.1, NP_001153178.1
• RefSeq Size: 2948 bp
• RefSeq ORF: 2160 bp
• Locus ID: 53320
• UniProt ID: O35409
• Cytogenetics: 7 D3
• Gene Summary: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.[UniProtKB/Swiss-Prot Function]