Folh1 (NM_001159706) Mouse Untagged Clone

CAT#: MC221006

Folh1 (untagged) - Mouse folate hydrolase (Folh1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Folh1
• Synonyms: GCP2; mopsm
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221006 representing NM_001159706
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGAACGCACTGCAGGACAGAGACTCCGCGGAGGTCCTGGGACACCGCCAGCGCTGGCTCCGTGTTG
  GGACACTGGTGCTGGCTTTAACCGGAACCTTCCTCATTGGCTTCCTCTTTGGGTGGTTTATAAAACCTTC
  CAATGAAGCTACTGGTAATGTTTCCCATTCTGGCATGAAGAAGGAGTTTTTGCATGAATTGAAGGCTGAG
  AACATCAAAAAATTTTTATACAATTTCACACGGACACCACACTTGGCAGGAACACAAAATAATTTTGAGC
  TTGCAAAGCAAATTCATGACCAGTGGAAAGAATTTGGCCTGGATTTGGTTGAGTTATCCCATTACGATGT
  CTTGCTGTCCTATCCAAATAAAACTCATCCTAACTATATCTCAATAATTAATGAAGATGGAAATGAGATT
  TTCAAAACATCATTATCTGAACAGCCACCCCCAGGATATGAGAATATATCAGATGTAGTGCCACCATACA
  GTGCCTTCTCTCCACAAGGGACACCAGAGGGTGATCTAGTGTATGTCAACTATGCACGAACTGAAGACTT
  CTTTAAACTGGAACGGGAAATGAAGATCAGTTGTTCTGGGAAGATTGTGATTGCCAGATATGGGAAAGTG
  TTCAGAGGAAATATGGTTAAAAATGCTCAACTGGCAGGGGCAAAAGGAATGATTCTGTACTCAGACCCTG
  CTGACTACTTTGTTCCTGCGGTGAAGTCCTATCCAGATGGCTGGAACCTCCCTGGAGGTGGTGTCCAACG
  TGGAAATGTCTTAAATCTTAATGGTGCAGGTGACCCGCTCACACCAGGTTACCCAGCAAATGAACATGCT
  TATAGGCATGAGTTGACAAACGCTGTTGGCCTTCCAAGTATTCCTGTCCATCCTATTGGATATGATGATG
  CACAGAAACTCTTAGAAAAGGTCAAGATGCATATTCACTCTTACACTAAAGTGACAAGAATCTATAATGT
  CATTGGCACCCTCAAAGGAGCTCTGGAACCAGACAGATATGTTATTCTTGGAGGTCACCGAGATGCTTGG
  GTATTTGGTGGCATTGACCCTCAGAGTGGAGCAGCTGTTGTTCATGAAATTGTGCGGAGCTTTGGAACCC
  TGAAGAAGAAAGGACGGAGGCCTAGAAGGACAATTTTGTTTGCAAGCTGGGATGCAGAAGAATTTGGCCT
  TCTTGGTTCTACTGAGTGGGCAGAGGAACATTCAAGACTCCTACAAGAGCGAGGTGTGGCTTATATTAAT
  GCTGATTCTTCCATAGAAGGAAATTACACTCTAAGAGTTGATTGCACACCACTGATGTACAGCTTAGTGT
  ACAACCTAACAAAAGAGCTGCAAAGCCCAGATGAAGGTTTTGAAGGAAAATCTCTTTATGACAGCTGGAA
  AGAAAAGAGTCCTTCACCTGAGTTCATTGGAATGCCCAGAATTAGCAAGCTGGGGTCTGGCAATGATTTT
  GAAGTGTTCTTCCAAAGACTTGGAATTGCTTCAGGCAGAGCCCGATATACTAAAAATTGGAAAACTAACA
  AAGTCAGCAGCTATCCTCTCTATCACAGTGTCTATGAAACATATGAGCTGGTAGTAAAATTTTATGACCC
  AACATTTAAATACCACCTCACTGTGGCCCAGGTTCGAGGAGCGATGGTATTTGAACTTGCCAATTCTATA
  GTGCTTCCCTTTGACTGCCAAAGTTATGCTGTAGCTCTGAAGAAGTATGCTGACACTATCTACAATATTT
  CAATGAAACATCCACAAGAAATGAAGGCTTACATGATATCATTTGATTCACTGTTTTCTGCAGTCAATAA
  TTTTACAGATGTTGCATCTAAGTTCAATCAGAGACTGCAAGAGTTAGACAAAAGCAACCCCATATTACTG
  AGAATTATGAATGACCAGCTGATGTATCTGGAACGTGCATTCATTGATCCTTTAGGCTTACCAGGAAGGC
  CTTTCTACAGGCATATCATCTATGCTCCAAGCAGCCACAACAAGTATGCAGGAGAATCATTCCCTGGGAT
  TTATGATGCCCTTTTTGATATAAGTAGCAAAGTCAATGCTTCTAAGGCCTGGAACGAAGTGAAGAGACAG
  ATTTCTATTGCAACCTTTACAGTGCAAGCTGCAGCAGAGACTCTGAGGGAAGTAGCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001159706
• Insert Size: 2160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001159706.1, NP_001153178.1
• RefSeq Size: 2948 bp
• RefSeq ORF: 2160 bp
• Locus ID: 53320
• UniProt ID: O35409
• Cytogenetics: 7 D3
• Gene Summary: Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1.