0610009O20Rik (NM_024179) Mouse Tagged ORF Clone

CAT#: MG215896

0610009O20Rik (tGFP-tagged) - Mouse RIKEN cDNA 0610009O20 gene (0610009O20Rik), (10ug)

Product Images

Circular map for MG215896

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 0610009O20Rik
• Synonyms: 2700004E22Rik; Dele; Kiaa0141
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215896 representing NM_024179
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCGCCTGACAGGGATCCTGGGGCGAGCTCTTCCCCGCCTGCTGGGACCTGGCTTCCGTGGGATAA
  CGCCCAAACCCACCAGTTCAGATGGGTCTCAGACAACCTCCCCTACCCTGCCGCTCACTAGGCTCAGCTT
  TGACAGATCAGGTTCCCACGGTTCAAAGAGGAGCAGGGACCCAAAGTGCTGTGGATGGAAAGATGCCTTT
  CACTGGATGTCTGCTCATGTCTCCCCAAACACACTGCGGGACGCCATCTCATGGGGCACTCTGGCGGTGC
  TGGCCTTGCACCTGGCGAGGCAGATCCACTTCCACGCCCCTCTGGTAGCAGGACCTCAGCCAGCTGAACG
  CTCTTGGCACAGTCCCCTGTACCGCTTCCTCTCCTCTTCCTGGTGGCACCCACACTCCTCACTTCGGAGG
  CATGTTCTTCCCAGGTCTGACTGCCCAGCTCCCAGGAACACTGGCCTCAGGGAACCAAGGCAGGGCCAGG
  AAGACCATCCCTCAGCTCCATCCCAGTGCCTTCCCTCAGATAGCTCCCTGAGATCTGGACTTCTGAATCT
  GCCTGAAGAAGAGCCCAGTGACTTTGACTTCCTGCATGCTAGCCGAGACTTCGCATCCCAGGCAAAGGCA
  GCCGAGGCCCACCCCCCTGGAGGAAAAAATGAACAAGACAAGGCCAAGGCTCTTCCTCTGGAGGAGGCTG
  TGACTTCCATTCAGCAACTCTTTCAGCTCAGTGTAGCCATCACTTTCAACTTCCTGGGGACAGAGAACAT
  AAAGACGGGGGACTACACGGCAGCCTTTTCCTACTTCCAGAAAGCCGCAGACCGTGGCTACAGCAAAGCA
  CAGTACAATGTGGGCTTGTGTCTCGAGCATGGCAGAGGCACCCCCAGGGACCTCAGCAAGGCTATCCTCT
  TTTACCATCTGGCTGCCGTCCAGGGCCACAGCCTGGCTCAGTACCGCTATGCCAGGTGTCTGTTGCAAAG
  TCCAGGCTCTTTGTCAGACCCTGAGCGAGAGAGGGCAGTCTCTCTGCTGAAGCAGGCTGCAGACTCTGGC
  TTGACAGAGGCCCAAGCTTTCCTTGGGGTGCTCTTTACTAAGGAACCACACCTGGATGAACAGAGAGCAG
  TAAAATACCTCTGGCTGGCGGCCAGCAATGGGGATTCTCAGAGCAGATTCCACTTGGGAATTTGCTATGA
  GAAGGGCCTTGGTGCACAGAGGAATCTAGGAGAGGCCGTGAAGTGCTACCAGCAGGCAGCAGCTATGGGG
  AATGAACCCGCCCGGGAGAGGCTTCGGACCCTCTTTAACGTGGAAGCAGCAGGGCCCAGCCACCTGGCCA
  CGACAGGACTGAAGTCTTTCTCCAGCCCCTCCCTCTGCAGCCTGAACACCCTGTTGGCTGGTGCCTCAGG
  CCTCCCTCATGCCTCAAGCACTGGGAACCTCGGCCTCCTCTGCAGAAGTGGCCATCTCGGAGCCAGCCAT
  GGTGCCCCCAGCAGGACTATACCATCCTTGGAAAGGAGCCTCGTAAGACTAGGTTTCGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215896 representing NM_024179
  Red=Cloning site Green=Tags(s)
  
  MWRLTGILGRALPRLLGPGFRGITPKPTSSDGSQTTSPTLPLTRLSFDRSGSHGSKRSRDPKCCGWKDAF
  HWMSAHVSPNTLRDAISWGTLAVLALHLARQIHFHAPLVAGPQPAERSWHSPLYRFLSSSWWHPHSSLRR
  HVLPRSDCPAPRNTGLREPRQGQEDHPSAPSQCLPSDSSLRSGLLNLPEEEPSDFDFLHASRDFASQAKA
  AEAHPPGGKNEQDKAKALPLEEAVTSIQQLFQLSVAITFNFLGTENIKTGDYTAAFSYFQKAADRGYSKA
  QYNVGLCLEHGRGTPRDLSKAILFYHLAAVQGHSLAQYRYARCLLQSPGSLSDPERERAVSLLKQAADSG
  LTEAQAFLGVLFTKEPHLDEQRAVKYLWLAASNGDSQSRFHLGICYEKGLGAQRNLGEAVKCYQQAAAMG
  NEPARERLRTLFNVEAAGPSHLATTGLKSFSSPSLCSLNTLLAGASGLPHASSTGNLGLLCRSGHLGASH
  GAPSRTIPSLERSLVRLGFG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024179
• ORF Size: 1530 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024179.5, NP_077141.2
• RefSeq Size: 2407 bp
• RefSeq ORF: 1533 bp
• Locus ID: 66839
• UniProt ID: Q9DCV6
• Cytogenetics: 18 B3
• Gene Summary: Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation.[UniProtKB/Swiss-Prot Function]