Sqor (NM_001162503) Mouse Tagged ORF Clone

CAT#: MG215895

Sqrdl (tGFP-tagged) - Mouse sulfide quinone reductase-like (yeast) (Sqrdl) nuclear gene encoding mitochondrial protein transcript variant 2, (10ug)

Product Images

Circular map for MG215895

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sqor
• Synonyms: 0610039J17Rik; 4930557M22Rik; SQOR; Sqrdl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215895 representing NM_001162503
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCACTGGTCGCTGTTGTCTCCAGTCCACGTGCCCGGCTCTTTGCCTGTTTCCTTAGGCTGGGCA
  CTCAGCAGGCTGGACCCCTCCAATTGCATACAGGAGCCTGCTGCACAGCCAAGAACCACTATGAGGTGCT
  GGTACTTGGTGGGGGAGCTGGGGGGATCACCATGGCCACTCGAATGAAGAGGAGAGTTGGAGCAGAGAAT
  GTGGCCATTGTTGAACCCAGTGAGAGGCACTTCTACCAGCCAATCTGGACACTAGTGGGCGCGGGTGCCA
  AAGAGTTGTCCTTGTCAGTCCGTTCCACACTGAGTGTGATTCCATCCGGAGTGCAGTGGATTCAAGATAG
  AGTGGCGGAACTGAACCCAGACGAGAACTGTATCCGCACAGACAGTGGCAAGGAGATCTCCTACAGATAC
  CTTATCATCGCTCTTGGAATCCAGCTGGACTATGAGAAGATTAAAGGACTACCTGAAGGCTTTGCTTATC
  CCAAAATAGGGTCCAATTACTCAGTGAAGACAGTGGAGAAGACATGGAAGGCCCTGCAGGATTTCAAGGA
  AGGCAATGCCCTTTTCACCTTCCCAAACACTCCGGTGAAGTGTGCTGGTGCCCCCCAGAAGATCATGTAC
  CTATCAGAAGCCTACTTCAGGAAGACTGGAAAGCGACCCAAGGCAAACATCATCTTCAACACAGCTTTAG
  GAACAATTTTTGGAGTTAAGAAGTATGCAGATGCCTTGCAGGAGATCATTCGGGAGAGGGACGTCTCTGT
  CAACTATAAGCACAACCTTATTGAGGTCCGACCTGACAAACAGGAGGCCGTTTTTGAGATCCTGGACAAG
  CCTGGGGAGACGCATGTGATTCCTTATGAAATGCTTCATGTCACACCACCTATGAGTGCACCAGATGTCC
  TCAAGAGGAGTCCTGTGGCTGATTCTGCTGGCTGGGTGGACGTGGACAAAGAGACTCTGCAGCATAAGAA
  ATACCCGAATGTGTTTGGCATTGGGGACTGCACCAATCTTCCAACTTCAAAGACTGCTGCTGCAGTAGCT
  GCCCAGTCTGGAATCCTTGATAGAACAATGTGTCTGATTATGAAGAACCAAAGACCCATAAAAAAGTATG
  ATGGTTATACCTCGTGCCCACTGGTAACTGGCTACAACCGAGTGATTCTTGCTGAGTTTGACTACACAGC
  TCAGCCCCTGGAAACCTTCCCTTTTGATCAGAGTAAAGAGCGAATTACTATGTACCTAATGAAGGCTGAC
  ATGATGCCATTCCTGTACTGGAATATGATGCTCAGAGGCTACTGGGGAGGTCCAGCCTTCTTGCGAAAGC
  TGTTTCATCTGGGCATGAAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215895 representing NM_001162503
  Red=Cloning site Green=Tags(s)
  
  MAPLVAVVSSPRARLFACFLRLGTQQAGPLQLHTGACCTAKNHYEVLVLGGGAGGITMATRMKRRVGAEN
  VAIVEPSERHFYQPIWTLVGAGAKELSLSVRSTLSVIPSGVQWIQDRVAELNPDENCIRTDSGKEISYRY
  LIIALGIQLDYEKIKGLPEGFAYPKIGSNYSVKTVEKTWKALQDFKEGNALFTFPNTPVKCAGAPQKIMY
  LSEAYFRKTGKRPKANIIFNTALGTIFGVKKYADALQEIIRERDVSVNYKHNLIEVRPDKQEAVFEILDK
  PGETHVIPYEMLHVTPPMSAPDVLKRSPVADSAGWVDVDKETLQHKKYPNVFGIGDCTNLPTSKTAAAVA
  AQSGILDRTMCLIMKNQRPIKKYDGYTSCPLVTGYNRVILAEFDYTAQPLETFPFDQSKERITMYLMKAD
  MMPFLYWNMMLRGYWGGPAFLRKLFHLGMN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162503
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162503.1, NP_001155975.1
• RefSeq Size: 1676 bp
• RefSeq ORF: 1353 bp
• Locus ID: 59010
• UniProt ID: Q9R112
• Cytogenetics: 2 E5
• Gene Summary: Catalyzes the oxidation of hydrogen sulfide with the help of a quinone, such as ubiquinone, giving rise to thiosulfate and ultimately to sulfane (molecular sulfur) atoms. Requires an additional electron acceptor; can use sulfite, sulfide or cyanide (in vitro).[UniProtKB/Swiss-Prot Function]