Psma8 (NM_001163609) Mouse Tagged ORF Clone

CAT#: MG215785

Psma8 (tGFP-tagged) - Mouse proteasome (prosome macropain) subunit alpha type 8 (Psma8), (10ug)

Product Images

Circular map for MG215785

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Psma8
• Synonyms: 2410072D24Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215785 representing NM_001163609
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTCGGTATGACAGGGCAATCACCGTCTTCTCCCCAGATGGACACCTTTTCCAAGTGGAGTATG
  CCCAGGAAGCAGTGAAGAAAGGCTCCACCGCGGTTGGAATTCGAGGAACTAATATAGTTGTGCTTGGGGT
  AGAAAAAAAGTCAGTGGCCAAGCTTCAAGATGAAAGGACTGTGAGGAAAATCTGCGCTCTCGATGACCAT
  GTCTGCATGGCTTTTGCAGGTCTGACAGCGGATGCTAGAGTGGTGATCAGCAGAGCGCGGGTGGAGTGCC
  AGAGCCACAAGCTCACAGTGGAGGATCCCGTCACTGTAGAGTATATAACTCGCTTCATAGCGACGCTAAA
  GCAGAAATATACCCAGAGCAATGGACGAAGGCCTTTTGGTATTTCAGCCTTAATTGTAGGCTTTGATGAT
  GACGGCATCCCAAGATTGTATCAGACAGATCCCTCCGGGACTTACCACGCTTGGAAAGCAAATGCAATAG
  GCCGAAGTGCTAAAACTGTCCGGGAATTTCTGGAAAAAAATTACACAGAAGATGCAATTTCAAATGACAA
  GGAAGCTATCAAATTAGCAATAAAAGCTTTGCTCGAAGTTGTCCAGTCTGGCGGAAAGAACATTGAACTG
  GCTATAATAAGAAGAGATCAACCTTTGAAGATGTTTAGTGCCAAAGAAATTGAACTGGAGGTGAGTGAAA
  TAGAAAGGGAAAAGGACGAAGCAGAGAAGACAAAGTCAAAGAAGTCAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215785 representing NM_001163609
  Red=Cloning site Green=Tags(s)
  
  MASRYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGIRGTNIVVLGVEKKSVAKLQDERTVRKICALDDH
  VCMAFAGLTADARVVISRARVECQSHKLTVEDPVTVEYITRFIATLKQKYTQSNGRRPFGISALIVGFDD
  DGIPRLYQTDPSGTYHAWKANAIGRSAKTVREFLEKNYTEDAISNDKEAIKLAIKALLEVVQSGGKNIEL
  AIIRRDQPLKMFSAKEIELEVSEIEREKDEAEKTKSKKST
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163609
• ORF Size: 750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163609.1, NP_001157081.1
• RefSeq Size: 1693 bp
• RefSeq ORF: 753 bp
• Locus ID: 73677
• UniProt ID: Q9CWH6
• Cytogenetics: 18 A1
• Gene Summary: Component of the spermatoproteasome, a form of the proteasome specifically found in testis that promotes degradation of histones, thereby participating actively to the exchange of histones during spermatogenesis. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.[UniProtKB/Swiss-Prot Function]