Duox1 (NM_001099297) Mouse Tagged ORF Clone

CAT#: MG215591

Duox1 (tGFP-tagged) - Mouse dual oxidase 1 (Duox1), (10ug)

Product Images

Circular map for MG215591

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Duox1
• Synonyms: 9930101G15Rik; AW987690; Duox2; LNOX1; LNOX2; NOXEF1; NOXEF2; P138-TOX; THOX1; THOX2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215591 representing NM_001099297
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTCCACTTAGCTCTGGCCTGGATTCTCCTGGTTGGGACACTGGCTTCTTTGGGAGCTCAGAACT
  CCATTTCTTGGGAGGTACAGCGATTTGATGGATGGTATAACAACCTCATGGAGCACAGATGGGGCAGCAA
  AGGCTCTCGGCTGCAGCGCCTTGTTCCAGCCAGCTATGCAGATGGTGTCTACCAGCCCCTGAAAGAACCC
  TACCTGCCTAACCCCCGACACCTTAGTAACAGGGTCATGAGAGGTTCTGCAGGGCAGCCATCTCTTCGGA
  ACCGTACAGTCCTGGGTGTCTTCTTTGGCTACCACGTGCTCTCAGACCTGGTGAGTGTGGAAACACCAGG
  CTGCCCCGCAGAGTTCCTCAACATTTACATCCCGCATGGGGACCCGGTGTTCGACCCTGACAAGCGCGGG
  AACGTGGTGCTGCCCTTTCAAAGGAGCCGCTGGGACCGCAACACTGGACAGAGCCCCAGCAACCCCCGGG
  ACCAGAGCAACCAGGTGACCGGCTGGCTAGATGGCAGCGCCATCTATGGCTCCTCTCATTCCTGGAGTGA
  CACTCTGAGGAGCTTCTCTGGAGGACAGCTGGCTTCTGGGCCTGACCCTGCTTTCCCATCGGACTCCCAG
  AGCTCTCTGCTCATGTGGATGGCGCCGGACCCCTCCACAGGGCAGGGAGGGCCACGGGGGGTGTATGCCT
  TCGGGGCCCAGCGCGGGAACAGGGAGCCCTTTCTGCAGGCTCTGGGCTTGTTGTGGTTTCGCTACCACAA
  CCTGTGTGCCAGGAAGCTGGCGCAGGAGCACCCGCACTGGGGGGATGAGGAACTGTTCCAGCATGCTCGC
  AAGAGGGTCATTGCCACCTACCAGAACATTGCGATGTATGAGTGGCTGCCCAGCTTCCTGAAGCAAACTC
  CTCCGGAGTATCCAGGATACCGTCCATTTCTGGACCCCAGTATCTCCCCAGAGTTCGTTGTGGCCTCTGA
  GCAGTTCCTCTCTACCATGGTGCCCTCTGGGGTCTACATGAGGAATGCCAGTTGTCATTTCCAGGGGATT
  CCCAGTCATAACTCAAGTGTCTCTGGAGCTCTCCGGGTCTGCAACAGCTACTGGAGTCGAGAGCACCCCA
  AGCTACAAAGGGCTGAAGATGTGGATGCACTGCTGCTGGGCATGGCCTCCCAGATTGCTGAGAGAGAGGA
  CCACGTGGTGGTTGAGGACATGCAAGATTTCTGGCCTGGGCCACTGAAGTTTTCCCGTACAGACTACCTG
  GCCAGCTGCCTGCAGAGGGGCCGGGATCTAGGCCTGCCTTCTTACACCAAAGCCAGGGAGGCACTGGGCC
  TGTCTCCCATTTCCCACTGGCAGGACATCAACCCTGCACTCTCCAGGAGCAATGGCACTGTGCTGGAGGC
  CACAGCTGCCCTGTACAATCAGGACCTGTCTCGGCTGGAGCTACTCCCAGGGGGGCTTCTGGAGAGCCAT
  GGAGACCCTGGACCCCTTTTCAGCACTATTGTCCTTGACCAGTTTGTCAGGCTACGAGATGGTGACCGTT
  ACTGGTTTGAAAACACCAGGAACGGATTGTTCTCTAAGGAAGAGATCGCAGAGATCCGAAACACTTCCCT
  ACGGGACATTCTAGTGGCTGTCACCAATGTGGACCCCAGTGCCCTACAGCCCAATGTCTTCTTTTGGCTT
  GCAGGAGACCCCTGTCCACAGCCAAGTCAGCTCAGCGCCAAGGGCCTGCCAGCCTGTGCCCCCCTCTTCA
  TTCGGGACTATTTCGAGGGGAGTGGATTTGGCTTCGGACTCACCATTGGGACCCTTTGCTGTTTCCCCTT
  AGTCAGCCTCCTTAGTGCCTGGATTGTTGCCCGGCTTCGAAAGAGGAATTTCAAGAGGCTCCAGAGACAA
  GACCGCCAGAGCATCATGTCGGAGAAGCTTGTAGGGGGAGTGGAAGCTTTGGAGTGGCAGGGCCGCAATG
  AACCCTGCAGGCCAGTGCTTGTGCACTTACAGCCGGGACAGATTCGAGTAGTGGATGGCAGGCTCACCGT
  GCTCCGGACCATCCAGCTGCGACCCCCACAGCAGGTCAACCTCATCCTGTCCAGCAACCGTGGACGCCGC
  ACCCTGCTGCTCAAGATCCCCAAGGAGTATGACCTGGTTCTGTTGTTTAACATGGAGGAGGAACGGCAGG
  CACTGGTGGAAAACGTCAGGGGAGCTCTGAAGGAAAATGGGCTGAGCTTCCAGGAGTGGGAACTGAGGGA
  GCAGGAGCTGATGAGGGCAGCAGTGACCAGGCAACAACGGGGCCACCTCCTGGAGACCTTCTTCAGGCAC
  CTTTTCTCCCAGGTGTTAGACATCAATCAGGCTGATGCAGGGACTCTGCCTCTGGATTCCTCGACCAAAG
  TGCGTGAGGCCCTGACCTGTGAGCTGAGCAGAGCTGAGTTTGCAGACTCCCTAGGCCTCAAGCCCCAGGA
  CATGTTTGTGGAGTCCATGTTTTCTCTAGCCGACAAGGATGGCAATGGCTACCTGTCCTTCCGGGAGTTC
  CTGGACATCCTGGTGGTCTTCATGAAAGGCTCCCCAGAGGAGAAGTCTCGTCTTATGTTCCGAATGTATG
  ACTTTGATGGGAACGGTCTCATTTCCAAGGATGAGTTCATTAGGATGCTAAGGTCCTTCATCGAGATCTC
  CAACAACTGCCTGTCCAAGGCCCAGCTGGCTGAGGTGGTTGAGTCTATGTTCCGGGAGTCTGGCTTCCAG
  GACAAGGAGGAGCTGACCTGGGAGGACTTCCACTTCATGCTGCGGGACCACGACAGTGATCTCCGATTCA
  CACAGCTCTGTGTCAAAGGTGTGGAAGTGCCGGAAGTGATCAAGAACCTCTGCCGGCGAGCCTCCTACAT
  CAGCCAGGAGAAGATCTGCCCTTCGCCCAGAATGAGCGCCCACTGTGCCCGAAACAACATGAAGACTGCG
  TCATCACCACAGAGACTGCAGTGCCCCATGGACACAGACCCTCCTCAAGAGATTCGACGGAGGTTTGGCA
  AAAAGGTAACTTCATTCCAGCCCTTACTGTTCACTGAGGCACACCGAGAGAAGTTTCAACGCAGCCGCCG
  CCACCAAACGGTGCAGCAGTTCAAGCGCTTTATTGAGAATTACAGGCGCCACATTGGCTGTGTGGCGGTG
  TTTTACACCATCACTGGGGCGCTCTTCCTGGAGAGAGCCTACTACTATGCATTTGCAGCACACCACAGCG
  GCATCACCGATACCACCCGTGTAGGCATCATCCTGTCCCGGGGCACGGCGGCCAGCATCTCCTTTATGTT
  TTCCTACATCCTGCTCACCATGTGTCGCAACCTCATCACCTTCTTGCGGGAGACCTTCCTCAACCGCTAC
  ATTCCCTTCGATGCTGCCGTGGACTTCCATCGCCTCATCGCCTCCACGGCCATCATCCTCACAGTCTTAC
  ACAGTGCTGGCCACGTAGTGAATGTGTACCTGTTCTCCATCAGTCCACTCAGTGTCCTCTCTTGCCTCTT
  CCCTGGCCTCTTCCACGATGATGGGTCGGAGTTCCCTCAGAAGTATTACTGGTGGTTCTTCCAGACTGTG
  CCAGGTCTCACTGGGGTCCTCCTGCTCCTGGCCCTGGCCATCATGTACGTCTTCGCCTCCCACCACTTCC
  GCCGCCGCAGTTTCCGGGGCTTCTGGCTGACCCATCACCTCTACATTTTTCTCTACATTCTGCTCATCAT
  ACATGGCAGCTTTGCTCTGATCCAGATGCCCCGGTTCCACATCTTCTTCCTGGTCCCTGCAATTATTTAT
  GTGGGGGACAAGCTGGTGAGCCTGAGCCGGAAGAAGGTGGAGATCAGCGTGGTGAAGGCTGAGCTGCTGC
  CATCAGGAGTGACCCACCTGCGGTTCCAGAGGCCTCAGGGCTTTGAGTATAAGTCAGGGCAGTGGGTTCG
  GATTGCGTGCCTGGCTCTGGGAACCACCGAGTATCACCCTTTCACACTGACTTCTGCACCCCACGAGGAC
  ACACTCAGCCTGCACATCCGGGCGGCTGGACCCTGGACCACTCGCCTCAGGGAAATCTACTCACCCCCAA
  CGGGTGACACCTGTGCCAGATACCCAAAGCTGTACCTCGATGGACCGTTTGGAGAGGGCCACCAGGAGTG
  GCACAAGTTTGAGGTGTCAGTGCTGGTAGGAGGGGGCATTGGAGTCACTCCCTTTGCCTCCATCCTCAAA
  GACCTGGTTTTCAAGTCATCAGTCAGCTGCCAAGTGTTCTGTAAGAAGATCTACTTCATCTGGGTGACAA
  GGACTCAGAGGCAGTTTGAGTGGCTGGCTGACATCATCCGGGAGGTGGAGGAGAATGACCGCCAGGACCT
  GGTGTCTGTGCACATCTATATTACTCAGCTGGCTGAGAAGTTCGACCTCAGGACCACCATGCTGTACATC
  TGTGAGCGGCACTTCCAGAAGGTGCTGAACAGGAGTCTGTTCACGGGCCTGCGCTCCATCACCCACTTTG
  GTCGCCCTCCCTTTGAGCCCTTTTTCAACTCTCTGCAGGAAGTCCACCCCCAAGTCCGAAAGATTGGAGT
  ATTTAGCTGTGGTCCCCCTGGCATGACCAAGAATGTGGAGAAGGCTTGTCAGCTCATCAACAGGCAGGAC
  AGGACTCATTTTTCTCACCATTATGAGAACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215591 representing NM_001099297
  Red=Cloning site Green=Tags(s)
  
  MGFHLALAWILLVGTLASLGAQNSISWEVQRFDGWYNNLMEHRWGSKGSRLQRLVPASYADGVYQPLKEP
  YLPNPRHLSNRVMRGSAGQPSLRNRTVLGVFFGYHVLSDLVSVETPGCPAEFLNIYIPHGDPVFDPDKRG
  NVVLPFQRSRWDRNTGQSPSNPRDQSNQVTGWLDGSAIYGSSHSWSDTLRSFSGGQLASGPDPAFPSDSQ
  SSLLMWMAPDPSTGQGGPRGVYAFGAQRGNREPFLQALGLLWFRYHNLCARKLAQEHPHWGDEELFQHAR
  KRVIATYQNIAMYEWLPSFLKQTPPEYPGYRPFLDPSISPEFVVASEQFLSTMVPSGVYMRNASCHFQGI
  PSHNSSVSGALRVCNSYWSREHPKLQRAEDVDALLLGMASQIAEREDHVVVEDMQDFWPGPLKFSRTDYL
  ASCLQRGRDLGLPSYTKAREALGLSPISHWQDINPALSRSNGTVLEATAALYNQDLSRLELLPGGLLESH
  GDPGPLFSTIVLDQFVRLRDGDRYWFENTRNGLFSKEEIAEIRNTSLRDILVAVTNVDPSALQPNVFFWL
  AGDPCPQPSQLSAKGLPACAPLFIRDYFEGSGFGFGLTIGTLCCFPLVSLLSAWIVARLRKRNFKRLQRQ
  DRQSIMSEKLVGGVEALEWQGRNEPCRPVLVHLQPGQIRVVDGRLTVLRTIQLRPPQQVNLILSSNRGRR
  TLLLKIPKEYDLVLLFNMEEERQALVENVRGALKENGLSFQEWELREQELMRAAVTRQQRGHLLETFFRH
  LFSQVLDINQADAGTLPLDSSTKVREALTCELSRAEFADSLGLKPQDMFVESMFSLADKDGNGYLSFREF
  LDILVVFMKGSPEEKSRLMFRMYDFDGNGLISKDEFIRMLRSFIEISNNCLSKAQLAEVVESMFRESGFQ
  DKEELTWEDFHFMLRDHDSDLRFTQLCVKGVEVPEVIKNLCRRASYISQEKICPSPRMSAHCARNNMKTA
  SSPQRLQCPMDTDPPQEIRRRFGKKVTSFQPLLFTEAHREKFQRSRRHQTVQQFKRFIENYRRHIGCVAV
  FYTITGALFLERAYYYAFAAHHSGITDTTRVGIILSRGTAASISFMFSYILLTMCRNLITFLRETFLNRY
  IPFDAAVDFHRLIASTAIILTVLHSAGHVVNVYLFSISPLSVLSCLFPGLFHDDGSEFPQKYYWWFFQTV
  PGLTGVLLLLALAIMYVFASHHFRRRSFRGFWLTHHLYIFLYILLIIHGSFALIQMPRFHIFFLVPAIIY
  VGDKLVSLSRKKVEISVVKAELLPSGVTHLRFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHED
  TLSLHIRAAGPWTTRLREIYSPPTGDTCARYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILK
  DLVFKSSVSCQVFCKKIYFIWVTRTQRQFEWLADIIREVEENDRQDLVSVHIYITQLAEKFDLRTTMLYI
  CERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLINRQD
  RTHFSHHYENF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099297
• ORF Size: 4653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099297.1, NP_001092767.1
• RefSeq Size: 5267 bp
• RefSeq ORF: 4656 bp
• Locus ID: 99439
• Cytogenetics: 2 E5