Olfr463 (NM_146413) Mouse Tagged ORF Clone

CAT#: MG215520

Olfr463 (tGFP-tagged) - Mouse olfactory receptor 463 (Olfr463), (10ug)

Product Images

Circular map for MG215520

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Olfr463
• Synonyms: MOR240-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215520 representing NM_146413
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACCAGGGAACCTCACATGGGTGTCAGAGTTTGTCTTCCTGGGGTTCTCAGAGATCTGGGAGCTTC
  AGGTCTTCCTGTTTGTGGTGTTCTTGTGTGTCTACAGCACGACCGTGGTGGGAAACCTCCTCATCATAGT
  TACGGTTTCCTCAGACCCCAGGCTCCACACACCTATGTACTTCCTGCTCCGCAACCTGGCTGTCTTAGAC
  CTCTGCTTCTCCTCAGTCACTGCCCCCAAGATGCTGGTGGACTTTCTCTCTGAGAAGAAGACTATCTCCT
  ACCGAGGCTGCATGGTACAGATCTTCTTCTTCCACTTCCTGGGAGGGGCCATGGTCTTCTTCCTCTCAGT
  AATGGCTTATGACCGCCTGGTGGCTATTTCCCGACCTCTCCACTATGTCACCATCATGAACAGCCAGCTT
  TGTATGGGGCTGGTGGTGGCTTCCTGGGTAGGAGGCTTTGCCCACTCGATTGTCCAGCTGTCTCTGATGC
  TCCCGTTGCCGTTCTGTGGACCCAATGTCCTGGATAACTTCTACTGTGATGTCCCCCAAGTACTGAGACT
  TGCTTGCATGGACACCTCCCTCTTGGAGTTCCTCATGATCTCCAACAGTGGGATGCTGGATGTCATCTGG
  TTCTTTCTCCTTCTCATCTCCTACTTGGTCATCCTGGTGATGCTGAGGTCTCACTCAGGGGAGGCGAGGA
  GGAAGGCGGCGTCCACCTGCACCACCCACATCATCGTGGTGTCTATGATCTTCATTCCAAGCATTTACCT
  GTATGCCCGGCCCTTCACCCCCTTCACCATGGACAAGGCTGTGTCCATCAGCCATACGGTCATGACCCCC
  ATGCTCAACCCCATGATCTACACGCTCAGGAACCAGGAGATGCAGGCAGCCATGAAGAGACTAGCAAAGC
  GCTTAGCGCTTTGCAACAGGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215520 representing NM_146413
  Red=Cloning site Green=Tags(s)
  
  MEPGNLTWVSEFVFLGFSEIWELQVFLFVVFLCVYSTTVVGNLLIIVTVSSDPRLHTPMYFLLRNLAVLD
  LCFSSVTAPKMLVDFLSEKKTISYRGCMVQIFFFHFLGGAMVFFLSVMAYDRLVAISRPLHYVTIMNSQL
  CMGLVVASWVGGFAHSIVQLSLMLPLPFCGPNVLDNFYCDVPQVLRLACMDTSLLEFLMISNSGMLDVIW
  FFLLLISYLVILVMLRSHSGEARRKAASTCTTHIIVVSMIFIPSIYLYARPFTPFTMDKAVSISHTVMTP
  MLNPMIYTLRNQEMQAAMKRLAKRLALCNRE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146413
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146413.2, NP_666525.2
• RefSeq Size: 936 bp
• RefSeq ORF: 936 bp
• Locus ID: 258408
• Cytogenetics: 11 C
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]