Fbxw15 (NM_199036) Mouse Tagged ORF Clone

CAT#: MG215379

Fbxw15 (tGFP-tagged) - Mouse F-box and WD-40 domain protein 15 (Fbxw15), (10ug)

Product Images

Circular map for MG215379

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fbxw15
• Synonyms: Fbxo12J
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215379 representing NM_199036
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATCCATTTACCTTGTTTGCCGATGATGAAAATCCTCTCCTATCTGGATGCCTACAGTTTGCTAC
  AGGCTGCCCAAGTGAACAAGGACTGGAATGAACTTGCCAGCAGTGATGTCCTGTGGAGGAAATTGTGTCA
  GAAGAGATGGCTCTACTGTGACATGGATACCCTGCAGCTCCAGGGCAAAGAGACATGGAAGCAGTTCTTC
  ATTGATCGAATATGGCAAGAACGTGCCAAGTTCCGTGCAAAGGCAAAAGATTTCACTTACAAGGAAATTC
  CTTTGATGTGTGGACTTTTTGGATATGCATGTTATATCTCAGGGTGTGGCTTAACAAGAAAGGGACAAGA
  CAAGTCTGTTGTCTGTATGGTGAATTCCAAGAATACAATTTCCACCTGGGATGTTCACAAGAGCGTTATA
  ACCTGGAAAAGCCCAGAACAGCCGGCAAGTATCAAGCTGTTGACTACCCTCCCTGAGATGCACATTGCAG
  TCACTGTAGATATTCAATCAACTATCAAACTGTGGGACTGTCACAACAGGGAAGCTCTGGCGACTAACAA
  CCTGAAGTCTCCATGCAAATCACTGAAAGCTGTCTTTACCAAGGATGGCCCAATTGTCTTGATAGGTGAT
  ACCTTGGGTAACATCCACATCTTTAGGATTCCAGATTTATACCTCATTTCCACAGTCAATGTATTGCCAT
  ATGGTTTTGATGGAATCTACTGTTCTCCTCAGAAGAAATGGGTCTTACTGAGTAAGAAACACCCACATAT
  TTTGCCAAAGGTGTTTTACATGAGCAGCTTTCTGAGGACATCAGAATTCTCTGCCCCTGTGTCTACCGTT
  CTCAAATTATCCTTATATGAAAGAGTCTTCTGGACACCAAGAAGGGAAGACAGGATAACCCTGATGTCCA
  GAAGTGGTTTCCCCCAAGTCAAAATGTTTGAAACATATGATATCAAGCTGGAAGAGTTTGGGAACAAAAG
  AATTGTTAAAGGAAAATTGATAGCAAGCTTCGAGTTACAATGTCACAAGGTGAACCCACAGAGGTTTGGA
  GTCAGTGATAAGAACGTGATTGTTTGTTCAACTGAATCCTCTCTCTTGCTCTTCGACATAAATGGTCTCC
  GTCTAAAGACATTTCAGTACTGCCCAGAAATGATCGTGAAACTCAGTGTGGACCCCCTTCATGTCATTGT
  CATTTGTAACACTGGCTCTATGGATGTGTATGCATGGGAGGAGAGAAGCCTGCTGCTCAGGAAGTGTTAT
  CGACTGCACATTGAGAGACCTCTGCCACTGTACGGCTTCATTTATAAAGCAGCATGTGATGATGTGAGCA
  TAATTCAGTTGATTACAGATGAGCTCAGCCTCAGCTCTCTGACTTCATATGCCTTGAACATCTGCTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215379 representing NM_199036
  Red=Cloning site Green=Tags(s)
  
  MAIHLPCLPMMKILSYLDAYSLLQAAQVNKDWNELASSDVLWRKLCQKRWLYCDMDTLQLQGKETWKQFF
  IDRIWQERAKFRAKAKDFTYKEIPLMCGLFGYACYISGCGLTRKGQDKSVVCMVNSKNTISTWDVHKSVI
  TWKSPEQPASIKLLTTLPEMHIAVTVDIQSTIKLWDCHNREALATNNLKSPCKSLKAVFTKDGPIVLIGD
  TLGNIHIFRIPDLYLISTVNVLPYGFDGIYCSPQKKWVLLSKKHPHILPKVFYMSSFLRTSEFSAPVSTV
  LKLSLYERVFWTPRREDRITLMSRSGFPQVKMFETYDIKLEEFGNKRIVKGKLIASFELQCHKVNPQRFG
  VSDKNVIVCSTESSLLLFDINGLRLKTFQYCPEMIVKLSVDPLHVIVICNTGSMDVYAWEERSLLLRKCY
  RLHIERPLPLYGFIYKAACDDVSIIQLITDELSLSSLTSYALNICS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_199036
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_199036.2, NP_950201.2
• RefSeq Size: 1520 bp
• RefSeq ORF: 1401 bp
• Locus ID: 382105
• UniProt ID: L7N1X6
• Cytogenetics: 9 F2
• Gene Summary: Substrate-recognition component of an SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Promotes KAT7 ubiquitination and subsequent degradation in collaboration with MAP2K1 kinase, leading to reduced histone H3K14 acetylation and increased cell proliferation.[UniProtKB/Swiss-Prot Function]