Dgkd (NM_177646) Mouse Tagged ORF Clone

CAT#: MG215309

Dgkd (tGFP-tagged) - Mouse diacylglycerol kinase delta (Dgkd), (10ug)

Product Images

Circular map for MG215309

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dgkd
• Synonyms: AI841987; D330025K09; dgkd-2; DGKdelta
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215309 representing NM_177646
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGGCGCCCCTCCGCCGGGTCCTCCTCAGCCACCTCCGCCGCCGCCGCCTGAGGAGT
  CGTCCGACAGTGAACCCGAGGCTGAGCCGGGCTCCCCGCAGAAGCTTATCCGTAAGGTGTCCACGTCGGG
  CCAGATCCGGCAGAAGACCATCCTCAAAGAAGGCATGCTGACCAAACAGAACAACTCGTTCCAGCGATCC
  AAGAGGAGGTACTTTAAGCTTCGAGGGCGAACACTTTACTATGCTAAAACTGCAAAGTCGATTATATTTG
  ATGAGGTAGACCTGACTGATGCCAGCGTAGCAGAATCCAGCACCAAAAATGTCAACAACAGTTTTACGGT
  CATCACTCCATGTAGGAAGCTCATCTTGTGTGCCGACAACAGGAAAGAAATGGAAGATTGGATTGCAGCA
  CTCAAGACTGTCCAGAACAAAGAGCATTTTGAGCCTACCCAATATAGCATGGACCATTTCTCAGGGATGC
  ACAACTGGTATGCTTGTTCCCATGCACGGCCAACCTATTGCAATGTGTGTCGTGAAGTTTTGTCAGGGGT
  CACATCCCATGGGCTATCCTGTGAAGTGTGTAAGTTTAAGGCTCACAAACGTTGTGCTGTGCGTGCAACT
  AGTAACTGCAAGTGGACCACATTGGCCTCAATCGGGAAGGATATCATTGAGGACGAGGATGGGATTGCCA
  TGCCTCACCAGTGGTTGGAAGGAAACCTGCCTGTGAGTGCCAAGTGTATCGTGTGTGACAAGACTTGTGG
  CAGCGTGTTACGCCTGCAGGACTGGCGCTGCCTCTGGTGCAAAGCCATGGTTCACACATCTTGCAAAGAG
  TCCTTGGTGATGAAGTGTCCACTTGGCCTGTGCAAGGTGTCTGTCATCCCTCCCACGGCACTCAACAGTA
  TTGACTCCGATGGGTTCTGGAAGGCCACCTGTCCCCCGTCTTGTACAAGCCCTCTGCTGGTTTTTGTCAA
  CTCAAAAAGTGGAGACAATCAAGGTGTGAAGTTTCTCAGAAGATTCAAGCAGCTGCTGAACCCTGCCCAG
  GTTTTTGACCTCATGAATGGAGGACCACACCTTGGCTTACGGTTATTTCAGAAGTTTGACACCTTCCGGA
  TTCTGGTTTGTGGCGGGGATGGCAGTGTTGGCTGGGTCCTCTCTGAAATCGATAGCCTCAACCTTCACAA
  ACAGTGTCAGCTAGGTGTGCTGCCCCTGGGGACAGGGAACGACCTGGCCCGTGTTCTTGGCTGGGGCTCT
  GCCTGTGATGATGATACACAGCTTCCTCAGATCTTGGCAAAGCTGGAGAGGGCCAGCACTAAGATGCTGG
  ACAGGTGGAGTGTCATGGCATATGAGACCAAGCTCCCTCGGCAGGCCTCCTCCTCCACTGTCACTGAGGA
  CTTCAGCGAGGACTCAGAGGTGCAGCAAATTCTCTTCTATGAAGACTCAGTCGCAGCTCATCTTTCTAAA
  ATCTTAACTTCGGACCAGCATTCTGTGGTGATCTCATCAGCCAAAGTGCTCTGTGAGACAGTGAAGGACT
  TTGTGGCTCGAGTGGGCAAGGCCTATGAGAAGACGACAGAGAGCTCGCAGGAGTCAGAGGTCATGGCCAA
  GAAGTGCTCTGTCTTGAAAGAGAAACTGGACTCTCTCCTCAAGACCTTGGATGACGAGTCTCAGGCTTCA
  TCCTCTCTGTCTAACCCACCTCCTACCATTGCTGAGGAGGCTGAGGATGGGGACGGATCAGGCAACATCT
  GTAGCTCTACTGGGGACCATTTGGTGGGCTCGGCATGCCCCTCCCGGCCACAGATCTTCCGGCCCCGGGA
  GCAGCTAATGCTGAGAGCCAACAGCCTGAAGAAAGCAATTCGCCAGATTATAGAGCACACAGAAAAAGCG
  GTGGATGAGCAGAATGCCCAGACCCAGGAGCAGCAGGGTTTTGTCCTAGGCCTCTCTGAATCTGAGAAGA
  AGGACTTGAAGACTGACAACAGGGTGTGCACATCATCTGTCCATAGTGAGAGCTGTGTGATTGCCAAGGG
  GAGAAGTCAACGCAAAGCATCCAGGGCCCCTTGTGAGAAGCTGGTGAGCAAAGGCCTGTCACTCGGCAGC
  TCTGCCTCACTCCCTCCTGGTACAGGAAGCCGGGACAGCCTGCCTGCTCTGAACACCAAGATCCTATATC
  CAAGTGTTCGGGCAGGGATGTCTGGCTCCTTGCCAGGGGGCTCAGTCATCAGTCGCTTGTTAATTAATGC
  TGATCCCTTTAATGCTGAACCTGAAAACCTAGAATACTACACAGAGAAGTGTGTCATGAACAACTATTTT
  GGCATTGGCTTGGATGCAAAGATATCTCTGGACTTCAACAACAAGCGTGATGAGCACCCAGAGAAATGCA
  GGAGTAGGACTAAGAACATGATGTGGTATGGTGTCCTTGGCACCAAAGAGCTGCTGCACAGAACCTACAG
  GAACCTGGAACAAAAGGTCCTGCTGGAGTGTGATGGCCGCCCTATTCCCCTCCCGAGTCTTCAAGGAATT
  GCTGTCCTCAACATTCCCAGCTATGCTGGAGGGACCAACTTCTGGGGGGGCACCAAGGAAGATGATACTT
  TTGCAGCTCCATCATTCGATGATAAGATTCTGGAGGTGGTTGCTGTGTTCGGCAGCATGCAGATGGCTGT
  GTCTCGTGTAATTAAGCTACAGCATCATCGGATTGCCCAGTGTCGCACAGTGAAGATCTCCATCCTGGGG
  GATGAAGGCGTGCCTGTGCAGGTGGATGGGGAAGCCTGGATCCAGCCACCTGGGTACATTCGGATCGTGC
  ACAAGAACAGGGCGCAGACATTGACCAGAGACAGGGCCTTTGAAAACACCCTGAAGTCTTGGGAAGACAA
  ACAGAAGTGTGAGCTGTCCCGCCCACCCTCCTTTTCCCTGCACCCTGAGATCCTGTCTGAGGAGGAAGCT
  ACCCAGATGGATCAGTTTGGGCAGGCAGCAGGGGGCCTCATTCACAGCATTCGGGAGATAGCACAGTCCC
  ACAGAGCCATGGAACAGGAGCTCGCACATGCTGTCAATGCCAGCTCCAAAGCCATGGAGCGAGTATACGG
  CAAGCCCAGAACTGCAGAGGGGTTGAACTGTAGCTTTGTTCTGGAGATGGTGAATAACATCAGAGCACTG
  CGCAGTGAGACAGAGCTGCTGCTGGCTGGGAAGATGGCCCTGCAATTGGATCCCCCTCAGAAGGAACGGC
  TCGGGGCTGCCCTAATTGAGATGGACCAGCAGCTCAGGAAGCTGACAGACACTCCCTGGCTTTGCCAGCC
  CTTGGAGCCTGGTGAGGAAGAGTCTCTCCAACAGAATGTGATGCTGGATCTTACTAAACGCAGCCGTAGT
  GGTAAATTCCGCCTTGTGACCAAGTTTAAAAAGGAGAAAAACAATAAGAACAAAGAAGTTCACAGTAACC
  TAGGAGGCCCTGTTCACCTCTGGGGGACAGAGGAGGTTGCTGCCTGGCTGGAGCACCTCAGTCTCTGTGA
  GTATAAGGACATCTTCACGCGGCACGACATCCGGGGCTCTGAGCTCCTGCACCTGGAGCGGAGGGACCTC
  AAGGACCTTGGTGTGACCAAAGTGGGCCATATGAAGAGGATCCTTTGTGGGATCAAGGAGCTGAGCCGGA
  GCTCCCCTGCCGCCGAGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215309 representing NM_177646
  Red=Cloning site Green=Tags(s)
  
  MAAAAGAPPPGPPQPPPPPPPEESSDSEPEAEPGSPQKLIRKVSTSGQIRQKTILKEGMLTKQNNSFQRS
  KRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAA
  LKTVQNKEHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREVLSGVTSHGLSCEVCKFKAHKRCAVRAT
  SNCKWTTLASIGKDIIEDEDGIAMPHQWLEGNLPVSAKCIVCDKTCGSVLRLQDWRCLWCKAMVHTSCKE
  SLVMKCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQ
  VFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGS
  ACDDDTQLPQILAKLERASTKMLDRWSVMAYETKLPRQASSSTVTEDFSEDSEVQQILFYEDSVAAHLSK
  ILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTTESSQESEVMAKKCSVLKEKLDSLLKTLDDESQAS
  SSLSNPPPTIAEEAEDGDGSGNICSSTGDHLVGSACPSRPQIFRPREQLMLRANSLKKAIRQIIEHTEKA
  VDEQNAQTQEQQGFVLGLSESEKKDLKTDNRVCTSSVHSESCVIAKGRSQRKASRAPCEKLVSKGLSLGS
  SASLPPGTGSRDSLPALNTKILYPSVRAGMSGSLPGGSVISRLLINADPFNAEPENLEYYTEKCVMNNYF
  GIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYRNLEQKVLLECDGRPIPLPSLQGI
  AVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILG
  DEGVPVQVDGEAWIQPPGYIRIVHKNRAQTLTRDRAFENTLKSWEDKQKCELSRPPSFSLHPEILSEEEA
  TQMDQFGQAAGGLIHSIREIAQSHRAMEQELAHAVNASSKAMERVYGKPRTAEGLNCSFVLEMVNNIRAL
  RSETELLLAGKMALQLDPPQKERLGAALIEMDQQLRKLTDTPWLCQPLEPGEEESLQQNVMLDLTKRSRS
  GKFRLVTKFKKEKNNKNKEVHSNLGGPVHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDL
  KDLGVTKVGHMKRILCGIKELSRSSPAAEA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177646
• ORF Size: 3660 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177646.3, NP_808314.2
• RefSeq Size: 5694 bp
• RefSeq ORF: 3663 bp
• Locus ID: 227333
• UniProt ID: E9PUQ8
• Cytogenetics: 1 D
• Gene Summary: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:17021016). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:17021016). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development (PubMed:17021016). May also regulate clathrin-dependent endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]