Dgkd (NM_177646) Mouse Untagged Clone

CAT#: MC224002

Dgkd (untagged) - Mouse diacylglycerol kinase, delta (Dgkd), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dgkd
• Synonyms: AI841987; D330025K09; dgkd-2; DGKdelta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC224002 representing NM_177646
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCGGCGGGCGCCCCTCCGCCGGGTCCTCCTCAGCCACCTCCGCCGCCGCCGCCTGAGGAGT
  CGTCCGACAGTGAACCCGAGGCTGAGCCGGGCTCCCCGCAGAAGCTTATCCGTAAGGTGTCCACGTCGGG
  CCAGATCCGGCAGAAGACCATCCTCAAAGAAGGCATGCTGACCAAACAGAACAACTCGTTCCAGCGATCC
  AAGAGGAGGTACTTTAAGCTTCGAGGGCGAACACTTTACTATGCTAAAACTGCAAAGTCGATTATATTTG
  ATGAGGTAGACCTGACTGATGCCAGCGTAGCAGAATCCAGCACCAAAAATGTCAACAACAGTTTTACGGT
  CATCACTCCATGTAGGAAGCTCATCTTGTGTGCCGACAACAGGAAAGAAATGGAAGATTGGATTGCAGCA
  CTCAAGACTGTCCAGAACAAAGAGCATTTTGAGCCTACCCAATATAGCATGGACCATTTCTCAGGGATGC
  ACAACTGGTATGCTTGTTCCCATGCACGGCCAACCTATTGCAATGTGTGTCGTGAAGTTTTGTCAGGGGT
  CACATCCCATGGGCTATCCTGTGAAGTGTGTAAGTTTAAGGCTCACAAACGTTGTGCTGTGCGTGCAACT
  AGTAACTGCAAGTGGACCACATTGGCCTCAATCGGGAAGGATATCATTGAGGACGAGGATGGGATTGCCA
  TGCCTCACCAGTGGTTGGAAGGAAACCTGCCTGTGAGTGCCAAGTGTATCGTGTGTGACAAGACTTGTGG
  CAGCGTGTTACGCCTGCAGGACTGGCGCTGCCTCTGGTGCAAAGCCATGGTTCACACATCTTGCAAAGAG
  TCCTTGGTGATGAAGTGTCCACTTGGCCTGTGCAAGGTGTCTGTCATCCCTCCCACGGCACTCAACAGTA
  TTGACTCCGATGGGTTCTGGAAGGCCACCTGTCCCCCGTCTTGTACAAGCCCTCTGCTGGTTTTTGTCAA
  CTCAAAAAGTGGAGACAATCAAGGTGTGAAGTTTCTCAGAAGATTCAAGCAGCTGCTGAACCCTGCCCAG
  GTTTTTGACCTCATGAATGGAGGACCACACCTTGGCTTACGGTTATTTCAGAAGTTTGACACCTTCCGGA
  TTCTGGTTTGTGGCGGGGATGGCAGTGTTGGCTGGGTCCTCTCTGAAATCGATAGCCTCAACCTTCACAA
  ACAGTGTCAGCTAGGTGTGCTGCCCCTGGGGACAGGGAACGACCTGGCCCGTGTTCTTGGCTGGGGCTCT
  GCCTGTGATGATGATACACAGCTTCCTCAGATCTTGGCAAAGCTGGAGAGGGCCAGCACTAAGATGCTGG
  ACAGGTGGAGTGTCATGGCATATGAGACCAAGCTCCCTCGGCAGGCCTCCTCCTCCACTGTCACTGAGGA
  CTTCAGCGAGGACTCAGAGGTGCAGCAAATTCTCTTCTATGAAGACTCAGTCGCAGCTCATCTTTCTAAA
  ATCTTAACTTCGGACCAGCATTCTGTGGTGATCTCATCAGCCAAAGTGCTCTGTGAGACAGTGAAGGACT
  TTGTGGCTCGAGTGGGCAAGGCCTATGAGAAGACGACAGAGAGCTCGCAGGAGTCAGAGGTCATGGCCAA
  GAAGTGCTCTGTCTTGAAAGAGAAACTGGACTCTCTCCTCAAGACCTTGGATGACGAGTCTCAGGCTTCA
  TCCTCTCTGTCTAACCCACCTCCTACCATTGCTGAGGAGGCTGAGGATGGGGACGGATCAGGCAACATCT
  GTAGCTCTACTGGGGACCATTTGGTGGGCTCGGCATGCCCCTCCCGGCCACAGATCTTCCGGCCCCGGGA
  GCAGCTAATGCTGAGAGCCAACAGCCTGAAGAAAGCAATTCGCCAGATTATAGAGCACACAGAAAAAGCG
  GTGGATGAGCAGAATGCCCAGACCCAGGAGCAGCAGGGTTTTGTCCTAGGCCTCTCTGAATCTGAGAAGA
  AGGACTTGAAGACTGACAACAGGGTGTGCACATCATCTGTCCATAGTGAGAGCTGTGTGATTGCCAAGGG
  GAGAAGTCAACGCAAAGCATCCAGGGCCCCTTGTGAGAAGCTGGTGAGCAAAGGCCTGTCACTCGGCAGC
  TCTGCCTCACTCCCTCCTGGTACAGGAAGCCGGGACAGCCTGCCTGCTCTGAACACCAAGATCCTATATC
  CAAGTGTTCGGGCAGGGATGTCTGGCTCCTTGCCAGGGGGCTCAGTCATCAGTCGCTTGTTAATTAATGC
  TGATCCCTTTAATGCTGAACCTGAAAACCTAGAATACTACACAGAGAAGTGTGTCATGAACAACTATTTT
  GGCATTGGCTTGGATGCAAAGATATCTCTGGACTTCAACAACAAGCGTGATGAGCACCCAGAGAAATGCA
  GGAGTAGGACTAAGAACATGATGTGGTATGGTGTCCTTGGCACCAAAGAGCTGCTGCACAGAACCTACAG
  GAACCTGGAACAAAAGGTCCTGCTGGAGTGTGATGGCCGCCCTATTCCCCTCCCGAGTCTTCAAGGAATT
  GCTGTCCTCAACATTCCCAGCTATGCTGGAGGGACCAACTTCTGGGGGGGCACCAAGGAAGATGATACTT
  TTGCAGCTCCATCATTCGATGATAAGATTCTGGAGGTGGTTGCTGTGTTCGGCAGCATGCAGATGGCTGT
  GTCTCGTGTAATTAAGCTACAGCATCATCGGATTGCCCAGTGTCGCACAGTGAAGATCTCCATCCTGGGG
  GATGAAGGCGTGCCTGTGCAGGTGGATGGGGAAGCCTGGATCCAGCCACCTGGGTACATTCGGATCGTGC
  ACAAGAACAGGGCGCAGACATTGACCAGAGACAGGGCCTTTGAAAACACCCTGAAGTCTTGGGAAGACAA
  ACAGAAGTGTGAGCTGTCCCGCCCACCCTCCTTTTCCCTGCACCCTGAGATCCTGTCTGAGGAGGAAGCT
  ACCCAGATGGATCAGTTTGGGCAGGCAGCAGGGGGCCTCATTCACAGCATTCGGGAGATAGCACAGTCCC
  ACAGAGCCATGGAACAGGAGCTCGCACATGCTGTCAATGCCAGCTCCAAAGCCATGGAGCGAGTATACGG
  CAAGCCCAGAACTGCAGAGGGGTTGAACTGTAGCTTTGTTCTGGAGATGGTGAATAACATCAGAGCACTG
  CGCAGTGAGACAGAGCTGCTGCTGGCTGGGAAGATGGCCCTGCAATTGGATCCCCCTCAGAAGGAACGGC
  TCGGGGCTGCCCTAATTGAGATGGACCAGCAGCTCAGGAAGCTGACAGACACTCCCTGGCTTTGCCAGCC
  CTTGGAGCCTGGTGAGGAAGAGTCTCTCCAACAGAATGTGATGCTGGATCTTACTAAACGCAGCCGTAGT
  GGTAAATTCCGCCTTGTGACCAAGTTTAAAAAGGAGAAAAACAATAAGAACAAAGAAGTTCACAGTAACC
  TAGGAGGCCCTGTTCACCTCTGGGGGACAGAGGAGGTTGCTGCCTGGCTGGAGCACCTCAGTCTCTGTGA
  GTATAAGGACATCTTCACGCGGCACGACATCCGGGGCTCTGAGCTCCTGCACCTGGAGCGGAGGGACCTC
  AAGGACCTTGGTGTGACCAAAGTGGGCCATATGAAGAGGATCCTTTGTGGGATCAAGGAGCTGAGCCGGA
  GCTCCCCTGCCGCCGAGGCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177646
• Insert Size: 3663 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177646.3, NP_808314.2
• RefSeq Size: 5694 bp
• RefSeq ORF: 3663 bp
• Locus ID: 227333
• UniProt ID: E9PUQ8
• Cytogenetics: 1 D
• Gene Summary: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:17021016). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:17021016). By controlling the levels of diacylglycerol, regulates for instance the PKC and EGF receptor signaling pathways and plays a crucial role during development (PubMed:17021016). May also regulate clathrin-dependent endocytosis (By similarity).[UniProtKB/Swiss-Prot Function]