Selenon (NM_029100) Mouse Tagged ORF Clone

CAT#: MG215232

Sepn1 (GFP-tagged) - Mouse selenoprotein N 1 (Sepn1), (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below)

Product Images

Circular map for MG215232

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Symbol: Selenon
• Synonyms: 1110019I12Rik; AI414492; Se; SelN; Sepn1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215232 representing NM_029100
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCAGGCCAGGCCCGCAGCGCGCCGCCCGCACAGCCCGGACCCCGGCGCTCAGCCCGCGCCTCCAC
  GCCGCCGCGCCCGCGCCCTGGCGCTGCTCGGAGCCCTGCTGGCGGCCGCCGCCGCTGTCGCCGCTGCCCG
  GGCCTGCGCGCTCCTCGCCGACGCCCAGGCGGCCGCGCGGCAGGAATCGGCATTAAAGGTCCTGGGGACA
  GATGGCCTCTTTCTCTTTTCCTCGCTGGACACTGACCAGGATATGTACATCAGTCCGGAGGAATTCAAAC
  CCATTGCGGAGAAACTGACAGGGTCAGTTCCTGTGGCCAACTACGAGGAGGAGGAACTGCCCCATGACCC
  CAGCGAGGAGACTCTTACCATAGAAGCCCGATTCCAGCCTCTGCTCATGGAGACCATGACCAAAAGCAAA
  GATGGCTTCCTAGGGGTCTCCCGCCTCGCTCTGTCCGGCCTCCGCAACTGGACCACAGCAGCCTCGCCGA
  GTGCAGCGTTTGCTGCCCGCCACTTCCGGCCCTTCCTGCCCCCTCCAGGTCAGGAGCTGGGCCAGCCCTG
  GTGGATTATCCCCGGGGAGCTGAGCGTCTTCACGGGCTATTTGTCCAACAACCGCTTCTACCCACCACCG
  CCCAAGGGCAAGGAGGTCATCATCCATCGCCTGTTAAGCATGTTCCATCCACGCCCCTTCGTGAAGACCC
  GCTTTGCCCCTCAGGGCACCGTGGCCTGTCTGACTGCCATCAGCGATTCCTACTACACCGTGATGTTCCG
  GATCCACGCGGAGTTTCAGCTCAGCGAGCCTCCTGACTTCCCCTTCTGGTTCTCGCCCGGCCAGTTCACC
  GGCCATATCATCTTATCCAAAGATGCTACACACATCCGTGACTTCAGGCTGTTTGTGCCCAATCACAGGT
  CCCTGAATGTGGACATGGAGTGGCTGTATGGGGCCAGTGAGACCAGCAACATGGAGGTGGACATTGGCTA
  CGTCCCCCAGATGGAGCTGGAGGCTGTGGGCCCCTCGGTGCCCTCTGTGATCCTAGATGAGGACGGCAAC
  ATGATTGACAGCCGCCTGCCCTCAGGAGAACCCCTCCAGTTTGTGTTTGAGGAGATCAAGTGGCATCAGG
  AGCTGAGCTGGGAAGAGGCTGCCCGGCGCCTGGAGGTGGCCATGTATCCCTTCAAGAAGGTCAACTACCT
  GCCGTTCACGGAGGCCTTTGACAGAGCCAGAGCTGAGAAGAAACTTGTTCATTCCATCTTGCTGTGGGGG
  GCCCTGGACGACCAGTCCTGCTGAGGTTCCGGGCGGACTCTCCGGGAGACTGTCCTGGAAAGCCCGCCCA
  TCCTCACTCTCCTCAATGAGAGCTTCATCAGTACCTGGTCCCTGGTAAAGGAGCTAGAAGACCTGCAGAC
  CCAGCAGGAGAACCCACTCCACAGGCAGCTGGCAGGCCTGCACTTGGAGAAGTACAGCTTTCCTGTAGAG
  ATGATGATCTGTCTGCCCAACGGCACTGTGGTCCACCACATCAATGCCAACTACTTCCTTGACATCACCT
  CCATGAAGCCTGAAGACATGGAGAATAATAACGTCTTCAGCTTCTCATCCAGCTTTGAGGACCCGTCCAC
  AGCTACCTACATGCAGTTCCTGAGGGAAGGACTCCGGCGGGGCCTGCCCCTCCTCCAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215232 representing NM_029100
  Red=Cloning site Green=Tags(s)
  
  MGQARPAARRPHSPDPGAQPAPPRRRARALALLGALLAAAAAVAAARACALLADAQAAARQESALKVLGT
  DGLFLFSSLDTDQDMYISPEEFKPIAEKLTGSVPVANYEEEELPHDPSEETLTIEARFQPLLMETMTKSK
  DGFLGVSRLALSGLRNWTTAASPSAAFAARHFRPFLPPPGQELGQPWWIIPGELSVFTGYLSNNRFYPPP
  PKGKEVIIHRLLSMFHPRPFVKTRFAPQGTVACLTAISDSYYTVMFRIHAEFQLSEPPDFPFWFSPGQFT
  GHIILSKDATHIRDFRLFVPNHRSLNVDMEWLYGASETSNMEVDIGYVPQMELEAVGPSVPSVILDEDGN
  MIDSRLPSGEPLQFVFEEIKWHQELSWEEAARRLEVAMYPFKKVNYLPFTEAFDRARAEKKLVHSILLWG
  ALDDQSC*GSGRTLRETVLESPPILTLLNESFISTWSLVKELEDLQTQQENPLHRQLAGLHLEKYSFPVE
  MMICLPNGTVVHHINANYFLDITSMKPEDMENNNVFSFSSSFEDPSTATYMQFLREGLRRGLPLLQP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029100
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info The expression of this clone is not guaranteed due to the nature of selenoproteins.
• OTI Annotation: This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029100.2, NP_083376.2
• RefSeq Size: 3461 bp
• RefSeq ORF: 1674 bp
• Locus ID: 74777
• UniProt ID: D3Z2R5
• Cytogenetics: 4 D3
• Gene Summary: This gene encodes a glycoprotein that is localized in the endoplasmic reticulum. It plays an important role in cell protection against oxidative stress, and in the regulation of redox-related calcium homeostasis. Mutations in the orthologous gene in human are associated with early onset muscle disorders, referred to as SEPN1-related myopathy. Knockout mice deleted for this gene exhibit abnormal lung development. This protein is a selenoprotein, containing the rare amino acid selenocysteine (Sec). Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A second stop-codon redefinition element (SRE) adjacent to the UGA codon has been identified in this gene (PMID:15791204). SRE is a phylogenetically conserved stem-loop structure that stimulates readthrough at the UGA codon, and augments the Sec insertion efficiency by SECIS. [provided by RefSeq, Dec 2016]