Adh6a (NM_026945) Mouse Tagged ORF Clone

CAT#: MG215192

Adh6a (tGFP-tagged) - Mouse alcohol dehydrogenase 6A (class V) (Adh6a), (10ug)

Product Images

Circular map for MG215192

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Adh6a
• Synonyms: 1810009C16Rik; Adh5a
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215192 representing NM_026945
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATACCCTGGGAAAGACAATCACATGTCGGGCAGCCATTGCCTGGGCAAAAAATTCTCCTCTTTCAA
  TTGAAGAAGTGCAAGTTGAGCCACCGAAGTCTGGAGAAGTTCGTATTAAGATGATCTCGTCAGGGATCTG
  TGGTTCTGATGATCACGTGCTGAAAGGAGAACTCGTGGTGAACTTTCCTTTAATTCCGGGTCATGAAGGA
  GCGGGAATTGTGGAGAGTGTTGGTGATGGAGTTTGCTCCATGAAACCAGGAGATAAAGTCCTCATCCTCA
  TTATTCCACAGTGTAGAGAATGTGACGCCTGCTTGTACCCCAAAGGAAATTTCTGCTACAAGCAAGATGT
  TCTGCCTTGTTCTGGGGTGATGCTCGACGGGACCTCCCGGTTTTCCTGCCGAGGACAGAAGATTTATCAC
  TCTTTCCGAACAAGCTCATTCACAGAGTACACTGTGGTGCCCGAGATCGCCGCGGTAAAAATTGATGACG
  CAGCTCCTATGGATAAAGTTTGTCTCATAAGCTGTGGCTTCCCTACTGGCTACGGGGCTGCTGTTAACTC
  TGCCAAGGTCACCCCTGGCTCTACCTGTGTGGTTTTTGGACTCGGAGGGGTCGGCTCAGCTATTGTCATG
  GGCTGCAAAGCCTCTGGTGCATCTAGAATCATCGGGGTTGACATCAATGAGGAGAAGTTTCCTCGGGCAA
  GAGCCTTGGGGGTCACCGACTGTCTCAACCCTAACAAGCTGGAGAAGCCTGTGCAGGAGGTTGTGATGGA
  AATGACGGGTGTCGGTGTTGACTTTGCCTTTGAGGCCATTGGACTGGTTGATACCATGGTTGCTGCTTGG
  AACTCCTGCAACAACAGCTATGGCGTCTGTCTGATCGCTGGGCTCGCTCCATCAGATGCACAGTTATCCC
  TTGAAGCACCCAAGATTTTGTCTGGGAAAACCCTGAAGGGCGTGTGTCTGGGAGATTATAAAACCAGAGA
  CTGCATACCCCAAATAGTGACTGATTATCTCCAAAATAAGATTAATATAGATCCTTTAGTGACTTATCAG
  CTGCCTTTTAACCAGCTCCATGAAGCCTTGGAGCTGTTTCATTCAGGAAAAGCTATCCGTTGTGTTCTGC
  TCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215192 representing NM_026945
  Red=Cloning site Green=Tags(s)
  
  MNTLGKTITCRAAIAWAKNSPLSIEEVQVEPPKSGEVRIKMISSGICGSDDHVLKGELVVNFPLIPGHEG
  AGIVESVGDGVCSMKPGDKVLILIIPQCRECDACLYPKGNFCYKQDVLPCSGVMLDGTSRFSCRGQKIYH
  SFRTSSFTEYTVVPEIAAVKIDDAAPMDKVCLISCGFPTGYGAAVNSAKVTPGSTCVVFGLGGVGSAIVM
  GCKASGASRIIGVDINEEKFPRARALGVTDCLNPNKLEKPVQEVVMEMTGVGVDFAFEAIGLVDTMVAAW
  NSCNNSYGVCLIAGLAPSDAQLSLEAPKILSGKTLKGVCLGDYKTRDCIPQIVTDYLQNKINIDPLVTYQ
  LPFNQLHEALELFHSGKAIRCVLLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_026945
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_026945.1, NP_081221.1
• RefSeq Size: 1341 bp
• RefSeq ORF: 1128 bp
• Locus ID: 69117
• Cytogenetics: 3 G3