Tmem259 (NM_001003949) Mouse Tagged ORF Clone

CAT#: MG215186

Tmem259 (tGFP-tagged) - Mouse open reading frame 61 (ORF61), (10ug)

Product Images

Circular map for MG215186

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmem259
• Synonyms: AC087114.7gm2; mFLJ00277; ORF61; R32184_3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG215186 representing NM_001003949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGCACGCGGCAGCCCCAGGGCCCGGGCCCAACGGCGGCGGGGGTGGTGGCGCGGCGCCCGTGC
  GCGGCCCTCGCGGCCCCAATCTCAACCCCAACCCGCTCATCAACGTGCGCGACCGGCTCTTCCACGCTCT
  CTTCTTCAAGATGGCCGTCACCTACTCACGCCTCTTCCCACCTGCTTTCCGCCGCCTCTTTGAATTCTTC
  GTTCTGCTCAAGGCCCTGTTTGTGCTTTTCGTCCTCGCCTACATACACATCGTCTTCTCCCGGTCCCCCA
  TCAACTGCTTAGAGCATGTTCGAGATCGATGGCCGCGGGAGGGTGTCCTGCGGGTGGAGGTGCGCCACAA
  CTCGAGCCGGGCACCGGTGATCCTGCAGTTCTGTGATGGGGGCCTCGGTGGCCTGGAGCTGGAACCCGGG
  GGCCTGGAGCTGGAGGAGGAGGAGCTCACAGTGGAGATGTTCACCAACAGCTCCATCAAGTTTGAGCTGG
  ACATTGAGCCCAAGGTGTTCAAGCCACAGAGCGGTGCAGATGCCCTGAACGACAGCCAGGACTTCCCTTT
  TCCTGAGACGCCAGCAAAAGTGTGGCCACAGGATGAGTACATTGTGGAGTACTCACTGGAATATGGCTTC
  CTGCGGCTATCCCAAGCCACACGCCAGCGTCTGAGCATTCCTGTCATGGTGGTCACCCTAGACCCCACGC
  GGGACCAGTGCTTTGGGGACCGCTTCAGCCGCCTATTGCTGGATGAGTTCCTGGGCTATGATGACATCCT
  CATGTCCAGTGTAAAGGGTCTGGCAGAGAACGAGGAGAACAAAGGCTTCTTGAGGAATGTGGTCTCTGGG
  GAGCACTACCGCTTTGTCAGCATGTGGATGGCGCGCACATCCTACCTGGCGGCCTTTGTCATCATGGTCA
  TCTTTACCCTCAGCGTGTCCATGCTGTTGCGATACTCGCACCACCAGATCTTCGTCTTCATCGTGGACCT
  GCTGCAGATGCTGGAGATGAACATGGCCATCGCCTTCCCCGCAGCGCCCTTGCTGACCGTCATCCTGGCT
  CTCGTCGGGATGGAAGCCATCATGTCTGAGTTCTTCAATGATACCACCACGGCCTTCTACATCATCCTCA
  CTGTGTGGCTGGCCGACCAGTATGATGCCATCTGCTGCCACACCAACACCAGCAAGCGGCACTGGCTGAG
  GTTCTTCTACCTCTACCACTTCGCCTTCTATGCCTACCACTACCGCTTTAACGGGCAGTACAGCAGCCTG
  GCCCTGGTCACCTCCTGGCTCTTCATCCAGCATTCCATGATCTACTTCTTCCACCACTATGAGTTGCCCG
  CCATCCTGCAGCAGATCCGAATCCAGGAGATGCTGCTTCAGACGCCACCGCTGGGCCCCGGGACCCCCAC
  GGCGCTGCCTGACGACCTCAACAACAACTCTGGCTCCCCTGCCACTCCGGATCCCAGCCCTCCCCTCGCG
  CTGGGCCCCAGCTCCAGCCCCGCGCCCACTGGCGGGGCATCTGGGCCTGGCTCACTGGGCGCTGGGGCCT
  CAGTATCCGGCAGTGACCTAGGTTGGGTGGCCGAGACCGCCGCCATCATCTCTGACGCATCCTTCCTGTC
  GGGGCTGAGCGCCTCTCTCCTGGAGCGGCGGCCAACAGCCCCTAGTACCCCGGACAGCTCACGACCTGAC
  CCTGGGGTCCCTCTGGAGGACGCACCCGCCCCTGCCGGGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG215186 representing NM_001003949
  Red=Cloning site Green=Tags(s)
  
  MSEHAAAPGPGPNGGGGGGAAPVRGPRGPNLNPNPLINVRDRLFHALFFKMAVTYSRLFPPAFRRLFEFF
  VLLKALFVLFVLAYIHIVFSRSPINCLEHVRDRWPREGVLRVEVRHNSSRAPVILQFCDGGLGGLELEPG
  GLELEEEELTVEMFTNSSIKFELDIEPKVFKPQSGADALNDSQDFPFPETPAKVWPQDEYIVEYSLEYGF
  LRLSQATRQRLSIPVMVVTLDPTRDQCFGDRFSRLLLDEFLGYDDILMSSVKGLAENEENKGFLRNVVSG
  EHYRFVSMWMARTSYLAAFVIMVIFTLSVSMLLRYSHHQIFVFIVDLLQMLEMNMAIAFPAAPLLTVILA
  LVGMEAIMSEFFNDTTTAFYIILTVWLADQYDAICCHTNTSKRHWLRFFYLYHFAFYAYHYRFNGQYSSL
  ALVTSWLFIQHSMIYFFHHYELPAILQQIRIQEMLLQTPPLGPGTPTALPDDLNNNSGSPATPDPSPPLA
  LGPSSSPAPTGGASGPGSLGAGASVSGSDLGWVAETAAIISDASFLSGLSASLLERRPTAPSTPDSSRPD
  PGVPLEDAPAPAGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001003949
• ORF Size: 1722 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001003949.4
• RefSeq Size: 2223 bp
• RefSeq ORF: 1725 bp
• Locus ID: 216157
• UniProt ID: Q8CIV2
• Cytogenetics: 10 C1
• Gene Summary: May have a role in the ERAD pathway required for clearance of misfolded proteins in the endoplasmic reticulum (ER). Promotes survival of motor neurons, probably by protecting against ER stress.[UniProtKB/Swiss-Prot Function]