Pabpc6 (NM_001163836) Mouse Tagged ORF Clone

CAT#: MG214930

Pabpc6 (tGFP-tagged) - Mouse poly(A) binding protein cytoplasmic 6 (Pabpc6), (10ug)

Product Images

Circular map for MG214930

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pabpc6
• Synonyms: 4932702K14Rik; AI428050; Pabpc3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214930 representing NM_001163836
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCCAGCGACCCCAGCTACTCTTTGGCTTCACTCTATGTGGGGGACCTGCATCCCGACGTGACCG
  AGGCGATGCTCTATGAGAAGTTCAGCCCGGCAGGGCCCATCCTCTCCATCCGGGTGTACAGGGACAGGAT
  CACCCGCCGCTCGCTGGGCTACGCGTCCGTCAACTTCCAGCAGCTGGAGGACGCCGAGCGGGCCTTGGAC
  ACCATGAATTTCGACGTGATAAAGGGCAAGCCGGTTCGCATCATGTGGTCTCAGCGGGACCCGTCGCTGC
  GCAAGAGCGGAGTAGGGAACATATTTGTGAAAAATCTGGACAGATCCATTGACAGTAAAACGCTGTATGA
  TACGTTTTCTGCCTTTGGTAACATCCTTTCCTGTAAGGTGGTGTGTGATGAGAATGGCTCCAAGGGTTAC
  GGCTTTGTACACTTCGAGACACAGGAAGAAGCTGAACGAGCAATTGAGAAAATGAACGGGATGTTCCTAA
  ATGACCACAAAGTGTTCGTTGGTCGCTTTAAGTCTCGTAGAGACAGGCAGGCTGAACTTGGAGCCAGGGC
  CAAGGAGTTCACCAATGTTTACATCAAGAATTTGGGAGAAGATATGGATGATGAGAGACTCCAGGATCTA
  TTTGGCAGATTTGGGCCTGCCTTAAGTGTTAAAGTCATGACTGATGAAAGTGGAAAATCCAAAGGATTCG
  GATTCGTAAGCTTTGAAAGGCATGAAGATGCACGAAAGGCAGTGGAAGAGATGAATGGAAAAGACCTCAA
  TGGAAAACAAATTTATGTAGGTCGTGCTCAGAAAAAGGTGGAACGACAGACTGAACTGAAGCACAAATTC
  GGGCAGATGAAGCAAGATAAGCACAAAATTGAACGGGTACCACAAGATAGAAGCGTCAGATGCAAAGGTG
  TTAACCTCTATGTGAAAAACCTTGATGATGGTATCGATGATGAGCGTCTCCGGAAGGAGTTTTCCCCTTT
  TGGTACGATTACTAGTGCAAAGGTAACGATGGAGGGTGGGCGCAGCAAAGGGTTTGGTTTTGTATGTTTT
  TCCTCTCCAGAAGAAGCCACCAAAGCTGTTACAGAAATGAATGGTAAAATAGTGGCCACCAAACCACTCT
  ACGTAGCTTTAGCTCAACGCAAAGAGGAGCGCCAGGCTCACCTCAGTAACCAGTACATGCAAAGAATGGC
  AAGCACCAGTGCCGGGCCTAACCCAGTCGTCAGCCCTTTCCAGCCAGCGCAGGGGCCGTCAGGTTACTGC
  ATGACAGCTACACCAACTCAGAGCCGTAGGGCATACTGTGCTCCTAATCAGACTACTCAGCAAGGACCAA
  GTGCCCGTGGGAGTGCTCACGGTACCAGAGCTCATCCATTCCAAAACATGTCTAGTACCATCCACCCATC
  ACATACTATGCCATCATTTAGTACTTCAGGACCCACTACCTCACAGGCTATACGACATCGTACTGCCAGC
  ACATCCACACAGATGATGGGCCCACATCCTGCAGCTGCTGCTGCTGCTGCAGCTGCTCCTGCCACCCGCA
  CAATTACCCAGTATAAATATACTGCTGGAGTCCGAAATCCCCCTCAGCATCCTAATACACAGCCACATGT
  CAGCACGCAGCGGTCTGCTGTTCCTGTCCAAGGTAAAGAGTCTTTGACTGCCTCCATGTTGGCCTCTGCT
  CCTCCTCAAGCTCAAAAGCAAATGTTAGGGGAATGGCTCTTCTCTCTTATTCAGGCCATGCACCCTGCCC
  TTGCTGGTAAAATCACGGGTATGCTGTTGGAAATTGATAATATAGAGCTACGCCACATGCTTGAGTCTCC
  AGAGTGTCTCCACACCAAAGTTGATGAAGCCATAGCTGTACTGCAAGCTCACCAAGCTAAAGAGACTTCA
  CAGAAAGCAGTCAGCAGTGTTGCTGGTGTCCCAAATGCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214930 representing NM_001163836
  Red=Cloning site Green=Tags(s)
  
  MNPSDPSYSLASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVYRDRITRRSLGYASVNFQQLEDAERALD
  TMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFVKNLDRSIDSKTLYDTFSAFGNILSCKVVCDENGSKGY
  GFVHFETQEEAERAIEKMNGMFLNDHKVFVGRFKSRRDRQAELGARAKEFTNVYIKNLGEDMDDERLQDL
  FGRFGPALSVKVMTDESGKSKGFGFVSFERHEDARKAVEEMNGKDLNGKQIYVGRAQKKVERQTELKHKF
  GQMKQDKHKIERVPQDRSVRCKGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVTMEGGRSKGFGFVCF
  SSPEEATKAVTEMNGKIVATKPLYVALAQRKEERQAHLSNQYMQRMASTSAGPNPVVSPFQPAQGPSGYC
  MTATPTQSRRAYCAPNQTTQQGPSARGSAHGTRAHPFQNMSSTIHPSHTMPSFSTSGPTTSQAIRHRTAS
  TSTQMMGPHPAAAAAAAAAPATRTITQYKYTAGVRNPPQHPNTQPHVSTQRSAVPVQGKESLTASMLASA
  PPQAQKQMLGEWLFSLIQAMHPALAGKITGMLLEIDNIELRHMLESPECLHTKVDEAIAVLQAHQAKETS
  QKAVSSVAGVPNA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163836
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163836.1, NP_001157308.1
• RefSeq Size: 3208 bp
• RefSeq ORF: 1932 bp
• Locus ID: 67543
• Cytogenetics: 17 A1