Hbq1b (NM_001033981) Mouse Tagged ORF Clone

CAT#: MG214711

Hbq1b (tGFP-tagged) - Mouse RIKEN cDNA F830116E18 gene (F830116E18Rik), (10ug)

Product Images

Circular map for MG214711

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hbq1b
• Synonyms: F830116E18Rik; Hbqt1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214711 representing NM_001033981
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGTCCCAGGATGATCAGTGGCTGGTCCTTGCGCTCTGGAAGAAGATGGGCAGCAATGTCGGAA
  TCTACGCGACCGAGGCTTTGGAGAGGACCTTCGTGGCTTTCCCCTCCACCAAAACCTACTTCCCACACTT
  GGACCTGAGGCCAGGCTCTAGCCAGGTTAAAGCCCATGCCCAAAAGGTGGCCGATGCACTGACTCTCGCT
  ACCCAGCACCTGGACGACCTGCCTGCTTCTCTGTCTGCTCTCAGTGATCTACATGCGCACAAGCTCTGTG
  TGGACCCTGCTAACTTCCAGTTCTTCAGCCACTGTCTGCTGGTGACTCTCGCCAGGCACTATCCTGGAGA
  CTTCAGCCCAGAGATGCATGCCTCCTTGGACAAGTTTCTGGGTCACGTGACTTCGGCACTGGTCTCCAAA
  TATCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214711 representing NM_001033981
  Red=Cloning site Green=Tags(s)
  
  MARSQDDQWLVLALWKKMGSNVGIYATEALERTFVAFPSTKTYFPHLDLRPGSSQVKAHAQKVADALTLA
  TQHLDDLPASLSALSDLHAHKLCVDPANFQFFSHCLLVTLARHYPGDFSPEMHASLDKFLGHVTSALVSK
  YR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033981
• ORF Size: 426 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033981.3, NP_001029153.1
• RefSeq Size: 748 bp
• RefSeq ORF: 429 bp
• Locus ID: 544763
• Cytogenetics: 11 A4
• Gene Summary: This gene is one of two mouse theta-globin genes found in the alpha-globin gene cluster on chromosome 11. This gene represents the T1 (or 5') theta-globin gene described in PMIDs 18245844 and 11157800, respectively. [provided by RefSeq, Apr 2009]