Nol10 (NM_001008421) Mouse Tagged ORF Clone

CAT#: MG214070

Nol10 (tGFP-tagged) - Mouse nucleolar protein 10 (Nol10), (10ug)

Product Images

Circular map for MG214070

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nol10
• Synonyms: Gm67
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214070 representing NM_001008421
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGTCTCCAGCCTCAACGAAGTGAAGATTTACAGCCTCAGCTGCGGCAAGTCTCTTCCCGAGTGGC
  TTTCTGACAGGAAGAAGAGAGCACTGCAGAAGAAGAATGTCGATGTTCGTAGGAGAATTGAACTTATTCA
  GGACTTCGAAATGCCTACTGTGTGTACTACTATCAAGGTGTCAAAAGATGGACAGTATATTTTAGCAACT
  GGAACATATAAGCCTCGGGTCCGGTGTTATGACACATATCAGTTATCCCTGAAGTTTGAAAGGTGCTTGG
  ATTCTGAAGTTGTCACCTTTGAGATTTTATCAGATGACTATTCAAAGATTGTCTTCTTACATAATGACAG
  ATATATTGAATTTCACTCACAATCAGGCTTTTACTACAAAACCAGAATTCCCAAGTTCGGCCGAGACTTC
  TCTTACCACTATCCATCCTGTGACTTGTACTTTGTAGGTGCAAGCTCTGAAGTTTATAGATTAAACTTAG
  AACAAGGGCGGTACCTGAATCCTCTCCAGACGGACGCCGCGGAGAACAATGTTTGTGACATAAATGCAGT
  GCATGGCTTGTTCGCCACAGGAACAATAGAGGGTCGAGTGGAATGCTGGGATCCAAGAGTAAGAAAAAGG
  GTTGGCGTGTTAGACTGTGCATTAAATAGTGTCACAGCAGATTCAGAGATAAACAGCTTACCCACCATCT
  CTGCATTGAAGTTTAATGGAGCCTTGAGCATGGCAGTTGGAACATCCACAGGGCAGGTGTTGTTATATGA
  TCTTCGCTCGGATAAGCCGTTGCTAGTTAAGGATCACCAGTATGGGCTGCCGATTAAGTCAGTTCATTTC
  CAGGATTCCCTAGATTTGGTTTTGTCTGCAGACTCTAGAATTGTCAAAATGTGGAACAAGGACTCCGGGA
  AAATATTTACTTCGTTGGAGCCAGAACATGATCTTAATGATGTTTGCCTCTATCCCAGCTCAGGAATGCT
  TCTTACTGCCAATGAATCCCCCAAGATGGGCATTTACTACATTCCGGTTTTGGGTCCTGCTCCCAGGTGG
  TGTTCCTTCCTGGACAACTTGACAGAAGAGTTAGAAGAGAATCCAGAGAGCACAGTGTATGATGATTACA
  AATTTGTCACCAAGAAAGACCTTGAAAATTTAGGGCTCACACATCTCATTGGATCACCTTTCCTCCGAGC
  ATATATGCATGGGTTTTTCATGGATATCAGACTCTACCACAAGGTGAAATTGATGGTCAATCCATTTGCT
  TATGAAGAATATAGGAAAGATAAAATTCGACAGAAGATAGAAGAAACACGTGCACAGAGAGTCCAATTAA
  AGAAACTGCCAAAAGTTAACAAAGAACTGGCACTTAAATTAATTGAAGAGGAAGAGGAGAAACAAAAGTC
  TACGCTGAAAAAGAAAGTTAAGAGCCTCCCTAATATCCTCACGGATGATCGATTTAAAGTTATGTTTGAG
  AATCCCGACTTCCAGGTGGACGAGGAGAGTGAAGAGTTCAGGCTTCTCAACCCCCTCGTCTCGAGGATCA
  GTGAGAAGAGGAAGAAGCAGCTGAGGCTCTTGGAGCAGCAGGAGCTCAAGGATGAAGAGGAGGAAGAACC
  GGAAGGAAAACCCAGTGATGCAGAAAGTTCAGAGAGCTCAGATGATGAGAAAGGTTGGGTTGAAGAAGTC
  AGGAAGCAGCGCAGACTCCTTCAGCAGGAAGAAAGAGTGAAGCGGCAGGAGCAACTGAAGGAGGACCAGC
  AGACAGTCCTGAAGCCCCAGTTCTACGAGATCAAAGCAGGCGAAGAGTTCAGGAGCTTCAAAGAGTCTGC
  CACCAAGCAGAGACTAATGAACAAAACTCTGGAAGATCGTTTGAAACTTGAAGCAAAACATGGGACATTG
  AATGTATCGGACACCACTGTTGGCAGCAAGCAGTTGACATTTACTCTCAAGAGGTCTGAACAGCAAAAGA
  AGCAACAGGAGGCTGAAAAACTGCATCGTCAGGAAAGGAAAAATCTGCGCAGGTCGGCCAGCCACCTGAG
  GTCCAGACCCAGAAGAGGCCGGCCCTTTCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214070 representing NM_001008421
  Red=Cloning site Green=Tags(s)
  
  MQVSSLNEVKIYSLSCGKSLPEWLSDRKKRALQKKNVDVRRRIELIQDFEMPTVCTTIKVSKDGQYILAT
  GTYKPRVRCYDTYQLSLKFERCLDSEVVTFEILSDDYSKIVFLHNDRYIEFHSQSGFYYKTRIPKFGRDF
  SYHYPSCDLYFVGASSEVYRLNLEQGRYLNPLQTDAAENNVCDINAVHGLFATGTIEGRVECWDPRVRKR
  VGVLDCALNSVTADSEINSLPTISALKFNGALSMAVGTSTGQVLLYDLRSDKPLLVKDHQYGLPIKSVHF
  QDSLDLVLSADSRIVKMWNKDSGKIFTSLEPEHDLNDVCLYPSSGMLLTANESPKMGIYYIPVLGPAPRW
  CSFLDNLTEELEENPESTVYDDYKFVTKKDLENLGLTHLIGSPFLRAYMHGFFMDIRLYHKVKLMVNPFA
  YEEYRKDKIRQKIEETRAQRVQLKKLPKVNKELALKLIEEEEEKQKSTLKKKVKSLPNILTDDRFKVMFE
  NPDFQVDEESEEFRLLNPLVSRISEKRKKQLRLLEQQELKDEEEEEPEGKPSDAESSESSDDEKGWVEEV
  RKQRRLLQQEERVKRQEQLKEDQQTVLKPQFYEIKAGEEFRSFKESATKQRLMNKTLEDRLKLEAKHGTL
  NVSDTTVGSKQLTFTLKRSEQQKKQQEAEKLHRQERKNLRRSASHLRSRPRRGRPFH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008421
• ORF Size: 2061 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008421.1, NP_001008421.1
• RefSeq Size: 3004 bp
• RefSeq ORF: 2064 bp
• Locus ID: 217431
• UniProt ID: Q5RJG1
• Cytogenetics: 12 A1.1