Rrnad1 (NM_153562) Mouse Tagged ORF Clone

CAT#: MG214055

Rrnad1 (tGFP-tagged) - Mouse cDNA sequence BC023814 (BC023814), (10ug)

Product Images

Circular map for MG214055

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rrnad1
• Synonyms: MGC38548
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214055 representing NM_153562
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGCGTTTCAGCCCATGGCCTCTCGCATGAGGAGAGAAGGCAACTGGCTGTGGACCTCACCCGCG
  TCCTAGCTCACTACCGTTCCATCCTGGACGCCTACATCATCGAATTTTTCACGGACAGCCTGTGGGGCAC
  ACTCCCCCACTCATGGCAAGAAGCACTGGATGGACTGAACCCACCACAGCTGGCCACCCTGCTGCTGGGG
  ATGCCGAGGGACGGGGAGGAAATGAGGTACAGGTCTGTGTGGCCACTCACCCTGCTGGCCCTGAAGTCCA
  CAGCTTGTGCCCTCGCCTTTACCCGGACACCTGGCTTTCACACCCCATCAGAGTTCCTGGAGAACCCCAG
  CCAGAGCTCCCGACTAACAGCTCCTTTTCGGAAACATGTCAAGCCCAAGAAGCAGCATGAGATCCGGAGG
  CTGGGAGAGTTGGTGAAGAAGCTTAGCGATCTCACTGGCTGCACCCAAGTTGTGGATGTGGGCTCAGGCC
  AGGGCCACCTCTCCCGCTTCATGTCTCTGGGGCTGGGGCTGATGGTGAAGAGCCTTGAAGGGAATCAGAG
  GCTGGTAAAGAGAGCTCAGCATCTAGACCAGGAGCTCCTGAAGGCTCTGGACAAAATGGAGAAGAGGCAC
  CCAAAGATGGTGCAAAGAGGCCCTCGCCACCGGCCCCACCACGTGGTTCAGTGGGTCAGCCCCACAACTC
  TGTGTGAGGAGCTTCTGCTTCCTCTGGAGAGACCAGGCCAGAGTAGTGCACGTCTGCTGCTCACAGGCCT
  TCATGCTTGTGGGGATCTTAGTGTTGCCTTGCTAAGACACTTCTGCTGCTGTCCTGAAGTGGTGGCCCTG
  GCCTCGGTAGGCTGCTGCTACATGAAACTCAGTGACCCTGGCAGCTACCCACTGAGTCAGTGGATAGCTG
  GGCTGCCTGGCCATGAACTACCCTACAGGCTCCGGGAGGGTGCCTGCCATGCTCTGGAGGACTATGCGGA
  GAGATTACATAAAGCAGAGCCTGGCCTGCAAACACACTGCTTCCGTGCAGCACTGGAGACAGTCATCCGA
  CAAGTCTGCCCTGAGCTTCGGAGGCCTGGTGTGCAAGGAATCCCCAGGGTCCATGAACTCAAGATTGAAG
  AATATGTGAAACAAGGGTTAAAGCGAGTGGGTCTGGACCCCCAGCTGCCTTTGGATCTGGCTGCCCTCCA
  GGCCCACCAGGCCCAAGAGAATCGTGTGGTGGCCTTCTTCAGCTTGGCCCTCCTGCTGGCCCCACTGGTA
  GAGACATTGATTCTACTGGACCGGATGCTCTATCTTCAGGAGCAAGGCTTCTATGCTGAGCTCCTGCCCA
  TCTTCAGCCCTGAACTCTCTCCCAGAAATCTGGTTCTGGTGGCCACCAAGACGCCCCTGGGTCAGGCCTT
  CTCCACTCTTGAGACTGAAGACAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214055 representing NM_153562
  Red=Cloning site Green=Tags(s)
  
  MRGVSAHGLSHEERRQLAVDLTRVLAHYRSILDAYIIEFFTDSLWGTLPHSWQEALDGLNPPQLATLLLG
  MPRDGEEMRYRSVWPLTLLALKSTACALAFTRTPGFHTPSEFLENPSQSSRLTAPFRKHVKPKKQHEIRR
  LGELVKKLSDLTGCTQVVDVGSGQGHLSRFMSLGLGLMVKSLEGNQRLVKRAQHLDQELLKALDKMEKRH
  PKMVQRGPRHRPHHVVQWVSPTTLCEELLLPLERPGQSSARLLLTGLHACGDLSVALLRHFCCCPEVVAL
  ASVGCCYMKLSDPGSYPLSQWIAGLPGHELPYRLREGACHALEDYAERLHKAEPGLQTHCFRAALETVIR
  QVCPELRRPGVQGIPRVHELKIEEYVKQGLKRVGLDPQLPLDLAALQAHQAQENRVVAFFSLALLLAPLV
  ETLILLDRMLYLQEQGFYAELLPIFSPELSPRNLVLVATKTPLGQAFSTLETEDN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153562
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153562.4, NP_705790.3
• RefSeq Size: 2847 bp
• RefSeq ORF: 1428 bp
• Locus ID: 229503
• UniProt ID: Q8BZG5
• Cytogenetics: 3 F1