Pcdha2 (NM_198117) Mouse Tagged ORF Clone

CAT#: MG213321

Pcdha2 (tGFP-tagged) - Mouse protocadherin alpha 2 (Pcdha2), (10ug)

Product Images

Circular map for MG213321

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pcdha2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG213321 representing NM_198117
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTCTTTGAGAGGGGGCCCAGAGGTCAGGCAGTGTCTGCTGCTCTTGCTTCCTTTCCTCGCAGCCT
  GGGAGGCAGGGAACGGCCAGCTCCACTACTCCGTCCCCGAGGAGGCCAAACACGGCACTTTTGTGGGCCG
  CATCGCGCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGGGTGGCTTCCAAGGAC
  CGCGGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGG
  AGCTGTGCGGGCGGAGCGCGGAGTGTAGCATCCACCTGGAGGTGATCGTGGACAGGCCGCTGCAGGTTTT
  CCACGTGGAGGTGGAGGTGAGGGACATTAACGACAACCCGCCGCGATTTCCAATGACAGAAAAAACTATC
  CAGTTTTCCGAATCTAGGTCGCTTGACTCAAGGTTTCCTTTAGAGGGAGCCTCTGATGCAGATATCGGAG
  TAAATGCTCTTCTGTCCTACAAGCTCAGCCCCAGTGAGCTTTTCTTTCTAGATGTTCAAACAAGCGACGA
  ACTAAGCCAATCTCTGTTTCTTGTGCTGGGAAAATCTCTGGACAGAGAGGAGGATGCGGAGGTGAAATTG
  TTACTGGTAGCTACTGATGGAGGCAAGCCTGAGCTCACCGGCACTGTTCAAATACTCATCAAGGTGTTAG
  ATGTGAATGACAACGAACCTACTTTCCCCCAATCCGTTTACAAAGTACAGTTGTTAGAGAACACTGCAAA
  CGGAACCTTAGTGATGAAATTAAATGCTTCTGATGCAGATGAAGGATCAAACAGCGAGATTGTGTATTCA
  CTCGGTAGTGATGTGTCTTCCACCACACGGACTAAGTTTCAAATCGACCCCAACTCAGGAGAAGTCAGAA
  CTAAGGGAGAGTTAGATTATGAAGAAAGGACATCCTATGAAATCCAGGTCATTGCATCTGACAAGGGAAC
  CCCAGCGATGTCAGGACATTGTAAAATTTCAGTAAAACTTGTGGATATCAATGACAATACGCCAGAAGTC
  TCAATAACGTCTCTCTCACTACCCGTGCAAGAGAATGCTCCCCTGGGTGCTGTCATCGCCCTCATCTCAG
  TGTCAGATCGTGACTCGGGAGTCAATGGGCAGGTGACCTGCTCCCTGAGTCCTCATGTCCCCTTCAAGCT
  GGTGTCCACTTTCAAGAATTACTATTCTCTCGTGCTGGACAGCGCCTTGGACCGGGAAGCCACAGCTGAC
  TATAAGGTAATGGTGACAGCGCGGGATGGGGGCTCGCCCTCGCTACGGGCCACGGCCAGCGTGTCTGTGG
  AGGTGGCTGACGTGAACGACAATGCTCCAGTGTTCGCTCAGCCCGAATACACCGTGTTTGTGAAGGAGAA
  CAATCCACCAGGAGCGCACATCTTCACGGTGTCAGCGATGGATGCTGACGCACAGGAGAATGCGCTGGTG
  TCCTACTCGCTGGTGGAGCGGAGGGTGGGCGAGCGCTTGCTGTCTAGCTATGTGTCTGTGCATGCGGAGA
  GCGGCAAGGTGTTCGCTCTGCAGCCTCTGGACCATGAGGAGCTGGAGCTGCTGCAGTTTCAGGTGACTGC
  GAGGGATGCTGGTGTGCCTGCCCTGGGCAGCAATGTGACTCTGCAGGTGTTTGTGCTGGATGAGAATGAC
  AATGCACCCTTGCTGCTGCCTCAGGGAGCTGTGGGAGTTGGCGGCTCAGTGAGCGAGCTTGTACCTAGGT
  CAGTGGGTTCGGGCCATGTAGTCACCAAGGTGCGTGCCGTGGATGCGGATTCTGGTTACAACGCGTGGCT
  GTCATATGAACTGCAATCCACAGTGGGCATGCGTAGCCTGTTCCGCGTGGGTCTGTATACAGGCGAGATC
  AGCACCACGCGAGCCCTAGATGAAGTGGATGCGCCACGCCAGCGCCTGCTAGTGTTGGTGAAGGACCATG
  GTGAGCCTTCGCTGACTGCCACAGCCACAGTGTTAGTTTCTCTAGTTGAGAATAGTCAAGCTCCAAAAAC
  TTCATCAAGAGCGTTGGTAGATGCTGCTGGCCAAGAGGCGTCTCTGGTGACTGTTAACGTGTACCTGGTC
  ATCGCCATCTGCGCGGTGTCCAGCCTGTTGGTGCTCACGCTGCTGCTGTACACAGCGCTGCGCTGCTCAG
  CGATGCCCACTGATGGAGCCTGTGCTCCGGGGAGGCCCATGCTGGTGTGCTCCAGCGCGGTGGGGAGCTG
  GTCATACTCGCAGCAGAGGCGACAGAGAGTGTGCTCTGGAGAAGGCCCCCCTAAGACTGATCTCATGGCC
  TTCAGCCCTAGCCTATCCCAAGGTCCGGAATCAGCAGAAGAGAGACAACCACCCTTAGAGGCCGAATACT
  TAGGAAAGCCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAGAGCAGGCATGCACAGCTC
  TGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTA
  TCCAGTGCAACACCAGAACCTGAGGCAGGAGAGGTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACA
  GCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAGTCCGGTCCCGGTGAGTTGCCAGACAAATTCAT
  TATCCCAGGATCTCCTGCAATCATCTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGAT
  TTTATAACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGG
  AGAAAAAAGAGAAAGGGAACAGCACGACGGACAACAGTGACCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG213321 representing NM_198117
  Red=Cloning site Green=Tags(s)
  
  MPSLRGGPEVRQCLLLLLPFLAAWEAGNGQLHYSVPEEAKHGTFVGRIAQDLGLELTELVPRLFRVASKD
  RGDLLEVNLQNGILFVNSRIDREELCGRSAECSIHLEVIVDRPLQVFHVEVEVRDINDNPPRFPMTEKTI
  QFSESRSLDSRFPLEGASDADIGVNALLSYKLSPSELFFLDVQTSDELSQSLFLVLGKSLDREEDAEVKL
  LLVATDGGKPELTGTVQILIKVLDVNDNEPTFPQSVYKVQLLENTANGTLVMKLNASDADEGSNSEIVYS
  LGSDVSSTTRTKFQIDPNSGEVRTKGELDYEERTSYEIQVIASDKGTPAMSGHCKISVKLVDINDNTPEV
  SITSLSLPVQENAPLGAVIALISVSDRDSGVNGQVTCSLSPHVPFKLVSTFKNYYSLVLDSALDREATAD
  YKVMVTARDGGSPSLRATASVSVEVADVNDNAPVFAQPEYTVFVKENNPPGAHIFTVSAMDADAQENALV
  SYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVTARDAGVPALGSNVTLQVFVLDEND
  NAPLLLPQGAVGVGGSVSELVPRSVGSGHVVTKVRAVDADSGYNAWLSYELQSTVGMRSLFRVGLYTGEI
  STTRALDEVDAPRQRLLVLVKDHGEPSLTATATVLVSLVENSQAPKTSSRALVDAAGQEASLVTVNVYLV
  IAICAVSSLLVLTLLLYTALRCSAMPTDGACAPGRPMLVCSSAVGSWSYSQQRRQRVCSGEGPPKTDLMA
  FSPSLSQGPESAEERQPPLEAEYLGKPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTV
  SSATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPANNQIDKSD
  FITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198117
• ORF Size: 2844 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198117.2, NP_932785.1
• RefSeq Size: 5379 bp
• RefSeq ORF: 2847 bp
• Locus ID: 353234
• Cytogenetics: 18 B2- B3