Igsf9b (NM_001129787) Mouse Tagged ORF Clone

CAT#: MG212953

Igsf9b (tGFP-tagged) - Mouse immunoglobulin superfamily member 9B (Igsf9b) transcript variant 1, (10ug)

Product Images

Circular map for MG212953

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Igsf9b
• Synonyms: AI414108; AI854107; Gm508; mKIAA1030
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212953 representing NM_001129787
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTGGTATGTGGCCACTTTGATAGCAAGTGTGATCAGCACCCGAGGTCTTGTGGCTCAAGGTGCCC
  ACGGCCTGCGAGAGGAACCCGAGTTTGTGACTGCTCGAGCCGGCGAAGGTGTGGTTCTGCGATGCGACGT
  AATCCACCCAGTGACAGGACAGCCCCCACCCTATGTTGTAGAGTGGTTCAAGTTTGGGGTCCCCATCCCT
  ATCTTCATCAAGTTTGGCTACTATCCCCCACATGTGGACCCTGAGTATGCAGGCCGGGCCAGTCTTCATG
  ATAAAGCATCTCTGCGGCTGGAGCAGGTGCGCTCTGAGGACCAGGGTTGGTACGAGTGCAAGGTACTCAT
  GCTGGACCAGCAGTATGACACATTCCACAACGGCAGCTGGGTCCATCTCACCATTAACGCCCCTCCCACC
  TTTACAGAAACACCCCCCCAGTACATCGAGGCCAAGGAAGGTGGAAGTATTACCATGACGTGTACTGCTT
  TCGGGAACCCTAAGCCCATCGTCACCTGGCTCAAGGAAGGGACCCTCCTCGGTGCTAGTGCAAAGTATCA
  GGTGAGTGACGGTAGCCTAACGGTGACGTCAGTCAGTCGGGAGGACAGAGGCGCCTATACGTGTCGAGCA
  TATAGCATCCAGGGTGAGGCTGTGCACACAACCCATCTGCTTGTTCAAGGGCCTCCCTTCATTGTTTCCC
  CTCCTGAGAACATCACCGTCAACATCTCCCAGGATGCTCTGCTTACCTGCAGGGCAGAGGCGTATCCCGG
  CAACCTCACCTACACCTGGTACTGGCAGGATGAGAACGTCTACTTCCAGAATGACCTGAAGCTAAGGGTG
  CGGATCCTGATTGATGGGACACTGATCATCTTCCGAGTGAAGCCAGAGGATGCTGGGAAGTATACCTGTG
  TCCCTAGCAACAGCCTGGGGCGCTCCCCCTCTGCCTCAGCATACCTGACTGTGCAGTACCCAGCCCGTGT
  CCTCAACATGCCCCCTGTAATTTATGTGCCCGTGGGAATCCATGGCTATATCCGCTGTCCTGTGGATGCA
  GAGCCACCTGCTACTGTGGTGAAGTGGAATAAGGATGGCCGGCCCCTGCAGGTAGAGAAGAACTTGGGTT
  GGACCTTGATGGAGGATGGCTCTATTCGCATTGAGGAGGCCACAGAGGAGGCTCTTGGCACTTACACCTG
  TGTGCCTTACAACACCTTGGGGACTATGGGCCAGTCTGCCCCTGCACGGCTTGTCCTGAAGGACCCCCCG
  TACTTCACGGTGCTACCAGGCTGGGAATACAGGCAGGAGGCTGGCCGGGAGCTGCTCATTCCCTGTGCAG
  CTGCAGGGGACCCCTTCCCTGTCATCACCTGGAGGAAGGTAGGGAAGCCCAGCAGAAGCAAGCACAACGC
  ACTGCCCAGCGGGAGTCTCCAGTTTCGTGCCCTGAGTAAGGAGGACCACGGGGAGTGGGAATGTGTTGCC
  ACCAATGTGGTCACAAGCATCACTGCCAGCACCCACCTCACTGTCATCGGCACCAGTCCCCATGCCCCAG
  GCAGTGTCCGGGTCCATGTCTCCATGACAACTGCCAACGTGTCCTGGGAGCCGGGCTATGATGGAGGCTA
  CGAGCAGACATTCTCAGTTTGGATGAAGCGGGCTCAGTTTGGGCCCCACGACTGGCTGTCCTTGTCAGTG
  CCACCGGGCCCCAGCTGGTTGCTGGTAGACAGCCTGGAGCCTGAGACCGCATACCAGTTCAGTGTCCTGG
  CCCAGAACAGGCTGGGAACCAGCGCCTTCAGTGAGGTGGTCACTGTGAACACTTTAGCATTCCCTGTTAC
  AACTCCAGAACCCCTGGTGCTGGTGACCCCACCAAGGTGCCTCACAGCCAACCGGACCCAGCAGGGTGTG
  CTCCTGTCCTGGCTCCCACCTGCCAACCACAGCTTCCCCATCGACCGCTATATCATGGAGTTCCGAGTTG
  GGGAGCGCTGGGAGATGCTGGATGACGCCATTCCAGGCACTGACGGAGATTTTTTTGCCAAGGATCTGTC
  ACAGGATACCTGGTATGAATTCCGGGTTTTGGCTGTCATGCAGGATCTGATCAGCGAGCCCAGCAACATC
  GCCGGTGTCTCCAGCACAGACATCTTCCCGCAGCCAGACCTGACCGATGATGGGCTGGCCCGGCCAGTGT
  TGGCTGGGATAGTGGCCACCATCTGCTTCCTGGCAGCTGCCATCCTCTTCAGCACTCTGGCTGCCTGCTT
  TGTCAACAAGCAGCGCAAGCGCAAACTCAAGCGGAAGAAAGATCCTCCGCTCTCCATCACTCACTGTAGG
  AAGAGTCTTGAGTCTCCCTTGTCCTCTGGCAAGGTGAGTCCCGAGAGCATCCGCACACTCCGAGCCCCGT
  CTGAATCCTCTGACGACCAGGGCCAGCCTGCGGCCAAGAGGATGCTGAGCCCTACAAGGGAAAAAGAGCT
  GTCCCTATACAAAAAAACCAAGAGGGCTATCAGCAGCAGGAAGTATAGTGTGGCCAAGGCTGAGGCCGAA
  GCTGAGGCTACCACACCCATTGAACTGATCAGTAGAGGCCCGGATGGCCGCTTTGTCATGGGCCCATCGG
  AGATGGAGCCCTCTGTGAAGGGTCGGCGAATCGAGGGCTTCCCCTTCGCTGAGGAGACGGACATGTACCC
  TGAGTTCCGGCAGTCAGATGAGGAGAATGAAGACCCACTGGTGCCCACGTCTGTGGCTGCTCTGAAGCCT
  CAGCTGACCCCAATGTCTTCCAGCCAGGACTCTTACCTGCCACCACCAGCATACAGTCCTCGGTTCCAGC
  CTCGTGGGCTCGAGGGCCCGAGCGGACTGGGAGGACGACTCCAGGCTACTGGCCAAGCGAGGCCTCCTGC
  CCCTCGGCCCTTCCAGCACGGCCAGTATTATGGGTACCTCAGCAGCAGCAGCCCTGGGGAAGTGGAGCCT
  CCACCCTTCTATATGCCAGAAGTGGGCAGCCCCTTGAGCTCTGTCATGTCGTCCCCACCCCTGCACACCG
  AGGGGCCTTTTGGCCACCCCACCATCCCTGAGGAAAACGGAGAGAATGCTTCTAACAGCACTTTACCCTT
  GACTCAGACACCTACAGGAGGGCGCTCCCCTGAGCCCTGGGGCCGGCCAGAATTTCCCTTTGGGGGACTG
  GAGACCCCAGCTATGATGTTCCCCCACCAGCTGCACCCCTGTGATGTGGCCGAGAGTTTGCAGCCCAAGG
  CCTGCCTGCCCCGAGGACTGCCCCCAGCCCCTCTCCAGGTGCCTGCTGCCTATCCGGGCATGCTATCTCT
  GGAGGCACCAAAGGGCTGGGTAGGCAAGTCACCTGGCAGGGGTCCCATCCCGGCGCCCCCTGCCACCAAG
  TGGCAGGAAAGACCTATGCAACCTCTGGTCAGCCAAGGGCAGCTAAGACATACCAGCCAAGGTATGGGGA
  TACCGGTGTTGCCTTACCCGGAGCCAGCCGAGCCCGGGGGGCATGGTGGCCCCAGCACATTTGGCCTGGA
  TACCCGGTGGTATGAGCCCCAGCCTCGGCCCCGGCCCAGCCCCCGGCAGGCCCGGCGTGCCGAGCCCAGT
  TTACATCAAGTGGTGCTACAGCCCTCTCGGCTCTCACCTCTGACCCAAAGCCCCCTTAGTTCCCGCACTG
  GCTCCCCTGAGCTCGCTGCTCGTGCCCGACCTCGACCAGGCCTCCTGCAGCAGGCAGAGATGTCAGAGAT
  CACCCTGCAGCCGCCAGCTGCGGTCAGCTTCTCTCGAAAGTCAACACCATCATCCACGGGATCTCCTTCG
  CAGAGCAGCCGCAGTGGGAGCCCCAGCTACAGGCCCACAATGGGCTTCACTACTCTGGCCACGGGCTACC
  CTTCTCCTCCACCTGGCCCTGCCCCTCCGGCACCAGGGGACACCTTGGATGTGTTTGGACAGACGCCTTC
  CCCTCGGAGGATGGGGGAGGAGCCACTCCGGCCAGAGCCCCCAACAACCTTACCTACTTCAGGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212953 representing NM_001129787
  Red=Cloning site Green=Tags(s)
  
  MIWYVATLIASVISTRGLVAQGAHGLREEPEFVTARAGEGVVLRCDVIHPVTGQPPPYVVEWFKFGVPIP
  IFIKFGYYPPHVDPEYAGRASLHDKASLRLEQVRSEDQGWYECKVLMLDQQYDTFHNGSWVHLTINAPPT
  FTETPPQYIEAKEGGSITMTCTAFGNPKPIVTWLKEGTLLGASAKYQVSDGSLTVTSVSREDRGAYTCRA
  YSIQGEAVHTTHLLVQGPPFIVSPPENITVNISQDALLTCRAEAYPGNLTYTWYWQDENVYFQNDLKLRV
  RILIDGTLIIFRVKPEDAGKYTCVPSNSLGRSPSASAYLTVQYPARVLNMPPVIYVPVGIHGYIRCPVDA
  EPPATVVKWNKDGRPLQVEKNLGWTLMEDGSIRIEEATEEALGTYTCVPYNTLGTMGQSAPARLVLKDPP
  YFTVLPGWEYRQEAGRELLIPCAAAGDPFPVITWRKVGKPSRSKHNALPSGSLQFRALSKEDHGEWECVA
  TNVVTSITASTHLTVIGTSPHAPGSVRVHVSMTTANVSWEPGYDGGYEQTFSVWMKRAQFGPHDWLSLSV
  PPGPSWLLVDSLEPETAYQFSVLAQNRLGTSAFSEVVTVNTLAFPVTTPEPLVLVTPPRCLTANRTQQGV
  LLSWLPPANHSFPIDRYIMEFRVGERWEMLDDAIPGTDGDFFAKDLSQDTWYEFRVLAVMQDLISEPSNI
  AGVSSTDIFPQPDLTDDGLARPVLAGIVATICFLAAAILFSTLAACFVNKQRKRKLKRKKDPPLSITHCR
  KSLESPLSSGKVSPESIRTLRAPSESSDDQGQPAAKRMLSPTREKELSLYKKTKRAISSRKYSVAKAEAE
  AEATTPIELISRGPDGRFVMGPSEMEPSVKGRRIEGFPFAEETDMYPEFRQSDEENEDPLVPTSVAALKP
  QLTPMSSSQDSYLPPPAYSPRFQPRGLEGPSGLGGRLQATGQARPPAPRPFQHGQYYGYLSSSSPGEVEP
  PPFYMPEVGSPLSSVMSSPPLHTEGPFGHPTIPEENGENASNSTLPLTQTPTGGRSPEPWGRPEFPFGGL
  ETPAMMFPHQLHPCDVAESLQPKACLPRGLPPAPLQVPAAYPGMLSLEAPKGWVGKSPGRGPIPAPPATK
  WQERPMQPLVSQGQLRHTSQGMGIPVLPYPEPAEPGGHGGPSTFGLDTRWYEPQPRPRPSPRQARRAEPS
  LHQVVLQPSRLSPLTQSPLSSRTGSPELAARARPRPGLLQQAEMSEITLQPPAAVSFSRKSTPSSTGSPS
  QSSRSGSPSYRPTMGFTTLATGYPSPPPGPAPPAPGDTLDVFGQTPSPRRMGEEPLRPEPPTTLPTSG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129787
• ORF Size: 3984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129787.1, NP_001123259.1
• RefSeq Size: 4239 bp
• RefSeq ORF: 3987 bp
• Locus ID: 235086
• UniProt ID: E9PZ19
• Cytogenetics: 9 A4
• Gene Summary: Transmembrane protein which is abundantly expressed in interneurons, where it may regulate inhibitory synapse development (By similarity). May mediate homophilic cell adhesion (PubMed:23751499).[UniProtKB/Swiss-Prot Function]