Ermard (NM_001039552) Mouse Tagged ORF Clone

CAT#: MG212819

2210404J11Rik (tGFP-tagged) - Mouse RIKEN cDNA 2210404J11 gene (2210404J11Rik) transcript variant 1, (10ug)

Product Images

Circular map for MG212819

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ermard
• Synonyms: 2210404J11Rik; 2410011O22Rik; AI448938
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212819 representing NM_001039552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGATTTTACCAGGGGATTCCATGACCACATGCCTTTCTCCTTTAGTGCATGACTTGATTTGTAACC
  TTGGGTTTGAACTCAAAGAAATTTGTGATATCAATAGCATTGTAACTCAAAATGGTGAAGTACGATGGAA
  AGCAATTACTGACCGTGTGAGATATGAAGAATTAGGGCGAAGTCTGGACTACAGGAGAAGTGTGCAGCAG
  CTGGGTCCGGTGTGTGAGGCCATCCACCTACACATCTCAGCTCTGACCAGGGCTCAGTTTGAAATTCAGT
  ATTCGCCTTGGTACCAGTGGACAACTTATCCAGAGTTGTTTCTTGAGATACTTGATGCCTTGCAAAGCTC
  GCAGCCTGCTGCCGTCTCCCTCGGCGTGATGAAACTAGCTTCCTATCTGGAGCGAGCCCTCGGCGACGTA
  TTTTTACTGGTTGGGAAAGAGTGCCCCTTTCTCCTAAGAGATCTTCTTGCATCTGCAGAGCTTGCACAAG
  TCTTTGGACATGCTGTGATGGACATACTAAAAGTCTTCATCGGGTCTCCCTGCGGACTCAACCTGAGAAA
  CGTCTTGTGGCATGGCTTTGCTTCCCCTCAAGATATTCCTCCAAAATACTGTTCAGCCATGTTGCTGTTG
  ACGGCAGGACTGGGCCAGTTACTGAAGAGTTACCTTCACCACACAAAGGTCACCTTGGCACATCGACCTT
  TTGTAACACTCACAAACTTAGAGGATGTGATTGTTTTTCCTGGTGTGACTTATGAGGTACTGTCAGCATT
  AGAGACAGTGATGACAAAATCCAACTTCCTATTAAAAATCATGTTGCCATATTGGGAAATGGCAGTGAGC
  AAGTTCAAGTCGCACAGGTTTGCCGACTGCACCATGTTGTTGCTGTCACAGCTAGAAGCTGGACTCAGGA
  GACTGTTTGCTGCAGTTAACAAGTGTCCTGATAGACTGCTCACAGCCGAGTCAACAATTCTGTATACCAC
  GTTTGATGAAATACTGGCAAAACACATGAGTGATGGTAGCATGAACCAGCTTCCTTGTTTCCTTGGAGAG
  CCTGCCATGGAATTCTTGTGGGATTTCCTGAACTATCAGGAGGGTCCTCGTATCCGGGATCATTTAAGCC
  ATGGGGAGATCAACATACGTGAATTTCCAGAAGAGGCCGCAGGCCAGCTGCTCACATTCTCCCTGGTGCT
  GCTGCTCAGATTCACTGAGAAGGGGACAGTGTCAGAACTGAAGGAGGAAGCAGCAATACAGTTGCTGGTT
  AGGCTTGCAGAAGGATACAGGTCTCGCTGCCACCCAGCCTTCCAGCTTCAAAAACAGGTGCTGAGCTGTG
  AGAGAAGCCTCAGGATGTGGCCTGTGCCGCCTTTGCCAGAAGAGTGTTGTCAGGAAGCAGGCAGATTGGA
  AGGAAATTCTGAAGCATGTGCCTGCAACTCTTTAATTAGCAAGATTTGGTGTGAGCTCTGCCACTACCTG
  CCTGGGAGTGCCTGTGCTATAAATGGCTTGGATGGTCTTCCCTCAGAGAAGTGGTCCCAGCTGCTCAGTG
  AACTGTGTAGCACACGCATTCCTACACTGTTCTGCCCCAGAATCGTTCTAGAAGTTCTAGTTGTGCTCCG
  AGGCATCAACTCCCAGTGCCAACGCGTATCTGACCAGGTCACCGCCTCTTTGCAGCTAAGACACAGGCAA
  TGGGTGGAGCGTAGGTTACGTTCAAGGCAGCGTCAGAACTATGTGCGCATGCTCAGCAGTGTTAGACTTT
  TATGTCCCATGCTTTCCCTGATTTTGTTGCTCCTTGCACTGGAGTTGGCCAGTGTCCATGCTGTTCGTGA
  TAAGGGCGCTGAGGAACAACAGCAGTATCTGAGTACACTGAGAACC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212819 representing NM_001039552
  Red=Cloning site Green=Tags(s)
  
  MEILPGDSMTTCLSPLVHDLICNLGFELKEICDINSIVTQNGEVRWKAITDRVRYEELGRSLDYRRSVQQ
  LGPVCEAIHLHISALTRAQFEIQYSPWYQWTTYPELFLEILDALQSSQPAAVSLGVMKLASYLERALGDV
  FLLVGKECPFLLRDLLASAELAQVFGHAVMDILKVFIGSPCGLNLRNVLWHGFASPQDIPPKYCSAMLLL
  TAGLGQLLKSYLHHTKVTLAHRPFVTLTNLEDVIVFPGVTYEVLSALETVMTKSNFLLKIMLPYWEMAVS
  KFKSHRFADCTMLLLSQLEAGLRRLFAAVNKCPDRLLTAESTILYTTFDEILAKHMSDGSMNQLPCFLGE
  PAMEFLWDFLNYQEGPRIRDHLSHGEINIREFPEEAAGQLLTFSLVLLLRFTEKGTVSELKEEAAIQLLV
  RLAEGYRSRCHPAFQLQKQVLSCERSLRMWPVPPLPEECCQEAGRLEGNSEACACNSLISKIWCELCHYL
  PGSACAINGLDGLPSEKWSQLLSELCSTRIPTLFCPRIVLEVLVVLRGINSQCQRVSDQVTASLQLRHRQ
  WVERRLRSRQRQNYVRMLSSVRLLCPMLSLILLLLALELASVHAVRDKGAEEQQQYLSTLRT
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039552
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039552.2, NP_001034641.2
• RefSeq Size: 2513 bp
• RefSeq ORF: 1869 bp
• Locus ID: 381062
• Cytogenetics: 17 A2