Olfr452 (NM_001011869) Mouse Tagged ORF Clone

CAT#: MG212818

Olfr452 (tGFP-tagged) - Mouse olfactory receptor 452 (Olfr452), (10ug)

Product Images

Circular map for MG212818

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Olfr452
• Synonyms: MOR257-5P
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212818 representing NM_001011869
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAAGACAACCAGACATGGGTGCATGAATTCATCCTTCTTGGCCTTTCCAATGACTGGGGCACTC
  AAGTCTCCCTCTTCGTCCTGTTCTTGCTGATGTACCTGGTGACAGTATTGGGGAACTTCCTCATCATTGT
  TCTGATCAGACTGGACAGCAGACTCCACACTCCCATGTATTTCTTTCTCACCAACCTGTCCCTGGTGGAT
  GCATCTTATGCCACAAGCATAGTCCCCCAGCTGCTGGCTCATTTCCTTGCCACACACAAAGCAATTCCAT
  TTCTGAGCTGTGCAGCCCAGTTATTTTTTTCCCTGGGCTTGGGTGGGATCGAGTTCCTTCTGCTGGCAGT
  GATGGCCTATGACCGCTATGTGGCAGTGTGTGATCCTCTGAGGTATTCAGTCATCATGCATACAGGGCTC
  TGCACAAGGCTGGTCATCACATCGTGGGTCAGTGGCTCCATCAATTCTCTTGTGCACACTGCCATCACCT
  TCCAGCTGCCCATGTGCACAAATAAGTATATTGATCACATTTCCTGTGAAATTCTAGCTGTGGTCCGATT
  GGCCTGTGTGGACATTTCATCCAATGAGATTGTGATCATGGTTTCTAGCATTGTCTTGCTTATGACACCT
  TTCTTTCTGGTTCTTTTGTCCTACATTCAAATCATCTCCACCATTCTGAAAATCCAGTCTACAGAGGGAA
  GGAGGAAGGCCTTCCACACCTGTGCCTCCCACCTCACTGTGGTAACATTATGCTATGGTACCACCATTTT
  TACCTACATCCAGCCCCACTCCAGCCCCTCAGTCCTTCAAGAGAAGTTGATTTCCCTTTTTTATGCTGTA
  TTGATGCCCATGCTGAATCCTATGATTTATAGTCTAAGGAATAAGGAGGTGAAAGGGGCTTGGCAGAAAC
  TATTAGGAAAATTCTCTGTGTTCACATCAAAACTGACAAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212818 representing NM_001011869
  Red=Cloning site Green=Tags(s)
  
  MEQDNQTWVHEFILLGLSNDWGTQVSLFVLFLLMYLVTVLGNFLIIVLIRLDSRLHTPMYFFLTNLSLVD
  ASYATSIVPQLLAHFLATHKAIPFLSCAAQLFFSLGLGGIEFLLLAVMAYDRYVAVCDPLRYSVIMHTGL
  CTRLVITSWVSGSINSLVHTAITFQLPMCTNKYIDHISCEILAVVRLACVDISSNEIVIMVSSIVLLMTP
  FFLVLLSYIQIISTILKIQSTEGRRKAFHTCASHLTVVTLCYGTTIFTYIQPHSSPSVLQEKLISLFYAV
  LMPMLNPMIYSLRNKEVKGAWQKLLGKFSVFTSKLTS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011869
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011869.1, NP_001011869.1
• RefSeq Size: 954 bp
• RefSeq ORF: 954 bp
• Locus ID: 258207
• Cytogenetics: 6 B2.1
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]