Smarca2 (BC075641) Mouse Tagged ORF Clone

CAT#: MG212039

Smarca2 (tGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (cDNA clone

Product Images

Circular map for MG212039

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Smarca2
• Synonyms: brm, SNF2alpha, brahma
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212039 representing BC075641
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACACCCACAGACCCAGCAGCAATGCCCCATCCTGGGCCCTCCCCGGGGCCTGGACCCTCTCCTG
  GACCAATTCTGGGGCCTAGTCCAGGACCAGGACCATCCCCAGGTTCTGTGCACAGCATGATGGGTCCTAG
  TCCCGGACCTCCCAGCGTCTCACATCCTCTGTCAACGATGGGCTCTGCAGACTTCCCACAGGAAGGCATG
  CACCAATTACATAAGCCCATGGATGGGATACATGACAAAGGGATTGTAGAAGATGTCCACTGTGGATCCA
  TGAAGGGCACCAGCATGCGCCCCCCACACCCAGGAATGGGCCCTCCACAGAGCCCCATGGACCAGCACAG
  CCAAGGTTATATGTCACCACATCCGTCTCCTCTGGGAGCCCCGGAGCACGTCTCTAGCCCAACCCCACCC
  CAGATGCCACCGAGCCAGCCAGGGGCACTCATCCCAGGAGATCCGCAGGCCATGAACCAGCCTAACAGAG
  GTCCCTCGCCTTTCAGTCCTGTGCAGCTGCATCAGCTTCGAGCTCAGATTTTAGCTTACAAAATGTTGGC
  CAGGGGCCAGCCTCTCCCTGAAACTCTGCAGCTGGCAGTCCAGGGAAAAAGGACCTTGCCTGGCATGCAG
  CAGCAGCAGCAGCAACAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAACAAC
  AGCAGCCCCAGCAGCCTCAGCAGCAGGCTCAGGCACAGCCCCAGCAGCAGCAGCAACAGCAGCAGCAGCC
  AGCTCTTGTTAGCTATAATCGACCATCTGGCCCCGGGCAGGAGCTGCTACTGAGTGGCCAGAGCGCTCCG
  CAGAAGCTGTCAGCACCAGCACCAAGCGGCCGACCTTCACCGGCACCCCAGGCCGCCGTCCAGCCCACGG
  CCACAGCGGTGCCCGGGCCCTCCGTGCAGCAGCCCGCCCCAGGGCAGCCGTCTCCGGTCCTACAGCTGCA
  ACAGAAGCAGAGCCGCATCAGCCCCATCCAGAAACCGCAAGGCCTGGACCCGGTGGAGATCCTGCAGGAA
  CGAGAGTACAGACTTCAAGCTCGCATCGCTCATAGGATACAAGAACTGGAAAGTCTGCCTGGTTCCTTGC
  CACCAGATTTACGCACCAAAGCAACCGTGGAACTGAAAGCACTTCGCTTACTCAACTTCCAACGTCAGCT
  GAGACAGGAGGTGGTGGCCTGCATGCGGAGGGACACCACCCTGGAGACGGCCCTCAACTCCAAAGCATAT
  AAGCGGAGCAAGCGCCAGACCCTGCGTGAGGCACGCATGACAGAGAAACTGGAGAAGCAGCAGAAGATAG
  AACAGGAGAGGAAACGCCGGCAGAAACACCAGGAATACCTGAACAGTATTTTGCAACATGCAAAAGATTT
  TAAGGAATATCACCGGTCTGTGGCCGGGAAGATCCAGAAGCTCTCCAAAGCAGTGGCGACTTGGCATGCT
  AACACAGAAAGGGAGCAGAAGAAGGAGACGGAGCGGATCGAGAAGGAGAGAATGCGGAGGCTGATGGCCG
  AAGATGAAGAGGGCTACAGGAAGCTTATTGACCAAAAGAAAGACAGACGTCTCGCCTACCTATTGCAGCA
  GACCGATGAGTATGTCGCCAATCTGACCAACCTGGTGTGGGAGCACAAGCAGGCCCAAGCAGCCAAAGAG
  AAGAAGAAGAGGAGGAGGAGGAAGAAGAAGGCTGAAGAGAATGCAGAGGGAGGGGAACCTGCCCTGGGAC
  CAGATGGAGAGCCAATAGATGAAAGCAGCCAGATGAGTGACCTGCCTGTCAAAGTGACACACACAGAAAC
  TGGCAAGGTCCTCTTTGGACCAGAAGCACCCAAAGCAAGTCAGCTGGATGCCTGGCTGGAGATGAATCCT
  GGTTACGAAGTTGCACCCAGATCTGACAGTGAAGAGAGTGAATCGGACTACGAGGAGGAGGATGAAGAAG
  AAGAGTCCAGTAGGCAGGAAACCGAGGAGAAGATACTGCTGGATCCCAACAGTGAAGAAGTTTCCGAAAA
  GGATGCCAAGCAGATCATTGAGACTGCGAAGCAGGACGTGGACGACGAATACAGCATGCAGTACAGTGCC
  AGAGGCTCTCAGTCCTACTACACGGTGGCTCACGCTATCTCTGAGAGGGTGGAGAAGCAGTCTGCCCTCC
  TCATTAACGGCACCCTAAAGCATTACCAGCTCCAGGGCCTGGAATGGATGGTTTCCCTGTATAATAACAA
  TCTGAACGGAATCTTAGCTGATGAAATGGGGCTAGGCAAGACCATCCAGACCATTGCACTCATCACGTAT
  CTGATGGAGCACAAAAGGCTCAATGGTCCCTACCTCATCATCGTCCCCCTCTCGACTCTGTCTAACTGGA
  CATATGAATTTGACAAATGGGCTCCTTCTGTGGTGAAAATTTCTTACAAGGGTACCCCTGCCATGCGACG
  CTCCCTCGTTCCCCAGCTACGGAGTGGCAAATTCAATGTCCTCCTGACTACTTACGAGTACATTATAAAA
  GACAAGCACATTCTTGCAAAGATTCGGTGGAAGTACATGATCGTGGACGAAGGCCACCGGATGAAGAATC
  ACCACTGCAAGCTAACCCAGGTCCTGAACACACACTATGTGGCCCCCAGGCGGATCCTTCTGACTGGGAC
  CCCACTGCAGAATAAGCTTCCGGAACTCTGGGCCCTCCTCAACTTCCTCCTCCCTACAATCTTCAAGAGT
  TGCAGCACATTTGAGCAGTGGTTTAATGCTCCATTTGCCATGACCGGTGAAAGGGTGGACCTGAACGAAG
  AAGAAACGATTTTGATCATCAGGCGTCTACACAAGGTGCTGAGACCCTTTTTACTGAGGAGGCTGAAGAA
  AGAGGTTGAGTCTCAGCTTCCGGAAAAGGTTGAGTATGTGATCAAGTGTGACATGTCAGCTCTGCAGAAG
  ATTCTGTACCGTCACATGCAAGCCAAGGGGATCCTCCTCACGGACGGGTCTGAGAAAGATAAGAAGGGGA
  AAGGAGGTGCCAAGACACTTATGAACACCATCATGCAGCTGAGAAAAATATGCAACCACCCATATATGTT
  TCAGCACATTGAGGAATCCTTTGCTGAACACCTGGGCTATTCGAATGGGGTCATCAATGGGGCTGAGCTG
  TATCGGGCCTCGGGAAAGTTTGAGCTGCTTGATCGTATTCTGCCCAAATTGAGAGCGACTAACCACCGCG
  TGCTGCTTTTCTGCCAGATGACGTCACTCATGACCATTATGGAGGATTACTTTGCTTTTCGGAACTTCCT
  GTACCTGCGCCTTGACGGCACCACCAAGTCTGAAGATCGTGCTGCTTTGCTAAAGAAGTTCAATGAACCT
  GGGTCCCAGTATTTCATTTTCTTGCTGAGCACAAGAGCAGGGGGCCTGGGCTTAAATCTTCAGGCGGCAG
  ACACGGTGGTCATATTTGACAGCGACTGGAATCCTCACCAGGATCTGCAGGCCCAAGACCGAGCTCACCG
  CATTGGCCAACAAAACGAGGTCCGGGTGCTGAGGCTTTGCACCGTCAACAGTGTGGAGGAAAAGATTCTC
  GCGGCTGCCAAGTACAAGCTGAACGTGGATCAGAAGGTTATCCAAGCAGGCATGTTTGACCAGAAGTCAT
  CCAGCCACGAGCGGAGGGCCTTCCTGCAGGCCATTCTGGAGCACGAGGAGGAGAATGAGGAAGAAGATGA
  GGTACCAGACGACGAGACCCTGAACCAGATGATTGCTCGCCGGGAGGAAGAATTTGATCTTTTTATGCGC
  ATGGACATGGACCGGCGGAGGGAGGATGCCCGGAACCCGAAGCGCAAACCCCGCTTGATGGAGGAAGATG
  AGCTGCCCTCCTGGATTATCAAGGATGACGCCGAAGTGGAAAGGCTCACCTGTGAAGAAGAGGAGGAGAA
  GATATTTGGGAGGGGCTCTCGCCAGCGCCGGGATGTGGACTACAGTGATGCCCTCACCGAGAAGCAATGG
  CTCAGGGCCATCGAAGACGGCAATTTGGAAGAAATGGAAGAGGAGGTACGGCTTAAGAAGAGAAAAAGAC
  GAAGAAATGTGGATAAAGACCCCGTGAAGGAAGATGTGGAAAAAGCGAAGAAAAGAAGAGGCCGCCCTCC
  GGCTGAGAAGTTGTCACCAAATCCCCCAAAACTAACGAAGCAGATGAACGCCATCATTGATACTGTGATA
  AACTACAAAGACAGTTCAGGGCGACAGCTCAGTGAAGTCTTCATTCAGTTACCTTCCAGGAAAGACTTAC
  CAGAATACTATGAATTAATTAGGAAGCCAGTGGATTTCAAAAAGATAAAGGAGCGAATCCGTAATCATAA
  GTATCGGAGCCTGGGAGACCTGGAGAAAGACGTCATGCTTCTCTGTCACAACGCACAGACATTCAACTTG
  GAAGGATCCCAGATCTACGAAGACTCCATTGTCCTACAGTCAGTGTTTAAGAGTGCTCGGCAGAAAATTG
  CCAAAGAAGAAGAGAGTGAGGAAGAAAGCAATGAAGAAGAGGAAGAAGATGATGAAGAGGAGTCGGAGTC
  AGAGGCGAAATCTGTGAAGGTGAAAATCAAGCTGAATAAAAAGGAAGAGAAAGGCCGGGACACAGGGAAG
  GGCAAGAAGCGGCCAAACCGAGGCAAAGCCAAACCCGTCGTGAGCGATTTTGACAGTGACGAGGAACAGG
  AAGAGAACGAACAGTCAGAAGCAAGTGGAACTGATAACGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212039 representing BC075641
  Red=Cloning site Green=Tags(s)
  
  MSTPTDPAAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPLSTMGSADFPQEGM
  HQLHKPMDGIHDKGIVEDVHCGSMKGTSMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPTPP
  QMPPSQPGALIPGDPQAMNQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGMQ
  QQQQQQQQQQQQQQQQQQQQQQQQQPQQPQQQAQAQPQQQQQQQQQPALVSYNRPSGPGQELLLSGQSAP
  QKLSAPAPSGRPSPAPQAAVQPTATAVPGPSVQQPAPGQPSPVLQLQQKQSRISPIQKPQGLDPVEILQE
  REYRLQARIAHRIQELESLPGSLPPDLRTKATVELKALRLLNFQRQLRQEVVACMRRDTTLETALNSKAY
  KRSKRQTLREARMTEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVAGKIQKLSKAVATWHA
  NTEREQKKETERIEKERMRRLMAEDEEGYRKLIDQKKDRRLAYLLQQTDEYVANLTNLVWEHKQAQAAKE
  KKKRRRRKKKAEENAEGGEPALGPDGEPIDESSQMSDLPVKVTHTETGKVLFGPEAPKASQLDAWLEMNP
  GYEVAPRSDSEESESDYEEEDEEEESSRQETEEKILLDPNSEEVSEKDAKQIIETAKQDVDDEYSMQYSA
  RGSQSYYTVAHAISERVEKQSALLINGTLKHYQLQGLEWMVSLYNNNLNGILADEMGLGKTIQTIALITY
  LMEHKRLNGPYLIIVPLSTLSNWTYEFDKWAPSVVKISYKGTPAMRRSLVPQLRSGKFNVLLTTYEYIIK
  DKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRILLTGTPLQNKLPELWALLNFLLPTIFKS
  CSTFEQWFNAPFAMTGERVDLNEEETILIIRRLHKVLRPFLLRRLKKEVESQLPEKVEYVIKCDMSALQK
  ILYRHMQAKGILLTDGSEKDKKGKGGAKTLMNTIMQLRKICNHPYMFQHIEESFAEHLGYSNGVINGAEL
  YRASGKFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLRLDGTTKSEDRAALLKKFNEP
  GSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKIL
  AAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEENEEEDEVPDDETLNQMIARREEEFDLFMR
  MDMDRRREDARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDALTEKQW
  LRAIEDGNLEEMEEEVRLKKRKRRRNVDKDPVKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNAIIDTVI
  NYKDSSGRQLSEVFIQLPSRKDLPEYYELIRKPVDFKKIKERIRNHKYRSLGDLEKDVMLLCHNAQTFNL
  EGSQIYEDSIVLQSVFKSARQKIAKEEESEEESNEEEEEDDEEESESEAKSVKVKIKLNKKEEKGRDTGK
  GKKRPNRGKAKPVVSDFDSDEEQEENEQSEASGTDNE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC075641
• ORF Size: 4731 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC075641, AAH75641
• RefSeq Size: 5813 bp
• RefSeq ORF: 4733 bp
• Locus ID: 67155
• Cytogenetics: 19 21.17 cM
• Gene Summary: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically (PubMed:22952240, PubMed:26601204). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function]