L1cam (NM_008478) Mouse Tagged ORF Clone

CAT#: MG211864

L1cam (tGFP-tagged) - Mouse L1 cell adhesion molecule (L1cam)

Product Images

Circular map for MG211864

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: L1cam
• Synonyms: CD171; L1; N-CAM-L1; NCAM-L1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211864 representing NM_008478
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGTGATGCTGCGGTACGTGTGGCCTCTCCTCCTCTGCAGCCCCTGCCTGCTCATACAGATTCCAG
  ACGAATATAAAGGACACCATGTGCTAGAGCCACCTGTCATCACGGAACAGTCTCCACGGCGCCTGGTTGT
  CTTCCCAACAGATGACATAAGCCTGAAATGTGAAGCCAGAGGCAGACCCCAAGTGGAGTTCCGCTGGACG
  AAAGATGGCATCCACTTCAAACCCAAGGAAGAATTGGGTGTAGTGGTGCATGAGGCACCCTATTCTGGCT
  CCTTCACCATCGAAGGCAACAACAGCTTTGCCCAGAGGTTTCAGGGCATCTATCGCTGCTATGCCAGCAA
  TAAGCTAGGAACTGCCATGTCGCATGAGATCCAGCTCGTGGCCGAGGGTGCCCCCAAGTGGCCGAAGGAG
  ACTGTAAAACCTGTGGAAGTGGAGGAAGGAGAATCAGTAGTTCTGCCTTGCAACCCTCCACCCAGTGCAG
  CCCCACTTAGGATCTACTGGATGAACAGCAAGATTTTGCACATCAAACAAGATGAGCGGGTGTCCATGGG
  CCAGAATGGAGACCTATATTTTGCCAATGTGCTTACCTCAGACAATCATTCAGACTACATCTGCAATGCC
  CACTTCCCTGGTACCCGGACCATCATTCAAAAGGAACCTATTGACCTCCGGGTCAAGCCCACCAACAGCA
  TGATTGACCGGAAGCCACGTCTGCTCTTTCCCACAAACTCCAGCAGCCGCCTGGTAGCCTTGCAGGGCCA
  GTCATTGATCCTGGAGTGCATTGCTGAGGGATTCCCTACACCCACCATCAAGTGGCTGCACCCCAGTGAC
  CCAATGCCAACAGACCGTGTTATCTACCAAAACCACAACAAGACCCTGCAACTACTCAATGTGGGCGAAG
  AGGACGATGGCGAGTATACCTGCCTTGCTGAGAACTCGCTGGGCAGTGCCCGGCATGCCTACTATGTTAC
  TGTGGAAGCTGCCCCATATTGGCTGCAGAAGCCCCAGAGCCATTTGTATGGTCCAGGAGAGACTGCCCGC
  CTAGACTGCCAAGTCCAGGGCAGGCCCCAACCAGAGATCACTTGGAGAATCAACGGAATGTCTATGGAGA
  CGGTGAACAAGGACCAGAAGTACCGGATTGAGCAGGGGTCTCTGATCTTGAGTAACGTGCAGCCAAGTGA
  CACAATGGTGACCCAGTGTGAAGCCCGCAACCAGCATGGGCTCCTGCTAGCCAATGCCTACATTTATGTT
  GTCCAGCTGCCAGCCAGGATCCTAACAAAAGACAATCAGACATACATGGCAGTTGAGGGCAGTACTGCTT
  ACTTGCTGTGCAAAGCCTTTGGAGCTCCTGTTCCCAGTGTCCAGTGGCTGGATGAAGAAGGAACCACAGT
  GCTTCAGGATGAACGATTTTTCCCCTATGCCAATGGAACGCTGAGCATCAGAGACCTCCAGGCCAATGAC
  ACTGGACGCTATTTCTGCCAGGCTGCCAATGACCAGAACAATGTGACCATTTTGGCTAACCTACAGGTTA
  AAGAAGCAACCCAGATCACACAGGGGCCCCGGAGCGCAATTGAGAAGAAAGGTGCAAGGGTGACATTCAC
  GTGCCAGGCCTCCTTTGACCCCTCTTTGCAGGCCAGCATCACTTGGCGTGGAGATGGGAGAGACCTACAG
  GAACGTGGGGACAGTGACAAGTATTTCATAGAAGATGGGAAACTAGTCATCCAGAGCCTGGACTACAGTG
  ACCAGGGCAACTACAGTTGTGTGGCCAGCACTGAACTGGATGAGGTGGAGAGCAGGGCACAGCTCTTAGT
  GGTGGGGAGCCCTGGGCCAGTGCCTCACCTGGAGCTGTCCGACCGCCACCTGCTGAAGCAGAGCCAGGTG
  CACTTGTCTTGGAGCCCTGCTGAAGACCACAACTCTCCCATTGAGAAGTATGACATTGAATTTGAGGACA
  AGGAAATGGCTCCTGAGAAATGGTTCAGTCTGGGCAAGGTGCCAGGAAATCAGACCTCTACTACCCTCAA
  GCTGTCCCCCTATGTCCACTACACCTTTCGGGTCACTGCCATTAACAAATATGGTCCTGGAGAACCCAGC
  CCTGTCTCTGAGACTGTGGTCACACCTGAGGCAGCCCCAGAGAAGAACCCTGTGGATGTGAGAGGGGAAG
  GGAATGAGACCAACAATATGGTCATCACATGGAAGCCCCTTCGGTGGATGGATTGGAATGCCCCCCAGAT
  TCAGTACCGTGTACAGTGGCGTCCACAGGGCAAGCAGGAGACCTGGAGGGAACAGACCGTGAGCGACCCT
  TTCCTGGTGGTGTCTAACACTTCCACATTTGTGCCTTATGAGATCAAAGTCCAGGCAGTGAACAACCAGG
  GCAAGGGCCCTGAGCCCCAGGTCACCATTGGCTATTCAGGGGAAGACTACCCCCAGGTGAGCCCTGAACT
  TGAAGACATCACAATCTTCAACTCAAGTACTGTGCTTGTCAGGTGGAGGCCTGTGGACTTGGCCCAGGTT
  AAGGGCCACCTCAAGGGATACAATGTAACATACTGGTGGAAGGGCAGCCAGAGAAAGCACAGCAAGAGGC
  ATATCCACAAAAGCCACATAGTGGTACCTGCAAATACCACCAGTGCCATCCTCAGTGGTTTGCGCCCTTA
  CAGCTCTTACCATGTGGAGGTACAGGCCTTTAATGGGCGGGGCTTGGGGCCTGCGAGTGAATGGACCTTC
  AGCACCCCAGAGGGAGTGCCTGGCCACCCTGAGGCATTACACCTGGAGTGTCAGTCGGACACTAGTCTGC
  TACTGCACTGGCAGCCACCACTCAGCCACAATGGAGTGCTCACTGGCTACCTGCTCTCTTACCATCCCGT
  GGAAGGGGAAAGCAAAGAGCAGTTGTTCTTCAACCTTTCGGACCCAGAACTCCGGACTCATAATCTGACC
  AACCTCAACCCTGATCTACAGTACCGCTTCCAGCTTCAGGCCACCACCCAACAGGGTCCTGGTGAGGCCA
  TCGTGCGTGAAGGAGGCACCATGGCCCTGTTTGGCAAGCCAGATTTTGGCAACATCTCAGCCACAGCAGG
  TGAAAACTACAGCGTGGTCTCCTGGGTCCCTCGGAAGGGCCAGTGCAATTTCAGGTTCCATATCTTGTTC
  AAAGCCTTACCAGAAGGGAAAGTGAGCCCTGATCACCAGCCTCAGCCTCAGTATGTCAGCTACAATCAGA
  GCTCCTACACACAATGGAACCTACAGCCTGACACCAAATATGAGATCCACCTGATAAAGGAGAAGGTCCT
  CCTGCACCATCTGGATGTGAAGACTAATGGAACTGGCCCTGTGCGAGTTTCTACTACAGGTAGCTTTGCC
  TCCGAGGGCTGGTTCATCGCCTTTGTCAGCGCTATCATTCTCTTGCTCCTCATCCTGCTCATCCTCTGCT
  TCATCAAACGCAGCAAGGGTGGCAAATACTCAGTGAAGGACAAGGAGGACACTCAGGTAGATTCCGAGGC
  CCGGCCCATGAAAGACGAGACCTTCGGCGAGTACAGGTCCCTGGAGAGTGACAATGAAGAGAAGGCCTTT
  GGCAGCAGCCAGCCATCTCTCAACGGAGACATCAAACCCCTAGGCAGTGATGACAGCCTGGCTGATTATG
  GGGGCAGTGTGGACGTCCAGTTCAATGAGGATGGCTCTTTCATCGGCCAGTACAGTGGCAAGAAAGAGAA
  GGAGGCAGCAGGAGGCAATGACAGTTCAGGGGCTACCTCTCCTATCAATCCTGCAGTAGCCCTAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211864 representing NM_008478
  Red=Cloning site Green=Tags(s)
  
  MVVMLRYVWPLLLCSPCLLIQIPDEYKGHHVLEPPVITEQSPRRLVVFPTDDISLKCEARGRPQVEFRWT
  KDGIHFKPKEELGVVVHEAPYSGSFTIEGNNSFAQRFQGIYRCYASNKLGTAMSHEIQLVAEGAPKWPKE
  TVKPVEVEEGESVVLPCNPPPSAAPLRIYWMNSKILHIKQDERVSMGQNGDLYFANVLTSDNHSDYICNA
  HFPGTRTIIQKEPIDLRVKPTNSMIDRKPRLLFPTNSSSRLVALQGQSLILECIAEGFPTPTIKWLHPSD
  PMPTDRVIYQNHNKTLQLLNVGEEDDGEYTCLAENSLGSARHAYYVTVEAAPYWLQKPQSHLYGPGETAR
  LDCQVQGRPQPEITWRINGMSMETVNKDQKYRIEQGSLILSNVQPSDTMVTQCEARNQHGLLLANAYIYV
  VQLPARILTKDNQTYMAVEGSTAYLLCKAFGAPVPSVQWLDEEGTTVLQDERFFPYANGTLSIRDLQAND
  TGRYFCQAANDQNNVTILANLQVKEATQITQGPRSAIEKKGARVTFTCQASFDPSLQASITWRGDGRDLQ
  ERGDSDKYFIEDGKLVIQSLDYSDQGNYSCVASTELDEVESRAQLLVVGSPGPVPHLELSDRHLLKQSQV
  HLSWSPAEDHNSPIEKYDIEFEDKEMAPEKWFSLGKVPGNQTSTTLKLSPYVHYTFRVTAINKYGPGEPS
  PVSETVVTPEAAPEKNPVDVRGEGNETNNMVITWKPLRWMDWNAPQIQYRVQWRPQGKQETWREQTVSDP
  FLVVSNTSTFVPYEIKVQAVNNQGKGPEPQVTIGYSGEDYPQVSPELEDITIFNSSTVLVRWRPVDLAQV
  KGHLKGYNVTYWWKGSQRKHSKRHIHKSHIVVPANTTSAILSGLRPYSSYHVEVQAFNGRGLGPASEWTF
  STPEGVPGHPEALHLECQSDTSLLLHWQPPLSHNGVLTGYLLSYHPVEGESKEQLFFNLSDPELRTHNLT
  NLNPDLQYRFQLQATTQQGPGEAIVREGGTMALFGKPDFGNISATAGENYSVVSWVPRKGQCNFRFHILF
  KALPEGKVSPDHQPQPQYVSYNQSSYTQWNLQPDTKYEIHLIKEKVLLHHLDVKTNGTGPVRVSTTGSFA
  SEGWFIAFVSAIILLLLILLILCFIKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAF
  GSSQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008478
• ORF Size: 3777 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008478.3, NP_032504.3
• RefSeq Size: 5288 bp
• RefSeq ORF: 3780 bp
• Locus ID: 16728
• Cytogenetics: X 37.43 cM