Rexo1 (NM_025852) Mouse Tagged ORF Clone

CAT#: MG211807

Rexo1 (tGFP-tagged) - Mouse REX1, RNA exonuclease 1 homolog (S. cerevisiae) (Rexo1), transcript variant 1

Product Images

Circular map for MG211807

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rexo1
• Synonyms: 1700021P10Rik; 2610511M11Rik; AW050347; EloA-BP1; Rex1; Tceb3bp1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211807 representing NM_025852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTACGTTCTACCGGCTTCTTCCGCTCCATCGACTGCCCATACTGGGCGGGAGCTCCCGGGGGGCCTT
  GCCGGCGGCCCTACTGCCACTTCCGGCACCGCGGAGCTCGGGGTCCCGGCGCTCCCGGTAGCGGCGGCGC
  AGCATCTCCTGCATCAGGGCTTGGCTATGACCCGTACAACCCAGAGCTGCCCCAGCCTCCTGTGCAGAGG
  GAGAATGGTGCTCTGGGTCAGGGTGATGGCATGCTGGAGCTGGAGCTGGTCAACCAGGCCATCGAGGCTG
  TGCGCGGCGAGGTGGAGCTGGAACAGAGGCGCTACCAGGAGCTCCTCGAGACTGCACGGGGGCACAGTGC
  TGGGCCTTCAGCCCTGGCGCCTTGCAGCCCTGCCACAAGCATAGATGACGATGACACCTTCTCATTGGCC
  CTAACTTACACGCCGGGTGGCCTGCTGAGCCCGGATGCTGCCTACCAGCCCACCCCACTGGCTGTCCCTG
  CCGAGCCAGGGCACAAGTACTCACTGGCCCCTTCAGACAGGAGTCAGGGCAGAGCTGCAGGAGGTGCTGG
  TGCCCTGGAGTATGTCCCCAAGGCTGTGGGCCAGCCGCGCCGCTGTGGCCGCCCTGTGTCTGGTGGCAAG
  TATGTGGTGGATAGTTCAAAACCTTCAACAGATCTAGAGTATGACCCACTGTCCAACTACTCAGCCCGGC
  ACCTTGGCAGGGCCAGTGCTAGGGATGAGAGGGCCACCAAGAGACCGCGGGGCTCCAGGGGTGCTGAGCC
  ATACACTCCTGCCCTCAAGAAGCCCTGTGACCCCTTCGGTGGCTGTGAGGCCAGGTTCTCAGACTCAGAA
  GATGATGTGACCAGCCCCCCAAAGGCTGATGTTAGCAGCCCCAAGGCTGGAGCTGACCCTGAAAGCAAGG
  CACCTGGGAAGCCTGTCTCCAAGGAGGGCAGAGAGCATGAGGACGGTGGCCTGCGGGGCACCAAGGAGAT
  GGCTGTGCAGTATGACGTGGAAGACCTTGGGCAGCCCCCCAAAGCACCAGACACAGTGTCTGTGGTCAAG
  CCTGGCTCCCCAGCCAGGGCCTCTCAAGATGCTAGGGTCCCCAAGGAAGGTAAAGCCAAAAAGAAGAAAA
  GTGGGACATCAGCCAGCCTCAGCCACAAGGACAAGGTTCGCAAGAAAGACAAAAAGAAAGAGAAAGACCC
  AGCCAGGCCCCGAGGGAAGGAAAAGGTGTGTACGGACAAGAAGAAGCTGCCAGCCAGTAACCCCAGGGGG
  AAGGCCCAGGGGCCTGAAGGGACCAAGAAAAAGCCATCCTCTGCGACCACAGTGGCCAGCTCAGGGAAAG
  GGGGGTCAGGCCGCCCCTCCAGCACGGGACCTCAGGACTCTGGGCCTGGTCCCCATGCACCACTGGCCTG
  GAAGGCTGGTAGTGCAAAGAAGATGTCATCGGGAAAGTTGGTGGAACGCAAGGCCCGCTCCCTGGATGAA
  GGAGCCCCCCAGGACACCCCTAAGCTCAAGAAACGGGCCCTGAGCCATGCTGAACTATTTGGGGATGAGA
  GTGAGGAGGAGGACTCAAGCCTGGGAGCGGGAGCACCCCGGGTCTGGCCCCCGACCCTGCCCAGCCTTAG
  CTCAGACTCAGAATCAGACTCCGACTCCAGCCTGGGCCTGGACGAGACAAAGGTGCCCAAGCGTCTCAAG
  GCCGCGCCGCCCGCATCCCCCGTGCCGCCCTCCCCATTGTCCTCCTCCTCCTCGTCCTCGGGAGCCAGCC
  AGTGTGCAGAGGAGGATGTGGACTACTCGGCTCTGGAGAAGGAGGTGGACTTCGATGTGGACCCTATGGA
  GGAGTGCCTGCGGATCTTCAACGAGTCCACCAGCGTGAAGACAGAGGACAAGGGCCGGCTGGCCCGGCAG
  CCACCCAAGGAGAAGGCTGAGGAGAAGACACATGCGGGCCTGACCACTTTGTTCCCGGGGCAGAAGAGGA
  GGGTCTCACACCTTTGCAAGCCAGGCAAGGAGTCAGAGGCCCCAAAGAGGACCCCCGTGGCTCCCCCAGC
  ACGCCCCCCGACTGCTCAGGAGGTGTGCTACCGGCGAGCACAGCTAGCACAGAGGGACTCGGCCAGTTGG
  CTACAGGCCGCCCCGCGGCCAACAGAGAGGCTCTCCTCCGTGCACATCTCAGCGCCTGGGGAGAAGAGGA
  GGATTGCTCACGTACCCAACCCCCGACTGGCTGCCGCCCCCACAGGTGCCAAGAGGGCGCTCTCTGCCAG
  CAGCAGCCAGGCGTCCCACGGCCCCGAACCTGGCAGCCAGCCACTGAAGACACGCACATTGTCAGGGATG
  GCATCTAAGACAACCACCACCGTCACCCCCAAGCGCATCGCCCACAGCCCATCACTCCAGAGTCTAAAGA
  AGCCTGTTATCCCAAAAGAATTTGGCGGCAAGGTTCCCACTGTGGTCCGCCAGCGCTATCTCAACCTGTT
  CATTGAGGAGTGTCTCAAGTTCTGTTCGTCCAACCAGGAGGCCATCGAGAAGGCCCTGAATGAGGAAAAG
  GTGGCGTATGACCGCAGTCCCAGCAAGAACATATACCTGAACGTGGCCGTGAACACCCTTAAGAAGCTAA
  GGGGCCTGGTCCCCAACACCGTGCCCAACCTCAGCAAAGCCAGTGGCCGCAGGGTTGTGTCTCATGAGGT
  TGTACTGGGGGGCAAATTGGCAGCCAAAACCAGCTTCTCATTGAGCCGCCCCAGCAGTCCGCGGGTAGAG
  GAGCTGAAAGGGACTGCCCTGTACAGCCGCCTCAGGGAGTACCTGCTCACTCAGGAGCAGCTGAAGGAGA
  ATGGCTACCCCTTCCCCCACCCAGAGCGCCCAGGGGGTGCCATCATCTTCACAGCCGAGGAGAAGAAACC
  CAAGGACCCCTCCTGCAGAATTTGCTGTCGCTGTGGCACAGAGTACCTTGTGTCCTCCTCGGGCCGCTGC
  GTGCGCAGTGAGGAGTGTTACTACCACTGGGGGCGGCTGCGGCGGAATCGAGTGGCTGGGGGCTGGGAGA
  CCCAGTATATGTGCTGCTCAGCTGCCGTCGGCTCTGTAGGCTGTCAGGTTGCCAAGCAACACGTGCAGGA
  CGGCCGGAAGGAGAACCTGGAGGGCTTTGTGCGCACCTTCCAGAAGGAGCTGCCTGAAGATGCCCATGCA
  GGGGTCTTTGCCCTCGACTGTGAGATGTCCTACACTACATATGGCCTGGAGCTGACCCGAGTCACAGTTG
  TGGACACAGACATGCAGGTCGTGTATGACACCTTTGTCAAGCCTGACAATGAAGTTGTTGACTACAACAC
  CAGGTTCTCAGGGGTGACAGAGGCTGACCTTGTGGACACGAGCATCACGCTGCGGGACGTGCAGGCTGTC
  TTGCTGAGCATGTTCAGCGCCGACACCATCCTCATCGGACACAGCCTGGAGAGCGACCTGCTGGCTCTCA
  AGGTCATCCACGGCACAGTGGTGGACACGTCTGTGCTTTTCCCACATCGCCTGGGCCTGCCCTATAAGCG
  CTCCCTGCGGAACCTCATGGCTGACTACCTCAGACAGATCATCCAAGACAATGTGGACGGGCACAGCTCC
  AGTGAGGATGCCAGTGCCTGCATGCACCTTGTCATCTGGAAGATCCGAGAAGATGCCAAGACCAAGCGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211807 representing NM_025852
  Red=Cloning site Green=Tags(s)
  
  MLRSTGFFRSIDCPYWAGAPGGPCRRPYCHFRHRGARGPGAPGSGGAASPASGLGYDPYNPELPQPPVQR
  ENGALGQGDGMLELELVNQAIEAVRGEVELEQRRYQELLETARGHSAGPSALAPCSPATSIDDDDTFSLA
  LTYTPGGLLSPDAAYQPTPLAVPAEPGHKYSLAPSDRSQGRAAGGAGALEYVPKAVGQPRRCGRPVSGGK
  YVVDSSKPSTDLEYDPLSNYSARHLGRASARDERATKRPRGSRGAEPYTPALKKPCDPFGGCEARFSDSE
  DDVTSPPKADVSSPKAGADPESKAPGKPVSKEGREHEDGGLRGTKEMAVQYDVEDLGQPPKAPDTVSVVK
  PGSPARASQDARVPKEGKAKKKKSGTSASLSHKDKVRKKDKKKEKDPARPRGKEKVCTDKKKLPASNPRG
  KAQGPEGTKKKPSSATTVASSGKGGSGRPSSTGPQDSGPGPHAPLAWKAGSAKKMSSGKLVERKARSLDE
  GAPQDTPKLKKRALSHAELFGDESEEEDSSLGAGAPRVWPPTLPSLSSDSESDSDSSLGLDETKVPKRLK
  AAPPASPVPPSPLSSSSSSSGASQCAEEDVDYSALEKEVDFDVDPMEECLRIFNESTSVKTEDKGRLARQ
  PPKEKAEEKTHAGLTTLFPGQKRRVSHLCKPGKESEAPKRTPVAPPARPPTAQEVCYRRAQLAQRDSASW
  LQAAPRPTERLSSVHISAPGEKRRIAHVPNPRLAAAPTGAKRALSASSSQASHGPEPGSQPLKTRTLSGM
  ASKTTTTVTPKRIAHSPSLQSLKKPVIPKEFGGKVPTVVRQRYLNLFIEECLKFCSSNQEAIEKALNEEK
  VAYDRSPSKNIYLNVAVNTLKKLRGLVPNTVPNLSKASGRRVVSHEVVLGGKLAAKTSFSLSRPSSPRVE
  ELKGTALYSRLREYLLTQEQLKENGYPFPHPERPGGAIIFTAEEKKPKDPSCRICCRCGTEYLVSSSGRC
  VRSEECYYHWGRLRRNRVAGGWETQYMCCSAAVGSVGCQVAKQHVQDGRKENLEGFVRTFQKELPEDAHA
  GVFALDCEMSYTTYGLELTRVTVVDTDMQVVYDTFVKPDNEVVDYNTRFSGVTEADLVDTSITLRDVQAV
  LLSMFSADTILIGHSLESDLLALKVIHGTVVDTSVLFPHRLGLPYKRSLRNLMADYLRQIIQDNVDGHSS
  SEDASACMHLVIWKIREDAKTKR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025852
• ORF Size: 3639 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025852.3, NP_080128.2
• RefSeq Size: 5249 bp
• RefSeq ORF: 3642 bp
• Locus ID: 66932
• UniProt ID: Q7TT28
• Cytogenetics: 10 C1
• Gene Summary: Seems to have no detectable effect on transcription elongation in vitro.[UniProtKB/Swiss-Prot Function]