Nrip1 (NM_173440) Mouse Tagged ORF Clone

CAT#: MG211726

Nrip1 (tGFP-tagged) - Mouse nuclear receptor interacting protein 1 (Nrip1)

Product Images

Circular map for MG211726

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nrip1
• Synonyms: 6030458L20Rik; 8430438I05Rik; 9630050P12; AA959574; AW456757; RIP140
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211726 representing NM_173440
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCATGGAGAAGAGCTTGGCTCTGATGTGCATCAGGATTCTATTGTCTTAACTTACCTCGAAGGGT
  TACTAATGCATCAGGCAGCAGGGGGATCAGGCACTGCCATTAACAAAAAGTCTGCTGGCCACAAAGAGGA
  AGACCAGAACTTTAACCTCTCGGGCAGTGCGTTTCCCTCCTGTCAAAGCAATGGTCCCACTGTCAGTACC
  CAGACGTACCAGGGATCTGGCATGCTGCACCTCAAAAAAGCCAGACTGCTGCAGTCTTCCGAGGACTGGA
  ACGCGGCAAAGCGGAAGAGGCTGTCTGATTCCATCGTGAATTTAAACGTAAAGAAGGAAGCGTTGCTGGC
  TGGCATGGTTGACAGTGTGCCTAAAGGCAAACAGGATAGCACATTGCTGGCCTCTTTGCTTCAGTCATTC
  AGCTCTAGGCTGCAGACTGTTGCTCTGTCACAGCAGATTAGACAGAGCCTCAAGGAGCAGGGATATGCCC
  TCAGTCACGAGTCTTTAAAAGTGGAGAAGGATTTAAGGTGCTATGGCGTGGCCTCAAGTCACTTAAAAAC
  TCTGTTGAAGAAAAGTAAAACCAAGGATCAAAAGTCAGGTCCCACCCTCCCTGACGTGACTCCAAACCTT
  ATCAGAGATAGCTTTGTTGAGTCATCCCATCCCGCAGTGGGACAAAGTGGGACAAAGGTCATGAGTGAGC
  CCTTGTCATGTGCTGCAAGATTACAGGCTGTTGCCAGCATGGTGGAGAAAAGGGCGAGTCCCGCTGCCTC
  CCCAAAGCCTAGTGTTGCCTGCAGCCAGTTGGCGCTGCTCCTGTCCAGCGAGGCCCACCTGCAGCAGTAC
  TCTCGGGAACATGCTCTAAAAACGCAGAACGCACATCAGGTGGCAAGCGAAAGACTTGCAGCCATGGCCA
  GATTGCAAGAGAATGGGCAGAAGGACGTGGGCAGTTCGCAGCTCTCCAAAGGGGTGTCTGGCCATCTCAA
  CGGGCAGGCCAGAGCACTGCCGGCAAGCAAACTGGTGGCCAACAAGAATAACGCTGCCACCTTTCAGAGT
  CCAATGGGTGTTGTCCCTTCCTCCCCCAAAAACACGAGCTATAAGAACTCACTGGAAAGAAACAACCTAA
  AGCAGGCTGCTAATAACAGTCTGCTTTTGCATCTCCTCAAAAGCCAGACCATACCCACGCCGATGAACGG
  GCACAGCCAGAACGAGAGAGCGAGCAGTTTTGAGAGTAGCACGCCCACCACGATTGATGAGTACTCCGAT
  AACAACCCGAGCTTTACAGATGACAGCAGTGGAGACGAAAGCTCGTACTCCAATTGCGTTCCCATAGACC
  TGTCTTGCAAACACCGGATCGAAAAGCCGGAAGCTGAGCGGCCCGTTTCGCTGGAGAACCTAACCCAGTC
  CTTGTTAAACACGTGGGATCCCAAGATCCCCGGCGTTGACATCAAAGAAGATCAAGATACCTCAACAAAT
  TCCAAGCTGAATTCACACCAGAAAGTCACTCTTCTTCAGTTGCTGCTCGGCCATAAAAGTGAAGAAACTG
  TTGAAAGGAACGCCAGCCCTCAGGACATCCATAGTGATGGGACTAAGTTCAGTCCTCAGAATTACACAAG
  GACTTCTGTCATCGAAAGCCCCAGTACCAACAGGACTACCCCAGTGAGCACTCCACCACTGTATACAGCC
  AGCCAAGCAGAGTCTCCCATCAATCTTTCCCAGCACTCTCTGGTCATCAAGTGGAATTCCCCGCCGTATG
  CCTGCAGTACTCCCGCTTCCAAGCTCACGAACACCGCGCCTAGCCACCTGATGGACCTCACGAAAGGCAA
  AGAGTCCCAAGCCGAGAAACCAGCCCCGAGTGAAGGTGCACAAAATTCCGCCACGTTCAGTGCCAGTAAA
  CTGTTACAAAATTTGGCTCAGTGCGGATTGCAGTCTTCCGGGCCAGGGGAAGAGCAGAGACCCTGCAAAC
  AGCTGTTAAGTGGAAACCCAGACAAACCTCTCGGTCTGATTGATAGATTAAACAGCCCTCTGCTCTCAAA
  TAAAACCAATGCGGCTGAAGAGAGCAAAGCCTTCAGCAGTCAGCCTGCCGGGCCTGAGCCGGGACTTCCT
  GGTTGTGAGATAGAAAATCTCTTGGAAAGACGGACTGTCCTTCAGTTGCTCCTGGGAAATTCCAGCAAAG
  GGAAGAATGAGAAGAAAGAGAAAACCCCCGCACGAGACGAGGCTCCTCAGGAGCATTCGGAGAGGGCTGC
  AAATGAACAGATACTCATGGTGAAGATTAAATCCGAGCCTTGTGACGACTTCCAGACCCACAACACAAAC
  CTGCCCTTAAACCACGATGCCAAGAGCGCCCCCTTCTTAGGTGTGACTCCCGCCATCCACAGGAGCACAG
  CGGCCTTACCAGTGTCGGAGGACTTTAAATCCGAGCCTGCTTCACCTCAGGATTTCTCTTTCTCAAAGAA
  CGGGCTGTTGAGTCGCTTGCTGAGACAGAATCAAGAGAGTTACCCGGCAGATGAGCAGGACAAGAGTCAC
  AGAAACAGTGAGCTGCCAACCCTGGAGTCGAAGAACATCTGCATGGTCCCGAAGAAAAGGAAGCTGTATA
  CGGAACCACTGGAGAATCCATTTAAAAAGATGAAAAATACTGCCGTAGATACTGCCAATCATCACAGCGG
  CCCGGAAGTACTCTACGGGTCGTTGCTTCATCAGGAAGAGCTGAAGTTTAGCAGGAATGAGCTCGATTAT
  AAATACCCTGCTGGGCATAGTTCAGCCAGCGATGGTGACCACAGGAGTTGGGCCAGAGAGAGCAAAAGCT
  TCAATGTTCTCAAGCAGCTGCTGCTCTCCGAGAACTGTGTGCGAGATCTGTCCCCACACAGGAGTGACTC
  TGTCCCCGACACGAAAAAGAAAGGACACAAAAACAACGCGCCCGGCAGCAAACCTGAATTCGGCATTTCT
  TCTTTAAATGGACTGATGTATAGTTCCCCGCAGCCTGGCAGTTGTGTGACGGATCATAGGACATTTTCAT
  ACCCGGGAATGGTAAAGACCCCTCTGAGCCCTCCTTTCCCAGAGCACTTGGGCTGTGTGGGGTCCAGACC
  AGAACCTGGGCTTTTGAATGGATGTTCCGTGCCCGGTGAGAAGGGACCCATTAAGTGGGTCATCGCAGAT
  ATGGATAAGAATGAATACGAAAAAGACTCTCCAAGACTGACCAAAACTAATCCGATCCTCTATTACATGC
  TCCAGAAGGGAGGGGGCAATTCTGTTACCACACAAGAAACCCAGGACAAAGACATCTGGAGGGAGCCTGC
  GTCAGCCGAGAGTCTCTCACAGGTTACAGTCAAAGAAGAGCTACTTCCCGCTGCAGAAACTAAAGCTTCT
  TTCTTTAATCTAAGAAGCCCGTACAATAGCCATATGGGAAATAATGCTTCTCGCCCACACAGTACAAATG
  GAGAAGTGTATGGACTTCTGGGAAACGCGCTCACCATAAAAAAAGAGTCAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211726 representing NM_173440
  Red=Cloning site Green=Tags(s)
  
  MTHGEELGSDVHQDSIVLTYLEGLLMHQAAGGSGTAINKKSAGHKEEDQNFNLSGSAFPSCQSNGPTVST
  QTYQGSGMLHLKKARLLQSSEDWNAAKRKRLSDSIVNLNVKKEALLAGMVDSVPKGKQDSTLLASLLQSF
  SSRLQTVALSQQIRQSLKEQGYALSHESLKVEKDLRCYGVASSHLKTLLKKSKTKDQKSGPTLPDVTPNL
  IRDSFVESSHPAVGQSGTKVMSEPLSCAARLQAVASMVEKRASPAASPKPSVACSQLALLLSSEAHLQQY
  SREHALKTQNAHQVASERLAAMARLQENGQKDVGSSQLSKGVSGHLNGQARALPASKLVANKNNAATFQS
  PMGVVPSSPKNTSYKNSLERNNLKQAANNSLLLHLLKSQTIPTPMNGHSQNERASSFESSTPTTIDEYSD
  NNPSFTDDSSGDESSYSNCVPIDLSCKHRIEKPEAERPVSLENLTQSLLNTWDPKIPGVDIKEDQDTSTN
  SKLNSHQKVTLLQLLLGHKSEETVERNASPQDIHSDGTKFSPQNYTRTSVIESPSTNRTTPVSTPPLYTA
  SQAESPINLSQHSLVIKWNSPPYACSTPASKLTNTAPSHLMDLTKGKESQAEKPAPSEGAQNSATFSASK
  LLQNLAQCGLQSSGPGEEQRPCKQLLSGNPDKPLGLIDRLNSPLLSNKTNAAEESKAFSSQPAGPEPGLP
  GCEIENLLERRTVLQLLLGNSSKGKNEKKEKTPARDEAPQEHSERAANEQILMVKIKSEPCDDFQTHNTN
  LPLNHDAKSAPFLGVTPAIHRSTAALPVSEDFKSEPASPQDFSFSKNGLLSRLLRQNQESYPADEQDKSH
  RNSELPTLESKNICMVPKKRKLYTEPLENPFKKMKNTAVDTANHHSGPEVLYGSLLHQEELKFSRNELDY
  KYPAGHSSASDGDHRSWARESKSFNVLKQLLLSENCVRDLSPHRSDSVPDTKKKGHKNNAPGSKPEFGIS
  SLNGLMYSSPQPGSCVTDHRTFSYPGMVKTPLSPPFPEHLGCVGSRPEPGLLNGCSVPGEKGPIKWVIAD
  MDKNEYEKDSPRLTKTNPILYYMLQKGGGNSVTTQETQDKDIWREPASAESLSQVTVKEELLPAAETKAS
  FFNLRSPYNSHMGNNASRPHSTNGEVYGLLGNALTIKKESE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173440
• ORF Size: 3483 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173440.3
• RefSeq Size: 4529 bp
• RefSeq ORF: 3486 bp
• Locus ID: 268903
• UniProt ID: Q8CBD1
• Cytogenetics: 16 C3.1
• Gene Summary: Modulates transcriptional repression by nuclear hormone receptors such as NR2C1, thyroid hormone receptor and retinoic acid receptor/RARA. Essential for cumulus expansion and follicle rupture during ovulation. Also controls the balance between fat accumulation and energy expenditure. Positive regulator of the circadian clock gene expression: stimulates transcription of ARNTL/BMAL1, CLOCK and CRY1 by acting as a coactivator for RORA and RORC.[UniProtKB/Swiss-Prot Function]