Synrg (NM_194341) Mouse Tagged ORF Clone

CAT#: MG211695

Synrg (tGFP-tagged) - Mouse AP1 gamma subunit binding protein 1 (Ap1gbp1)

Product Images

Circular map for MG211695

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Synrg
• Synonyms: Ap1gbp1; SYNG
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211695 representing NM_194341
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCGGCCGGGAGCCGGAGCCAGCGGCGCGGCGGGGGCGGGCGCGGGGCCCGGCGGGGCAGGCA
  GCTTCATGTTTCCTGTTGCAGGCGGAATGAGACCCCCTCAAGGCCTGATTCCAATGCAACAGCAAGGATT
  CCCTATGGTCTCTGTCATGCAGCCCAATATGCAAGGCATGATGGGAATGAATTACAGCTCTCAGATGTCC
  CAAGGACCGATTGCAATGCAGGCAGGAATACCAATGGGGCCAATGCCAGCAGCGGGTGTGCCTTTCCTGG
  GTCAGCCACCTTTCTTGAGCATGCGTCCTGCAGGCCCACAGTACACTCCAGACATGCAGAAGCAGTTTGC
  GGAAGAGCAACAAAAACGATTTGAACAACAACAAAAGCTCTTAGAAGAAGAAAGAAAAAGACGTCAGTTT
  GAAGAGCAAAAGCAAAAGCTCAGACTTCTCAGCAGCGTGAAGCCCAAGACAGGAGAGAAGAACAGAGACG
  ATGCTTTGGAAGCCATAAAAGGAAACTTGGATGGGTTTTCCAGAGATGCTAAGATGCACCCCACTCCAGC
  ATCACATCCAAAGAAACAAGGTGTGGGCGTGTTTCCCTCTCAGGACCCTGCTCAGTCCAGAATGCCTCCT
  TGGATTTACAATGAGAGTTTGGTTCCAGATGCCTATAAGAAAATTTTAGAAACCACAATGACTCCAACTG
  GAATAGATACTGCTAAACTTTATCCCATTCTGATGTCATCTGGACTTCCCAGGGAAACTCTCGGACAGAT
  ATGGGCCTTAGCTAATCGAACCACACCTGGCAGGCTCACTAAGGAGGAGCTTTACACGGTTCTCGCCATG
  GTAGCAGTAACACAGAGGGGTGTTCCTGCCATGAGCCCTGATGCTTTGAGCCAGTTCCCTGCGGCTCCCA
  TCCCTACCTTAAGCGGCTTTCCTATGACTCTGCCTACCCCAGTGAGCCAGCCAACGGCGATGCCCTCTGG
  CCCTACAGGCTCCATGCCCCTCACCCTTGGACAACCCATCATGGGTATCAACCTTGTTGGACCAGTGGGT
  GGAGCTGCAGCACCAACTTCCAGTGGCTTCATGCCAGCCTACCCATCAAACCAGGTGGGAAAGACAGAAG
  AAGATGACTTCCAGGATTTTCAAGATGCTTCGAAATCAGGATCCATCGATGATTCCTTCACTGACTTCCA
  AGAGATGCCTGCTTCCTCAAAAACCAGTAATTCCCAGCATGGAAACAGTGCTCCATCACTCTTGATCCCA
  TTTCCTGGAACTAAAGCCTCCACAGACAAATACGCAGTGTTCAAAGGGATTTCCACTGACAAGCCCTCTG
  AAAACCCTGCTTCGTTTGGAGAGTCTGGTGATAAATACAGTGCTTTCAGGGAACTCGAGCAAACGACAGA
  CAGTAAACCTTTAGGAGAGAGCTTTGCCGAGTTCAGATCCACAGGAACCGATGATGGCTTCACTGACTTC
  AAGACCGCCGATAGTGTGTCACCCCTAGAGCCACCCACAAAAGACACTTTCCCATCAGCTTTTGCCTCAG
  GAGCTGCACAGCAGACACAGACACAGGTGAAAACCCCTCTGAACTTAGAAGATCTAGATATGTTCTCCTC
  AGTGGATTGCAGTGGCGAGAAACAGGTGCCCTTTTCAGCTACATTTAGCACAGCAAAGTCCGTTTCCACT
  CGACCTCAGCCAGCGGGCTCTGCAGCAGCGTCAGCAGCACTGGCATCCACTAAAACTTCTAGTTTGGCAG
  ATGATTTTGGAGAGTTCAACCTCTTTGGGGAGTATTCAAACCCTGCATCTGCTGGGGAGCAGGACGACTT
  TGCAGATTTTATGGCTTTCGGTAACAGCTCCATTTCATCTGAGCCAAAAGCAAGTGACAAGTATGAAGCC
  CTCAGGGAGGAAGTGAGTCCCAGCCCCTTGTCCAGCAGCACTGTGGAAGGGGCACAGCACCCACCTGCTG
  CGGCAACCAAGTATGATGTCTTCAAACAGCTGTCCCTAGAGGGCGCTGGGCTGGCCATGGAGGAGTTCAA
  AGAGAACACTTCCTCCACCAAGAGTGAAGATGACTTTGCCGACTTCCACTCCAGTAAATTCTCATCCACG
  AGCTCAGACAAATCCCTGGGAGAGAAAGCCGTGGCTTTCAGACATGCCAAAGAAGACTCCTCCTCGGTGA
  AGTCTTTAGACCTCCCCTCCATCGGTGGCAGCAGTGTTGGCAAGGAGGACTCTGAAGATGCACTTTCTGT
  TCAATTTGACATGAAATTGGCTGATGTGGGAGGAGATCTTAAGCATGTCATGTCTGATAGCTCTTTGGAT
  TTACCAACAGTTAGTGGCCAGCATCCTCCTGCCGCAGGATCGGCCTTGGCCAGTGAGGACGCTCTTCCAG
  AGACTGCCTTCCCAGCTTTTGCCAGCTTTAAAGACATGATGCCTCAGACCACTGAGCAGAAAGAGTTTGA
  AAGTGGGGACTTCCAGGATTTCACCAGGCAGGACATGCCCACGGTGGACCGCAGCCAAGAAACCTCATGT
  CCAAGCCCGGCTTCCAGTGTGGCTTCGCACGAGACCCCCAAGGAGGGTGCAGACGACTTTGGAGAATTTC
  AAAGTGAAAAGTCCAAAATCAGCAAGTTTGACTTCTTAGTAGCTAATTCCCAGAGCAAAATGAAATCCAG
  TGAAGAAATGATCAAAAGTGAGCTGGCAACCTTTGACCTTTCTGTTCAAGGATCACACAAGAGGAGCTTG
  AGCCTTGGGGATAAAGAAATAAGCCGGTCCTCTCCTTCCCCGGCCCTGGAGCAGCCCTTCAGAGACCGGT
  CCAACACTCTGAGTGAGAGGGCTGCGCTGCCCGTCATCCGAGACAAGTACAAGGATCTCACTGGCGAGGT
  GGAGGAAAATGAGAGATACGCGTATGAGTGGCAGAGATGCCTAGGGAGCGCGCTGGATGTCATTAAGAAG
  GCAAATGACACCTTAAATGGCATTAGCAGCAGTGCTGTTTGCACAGAAGTTATCCAGTCGGCTCAAGGCA
  TGGAGTATCTTCTAGGTGTGGTGGAAGTGTACCGCGTAACCAAGCGTGTGGAGCTGGGGATAAAAGCCAC
  TGCTGTGTGTAGTGAGAAACTCCAGCAGTTGCTGAAGGACATCGATAAAGTGTGGAACAACTTAATCGGC
  TTCATGTCACTTGCCACACTCACACCAGATGAGAATTCACTGGATTTTTCCTCCTGTATGTTACGGCCCG
  GGATCAAAAACGCTCAGGAGCTGGCTTGTGGAGTGTGTCTCTTAAATGTGGACTCCAGGAGCCGGGCATT
  CAACTCGGAGACAGACAGCTTCAAGCTGGCCTACGGAGGACACCAGTACCACGCCAGCTGTGCCAACTTC
  TGGATCAACTGTGTTGAACCCAAGCCTCCCGGCCTCCTCTTGCCAGATCTGCTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211695 representing NM_194341
  Red=Cloning site Green=Tags(s)
  
  MALRPGAGASGAAGAGAGPGGAGSFMFPVAGGMRPPQGLIPMQQQGFPMVSVMQPNMQGMMGMNYSSQMS
  QGPIAMQAGIPMGPMPAAGVPFLGQPPFLSMRPAGPQYTPDMQKQFAEEQQKRFEQQQKLLEEERKRRQF
  EEQKQKLRLLSSVKPKTGEKNRDDALEAIKGNLDGFSRDAKMHPTPASHPKKQGVGVFPSQDPAQSRMPP
  WIYNESLVPDAYKKILETTMTPTGIDTAKLYPILMSSGLPRETLGQIWALANRTTPGRLTKEELYTVLAM
  VAVTQRGVPAMSPDALSQFPAAPIPTLSGFPMTLPTPVSQPTAMPSGPTGSMPLTLGQPIMGINLVGPVG
  GAAAPTSSGFMPAYPSNQVGKTEEDDFQDFQDASKSGSIDDSFTDFQEMPASSKTSNSQHGNSAPSLLIP
  FPGTKASTDKYAVFKGISTDKPSENPASFGESGDKYSAFRELEQTTDSKPLGESFAEFRSTGTDDGFTDF
  KTADSVSPLEPPTKDTFPSAFASGAAQQTQTQVKTPLNLEDLDMFSSVDCSGEKQVPFSATFSTAKSVST
  RPQPAGSAAASAALASTKTSSLADDFGEFNLFGEYSNPASAGEQDDFADFMAFGNSSISSEPKASDKYEA
  LREEVSPSPLSSSTVEGAQHPPAAATKYDVFKQLSLEGAGLAMEEFKENTSSTKSEDDFADFHSSKFSST
  SSDKSLGEKAVAFRHAKEDSSSVKSLDLPSIGGSSVGKEDSEDALSVQFDMKLADVGGDLKHVMSDSSLD
  LPTVSGQHPPAAGSALASEDALPETAFPAFASFKDMMPQTTEQKEFESGDFQDFTRQDMPTVDRSQETSC
  PSPASSVASHETPKEGADDFGEFQSEKSKISKFDFLVANSQSKMKSSEEMIKSELATFDLSVQGSHKRSL
  SLGDKEISRSSPSPALEQPFRDRSNTLSERAALPVIRDKYKDLTGEVEENERYAYEWQRCLGSALDVIKK
  ANDTLNGISSSAVCTEVIQSAQGMEYLLGVVEVYRVTKRVELGIKATAVCSEKLQQLLKDIDKVWNNLIG
  FMSLATLTPDENSLDFSSCMLRPGIKNAQELACGVCLLNVDSRSRAFNSETDSFKLAYGGHQYHASCANF
  WINCVEPKPPGLLLPDLL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194341
• ORF Size: 3414 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194341.2
• RefSeq Size: 4589 bp
• RefSeq ORF: 3417 bp
• Locus ID: 217030
• UniProt ID: Q5SV85
• Cytogenetics: 11 C
• Gene Summary: May play a role in endocytosis and/or membrane trafficking at the trans-Golgi network (TGN). May act by linking the adapter protein complex AP-1 to other proteins (By similarity).[UniProtKB/Swiss-Prot Function]