Rfc1 (NM_011258) Mouse Tagged ORF Clone

CAT#: MG211679

Rfc1 (tGFP-tagged) - Mouse replication factor C (activator 1) 1 (Rfc1)

Product Images

Circular map for MG211679

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rfc1
• Synonyms: 140kDa; Alp145; Recc1; RFC140
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211679 representing NM_011258
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACATTCGGAAATTCTTTGGGGTTATATCAAGTGGAAAAAAGCCTGTAAACGAGACAGTAAAGAATG
  AGAAGACAAAAGCTTCGGAGGGAACTGTCAAAGGAAAAAAGGGAGTCAAGGAGGCCAAGGTCAATAACTC
  CGGTAAAGAGGACGCCTCCAAACCAAAGCAGCACAGCAAGAAGAAGAGGATCATCAATGACTCAGACTCA
  GAGTCAGAGGAGACAGTACAGGTAAAAAATGCTAAAAAGAAATCAGAAAAATTGTCACTGTCTTATAAAC
  CTGGTAAAGTCTCTCAGAAGGATCCTGTCACCTACGTCTCTGAGACAGATGAAGATGATGACTTTGTATG
  TAAAAAGGCAGCCTCCAAATCAAAAGAGAATGGAGTATCTACAAATAGTTACCTTGGAACATCAAACGTG
  AAAAAAAATGAAGAAAACGTTAAGACTAAGAACAAGCCGTTATCTCCAATAAAACTCACACCAACGTCAG
  TGCTCGACTATTTTGGAACTGAAAGTGTCCAGAGATCTGGGAAGAAGATGGTGACAAGCAAAAGGAAAGA
  ATCTTCTCAAAACACAGAGGATTCCAGATTAAATGATGAGGCCATCGCCAAGCAGCTGCAGCTGGATGAA
  GATGCAGAGCTGGAGAGGCAGTTGCATGAAGATGAAGAATTTGCAAGAACACTGGCCTTATTGGATGAAG
  AACCCAAGATCAAAAAGGCCCGAAAGGATTCTGAAGAGGGAGAAGAATCATTTTCATCTGTCCAAGATGA
  TTTAAGCAAAGCAGAAAAGCAGAAAAGCCCTAATAAAGCCGAGCTTTTCTCAACTGCAAGAAAGACCTAC
  AGTCCTGCTAAGCATGGCAAGGGGAGGGCTTCAGAAGATGCTAAGCAGCCTTGCAAATCAGCTCACCGGA
  AGGAAGCCTGTTCCTCACCCAAGGCCAGTGCCAAACTGGCGCTTATGAAAGCAAAAGAAGAAAGTTCTTA
  CAACGAAACAGAGCTGCTGGCTGCAAGAAGAAAAGAAAGCGCCACTGAACCCAAAGGAGAGAAAACAACT
  CCTAAGAAAACGAAAGTTTCTCCAACTAAGAGAGAGTCTGTAAGCCCAGAAGACTCTGAAAAGAAGCGCA
  CTAATTATCAAGCTTATCGAAGCTACTTGAACCGAGAAGGTCCCAAAGCTCTGGGCTCCAAAGAAATACC
  CAAGGGAGCTGAAAATTGCTTGGAAGGCCTGACGTTTGTGATCACAGGAGTGCTGGAGTCCATTGAACGA
  GACGAAGCCAAGTCTCTAATTGAACGTTATGGGGGGAAAGTAACAGGAAACGTGAGCAAGAAAACCAACT
  ACCTCGTCATGGGCCGCGACAGTGGGCAGTCCAAGAGTGACAAGGCAGCAGCTCTGGGAACAAAAATCCT
  CGATGAAGACGGCCTGTTGGATCTGATTCGAACTATGCCAGGCAAGAGATCCAAGTATGAGATGGCTGCT
  GAGGCTGAGATGAAGAAAGAAAAGTCCAAATTGGAGCGAACACCCCAAAAAAATGATCAAGGAAAAAGAA
  AAATTAGTCCAGCCAAGAAGGAGTCAGAGTCTAAAAAGTGCAAGCTGACTCTCCTAAAGAACAGTCCTAT
  GAAGGCAGTGAAGAAGGAGGCAAGCACGTGCCCAAGGGGCCTGGATGTCAAGGAGACACATGGCAACCGC
  AGTAGCAACAAAGAGGAATGTCTGCTCTGGGTGGATAAGTACAAGCCCGCCTCACTCAAGAACATAATCG
  GACAGCAGGGTGACCAGAGCTGTGCCAACAAATTACTACGTTGGCTCAGAAACTGGCACAAGAGTTCTCC
  TGAAGAGAAAAAACATGCAGCAAAGTTTGGCAAACTTGCCAGCAAAGATGATGGCTCCAGTTTCAAGGCA
  GCTCTGCTGTCTGGCCCTCCAGGTGTTGGCAAAACCACTACAGCTTCTCTTGTGTGTCAGGAATTGGGCT
  ACAGTTATGTGGAACTAAATGCAAGTGATACTCGGAGTAAGAACAGTTTGAAAGCAGTTGTTGCTGAATC
  CTTGAACAACACCAGCATCAAAGGCTTTTATACAAGTGGAGCGGCTCCTTCCGTGAGTGCGAGGCACGCC
  CTCATCATGGATGAGGTCGATGGCATGGCAGGCAATGAAGACAGGGGTGGAATTCAGGAGCTAATAGGCC
  TGATAAAGCATACTAAAATTCCCATCATTTGTATGTGCAATGATAGAAACCACCCCAAAATTCGCTCATT
  GGTTCATTATTGTTTTGATCTTCGTTTCCAAAGACCTCGAGTTGAACAGATTAAGAGTGCAATGCTGTCT
  ATTGCATTTAAAGAGGGTTTAAAAATCCCCCCTCCAGCTATGAATGAAATAATTTTGGGAGCTAATCAAG
  ATGTCAGACAGGTTTTACACAATCTCAGTATGTGGTGTGCACAAAGTAAGGCCCTGACCTATGACCAAGC
  CAAGGCTGATTCTCAGAGGGCTAAGAAGGATATCAGACTGGGCCCATTTGATGTCACTCGGAAAGTGTTT
  GCAGCTGGAGAGGAGACTGCACACATGTCACTGATGGACAAGTCAGATCTCTTCTTCCATGACTATTCCA
  TAGCACCCCTCTTTGTCCAGGAGAACTACCTGCATGTAAAGCCTGTGGCTGCAGGAGGTGACATGAAAAA
  GCACTTGATGCTTTTAAGCCGGGCAGCAGATAGCATATGTGACGGTGACTTAGTGGACAATCAGATCCGG
  AGTAAGCAAAACTGGAGTCTTCTGCCCACCCAGGCCATCTATGCCAGTGTTCTTCCTGGAGAGTTGATGC
  GGGGCTACATGACTCAGTTTCCGAGCTTCCCGAGCTGGCTGGGGAAGCACTCGTCTACGGGCAAACATGA
  TCGGATTGTTCAGGACCTATCCTTGCACATGAGTCTTAGAACTTACTCCAGCAAAAGGACAGTGAACATG
  GATTACCTGTCACACATCAGAGACGCACTTGTACGACCCTTGACCTCACAAGGGGTAGAAGGGGCTCAGC
  ATGTCATCAAACTTATGGACACATACTATCTGATGAAAGAAGATTTTGAGAACATCATGGAAGTTAGCAG
  CTGGGGGGGCAAGCCCAGTGCCTTTTCCAAGCTGGATCCCAAGGTGAAAGCGGCCTTCACAAGAGCCTAC
  AATAAGGAGGCCCACCTGACCCCATACTCACTTCAGGTAGTAAAGACATCGAGGCTCAGCACGGGTCCAG
  CACTGGATTCTGAGTACAGTGAGGAGTTCCAGGAAGATGACACTCAGTCTGAGAAAGAGCAGGATGCAGT
  GGAGACTGACGCCATGATCAAGAAAAAGACAAGATCTTCAAAACCTTCAAAGTCAGAAAGAGAGAAGGAG
  TCCAAAAAAGGAAAAGGAAAGAATTGGAAGAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211679 representing NM_011258
  Red=Cloning site Green=Tags(s)
  
  MDIRKFFGVISSGKKPVNETVKNEKTKASEGTVKGKKGVKEAKVNNSGKEDASKPKQHSKKKRIINDSDS
  ESEETVQVKNAKKKSEKLSLSYKPGKVSQKDPVTYVSETDEDDDFVCKKAASKSKENGVSTNSYLGTSNV
  KKNEENVKTKNKPLSPIKLTPTSVLDYFGTESVQRSGKKMVTSKRKESSQNTEDSRLNDEAIAKQLQLDE
  DAELERQLHEDEEFARTLALLDEEPKIKKARKDSEEGEESFSSVQDDLSKAEKQKSPNKAELFSTARKTY
  SPAKHGKGRASEDAKQPCKSAHRKEACSSPKASAKLALMKAKEESSYNETELLAARRKESATEPKGEKTT
  PKKTKVSPTKRESVSPEDSEKKRTNYQAYRSYLNREGPKALGSKEIPKGAENCLEGLTFVITGVLESIER
  DEAKSLIERYGGKVTGNVSKKTNYLVMGRDSGQSKSDKAAALGTKILDEDGLLDLIRTMPGKRSKYEMAA
  EAEMKKEKSKLERTPQKNDQGKRKISPAKKESESKKCKLTLLKNSPMKAVKKEASTCPRGLDVKETHGNR
  SSNKEECLLWVDKYKPASLKNIIGQQGDQSCANKLLRWLRNWHKSSPEEKKHAAKFGKLASKDDGSSFKA
  ALLSGPPGVGKTTTASLVCQELGYSYVELNASDTRSKNSLKAVVAESLNNTSIKGFYTSGAAPSVSARHA
  LIMDEVDGMAGNEDRGGIQELIGLIKHTKIPIICMCNDRNHPKIRSLVHYCFDLRFQRPRVEQIKSAMLS
  IAFKEGLKIPPPAMNEIILGANQDVRQVLHNLSMWCAQSKALTYDQAKADSQRAKKDIRLGPFDVTRKVF
  AAGEETAHMSLMDKSDLFFHDYSIAPLFVQENYLHVKPVAAGGDMKKHLMLLSRAADSICDGDLVDNQIR
  SKQNWSLLPTQAIYASVLPGELMRGYMTQFPSFPSWLGKHSSTGKHDRIVQDLSLHMSLRTYSSKRTVNM
  DYLSHIRDALVRPLTSQGVEGAQHVIKLMDTYYLMKEDFENIMEVSSWGGKPSAFSKLDPKVKAAFTRAY
  NKEAHLTPYSLQVVKTSRLSTGPALDSEYSEEFQEDDTQSEKEQDAVETDAMIKKKTRSSKPSKSEREKE
  SKKGKGKNWKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011258
• ORF Size: 3393 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011258.2, NP_035388.2
• RefSeq Size: 4731 bp
• RefSeq ORF: 3396 bp
• Locus ID: 19687
• UniProt ID: P35601
• Cytogenetics: 5 33.61 cM
• Gene Summary: The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction.[UniProtKB/Swiss-Prot Function]