Trip12 (BC034113) Mouse Tagged ORF Clone

CAT#: MG211639

Trip12 (tGFP-tagged) - Mouse thyroid hormone receptor interactor 12 (cDNA clone MGC:31615 IMAGE:4507222)

Product Images

Circular map for MG211639

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trip12
• Synonyms: MGC31615
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211639 representing BC034113
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTATCCAGCCAAGACCTGAAGATAGTAGTTGGAGCTCTTCAGATGGCAGAAATCCTAATGCAAAAGT
  TACCTGATATTTTTAGTGTTTACTTCAGGAGAGAAGGTGTGATGCATCAAGTCAAACATTTAGCAGAATC
  AGAGTCTTTGTTGACAAGTCCCCCCAAGGCATGTACAAATGGGTCAGGATCCCTGGGATCTACAACACCA
  GCCAGCAGTGGGACTGCAACGGCAGCAACCAACGCCTCTGCTGATCTGGGGTCACCTAGCTTGCAGCATA
  GCAGAGATGATTCTTTGGATCTGAGCCCTCAAGGTCGATTAAGTGATGTTCTCAAGAGAAAACGACTGCC
  AAAACGAGGGCCAAGAAGGCCAAAATATTCCCCTCCAAGAGATGATGACAAAGTAGACAATCAAGCTAAA
  AGCCCCACCACTACTCAGTCACCTAAATCTTCCTTCCTGGCAAGCTTGAATCCAAAAACATGGGGAAGGT
  TAAGCGCACAGTCCAACAGCAACAATATCGAACCAGCACGAACTGCAGGAGTTAGTGGTCTTGCCAGGGC
  AGCCTCAAAAGACACCATATCCAATAATAGAGAAAAAATTAAAGGCTGGATTAAGGAGCAGGCACATAAA
  TTTGTTGAGCGTTATTTTAGTTCTGAGAATATGGATGGAAGCAACCCTGCCTTGAATGTCCTTCAGAGAC
  TTTGTGCTGCAACTGAACAACTCAACCTCCAGGTGGATGGTGGAGCTGAGTGCCTTGTAGAAATCCGTAG
  CATAGTCTCAGAGTCAGATGTTTCTTCATTTGAAATCCAACATAGTGGGTTTGTGAAACAACTGTTGCTG
  TATTTGACATCTAAGAATGAAAAGGATGCTGTCGGCAGAGAGATCCGGTTAAAACGTTTCCTTCATGTAT
  TTTTTTCTTCTCCACTTCCTGGAGAAGAGCCTGTTGGAAGAGTAGAACCAGTGGGCCATGCACCTTTGCT
  GGCATTGGTTCACAAGATGAACAACTGTCTCAGCCAAATGGAGCAATTTCCAGTCAAAGTACATGATTTC
  CCTAGTGGGAACGGAGCTGGAGGAAGCTTTTCTCTCAACAGAGGATCACAAGCTTTAAAATTTTTCAACA
  CACATCAGTTAAAATGTCAGTTACAAAGACACCCAGACTGTGCAAATGTGAAGCAGTGGAAAGGCGGGCC
  TGTCAAGATTGATCCCCTGGCTCTGGTGCAAGCTATTGAGAGATACCTTGTGGTTAGAGGATATGGAAGA
  GTAAGAGAAGATGATGAAGATAGTGATGATGATGGGTCAGATGAAGAAATAGATGAGTCTTTGGCTGCTC
  AATTCTTAAATTCGGGAAATGTAAGGCACAGGCTACAGTTTTATATTGGAGAACATTTACTACCATATAA
  CATGACCGTGTATCAGGCAGTGCGGCAATTCAGTGTACAGGCTGAAGATGAAAGAGAATCTACTGATGAT
  GAGAGCAATCCTCTGGGAAGAGCTGGAATTTGGACAAAGACTCATACAATATGGTACAAACCTGTGAGGG
  AGGATGAAGAAAGTACTAAAGATTGTGTTGGTGGCAAAAGGGGGAGAGCCCAAACAGCTCCAACAAAAAC
  TTCCCCCAGAAATGCAAAGAAGCATGATGAGTTATGGCATGATGGAGTGTGCCCGTCAGTAGCAAATCCT
  TTAGAAGTTTACCTCATTCCCACACCACCTGAAAATATCACCTTTGAAGACCCATCCTTAGATGTAATAC
  TTCTTTTAAGAGTTTTACATGCCATCAGTCGATATTGGTATTACTTGTATGATAATGCGATGTGCAAGGA
  GATTATTCCAACTAGTGAATTTATTAACAGTAAATTAACAGCAAAAGCAAATAGGCAGCTTCAAGACCCT
  CTAGTAATCATGACAGGAAACATCCCAACATGGCTCACTGAGCTAGGAAAAACCTGCCCATTTTTCTTTC
  CTTTTGATACCCGACAAATGCTTTTTTATGTAACTGCATTTGATCGGGACCGGGCAATGCAAAGATTACT
  TGATACCAACCCAGAAATCAACCAGTCTGATTCTCAAGACAGCAGAGTTGCGCCTAGATTGGATAGAAAA
  AAACGTACTGTGAACAGAGAGGAGCTACTGAAACAAGCTGAGTCTGTGATGCAGGACCTTGGCAGCTCTC
  GGGCCATGTTAGAAATCCAATATGAAAATGAGGTTGGTACAGGTCTGGGGCCAACACTGGAGTTTTATGC
  ACTTGTTTCTCAGGAACTACAGAGAGCTGACTTGTGTCTCTGGAGGGGTGAAGAAGTAACTCTTAGCAAT
  CCAAAAGGAAGCCAAGAAGGGACCAAGTATATTCAAAACCTCCAGGGCCTGTTTGCGCTTCCCTTTGGTA
  GGACAGCTAAGCCAGCTCACATCGCAAAGGTTAAGATGAAGTTTCGCTTCTTAGGAAAATTAATGGCTAA
  GGCTATCATGGATTTCAGATTGGTGGACCTTCCTCTTGGCCTGCCCTTTTATAAGTGGATGCTGCGGCAG
  GAAACTTCATTGACATCTCATGATTTATTTGACATTGATCCTGTTGTAGCCAGATCAGTATATCACCTAG
  AAGACATTGTCAGACAGAAGAAAAGACTGGAGCAGGATAAATCCCAGACCAAAGAGAGTCTACAATATGC
  ATTGGAAACTCTTACGATGAATGGCTGTTCAGTGGAAGATCTGGGATTGGATTTTACGCTGCCTGGGTTT
  CCCAACATTGAATTGAAGAAAGGAGGGAAGGATATTCCAGTTACTATCCACAATTTGGAAGAGTACCTGA
  GATTGGTGATATTCTGGGCACTAAATGAAGGTGTTTGTCGACAGTTTGACTCATTCAGAGATGGATTTGA
  ATCGGTCTTCCCGCTCTGCCATCTTCAGTACTTCTACCCAGAAGAGCTGGACCAGCTCCTGTGTGGCAGT
  AAAGCAGACACTTGGGATGCAAAGACATTGATGGAATGCTGCAGGCCTGATCATGGCTATACTCATGACA
  GTCGGGCTGTGAAGTTCTTGTTTGAGATTCTCAGTAGTTTTGATAATGAGCAGCAGAGGTTGTTTCTCCA
  GTTTGTGACTGGTAGCCCACGATTGCCAGTTGGAGGATTTCGGAGTTTAAATCCACCTCTGACAATTGTG
  CGGAAGACATTTGAATCAACAGAAAACCCAGATGACTTCTTACCATCAGTAATGACTTGTGTGAACTATC
  TTAAATTGCCGGACTACTCGAGCATTGACATAATGCGGGACAAACTCTTGATAGCAGCAAGAGAAGGCCA
  ACAGTCGTTCCATCTTTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211639 representing BC034113
  Red=Cloning site Green=Tags(s)
  
  MLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSLGSTTP
  ASSGTATAATNASADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAK
  SPTTTQSPKSSFLASLNPKTWGRLSAQSNSNNIEPARTAGVSGLARAASKDTISNNREKIKGWIKEQAHK
  FVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLL
  YLTSKNEKDAVGREIRLKRFLHVFFSSPLPGEEPVGRVEPVGHAPLLALVHKMNNCLSQMEQFPVKVHDF
  PSGNGAGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGR
  VREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSVQAEDERESTDD
  ESNPLGRAGIWTKTHTIWYKPVREDEESTKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVANP
  LEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDP
  LVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRK
  KRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLCLWRGEEVTLSN
  PKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQ
  ETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGF
  PNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVCRQFDSFRDGFESVFPLCHLQYFYPEELDQLLCGS
  KADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIV
  RKTFESTENPDDFLPSVMTCVNYLKLPDYSSIDIMRDKLLIAAREGQQSFHLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC034113
• ORF Size: 3311 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034113, AAH34113
• RefSeq Size: 3945 bp
• RefSeq ORF: 3311 bp
• Locus ID: 14897
• Cytogenetics: 1 C5
• Gene Summary: E3 ubiquitin-protein ligase involved in ubiquitin fusion degradation (UFD) pathway and regulation of DNA repair. Part of the ubiquitin fusion degradation (UFD) pathway, a process that mediates ubiquitination of protein at their N-terminus, regardless of the presence of lysine residues in target proteins. In normal cells, mediates ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A, a lysine-less tumor suppressor required for p53/TP53 activation under oncogenic stress. In cancer cells, however, isoform p19ARF/ARF and TRIP12 are located in different cell compartments, preventing isoform p19ARF/ARF ubiquitination and degradation. Does not mediate ubiquitination of isoform p16-INK4a of CDKN2A. Also catalyzes ubiquitination of NAE1 and SMARCE1, leading to their degradation. Ubiquitination and degradation of target proteins is regulated by interaction with proteins such as MYC, TRADD or SMARCC1, which disrupt the interaction between TRIP12 and target proteins. Acts as a key regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.[UniProtKB/Swiss-Prot Function]