4931406P16Rik (NM_172741) Mouse Tagged ORF Clone

CAT#: MG211561

4931406P16Rik (tGFP-tagged) - Mouse RIKEN cDNA 4931406P16 gene (4931406P16Rik)

Product Images

Circular map for MG211561

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 4931406P16Rik
• Synonyms: AA553327; mKIAA0355
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211561 representing NM_172741
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATTGCTGCAGTGCCCAGGACAATAAGATGGACTACAAGCGGCGCTTCCTGCTTGGCGGGTCCAAGC
  AGAAGGTGCAGCAGCACCAGCAGTACCCGATGCCTGAGCTGGGCCGAGCACTGAGCGCTCCCCTGGCATC
  CACCGCCCCCTCTGCCCCGCTGGGCAGTCTGACCGCTGCAGGCAGCTGCCACCATGCCATGCCCCACTCT
  ACTCCCATTGCTGACATCCAGCAGGGCATCTCTAAGTACCTGGATGCCCTCAATGTCTTTTGCCGTGCTA
  GTACGTTCCTCACAGATCTCTTCAGCACCGTGTTCAGGAACTCACACTACTCAAAGGCCGCCATGCAGCT
  CAAAGATGTGCAGGAACATGTAATGGAAGCAGCCAGCAGATTGACCTCAGCCATAAAGCCCGAGATTGCC
  AAGATGCTCATGGAACTCAGTGCTGGGGCTGCAAACTTCACAGATCAGAAGGAATTCAGTCTCCAGGACA
  TTGAGGTGTTGGGGCGATGTTTCTTGACAGTGGTGCAAGTCCATTTCCAGTTTTTGACCCACGCCTTGCA
  GAAAGTCCAGCCGGTGGCTCACTCTTGCTTTGCTGAGGTAGTTGTGCCAGAAAAAAAGAACAGCAGTCTG
  TCTGGCATGGGCCACACACCTGAACTGGAGGAAGCTGTGCGATCCTGGCGAGGGGCAGCTGAGGCGACAT
  CTAGATTGAGAGAGAGAGGCTGTGATGGATGCCTGGCAGGAATCGAAGTCCAACAACTCTTTTGTTCTCA
  AAGTGCAGCAATTCCTGAGCACCAGCTAAAAGAGCTAAACATAAAAATCGACAGTGCTTTGCAGGCTTAT
  AAAATAGCTTTGGAAAGCTTAGGCCACTGTGAATATGCAATGAAAGCTGGCTTCCATCTAAACCCAAAGG
  CCATTGAAGCAAGTTTGCAGGGCTGCTGCAGTGAGGCGGAAGCTCAGCAGACAGGGCGAAGGCAGACACC
  CCCACAGCCCATGCAGTGTGAGCTCCCCACGGTTCCTGTGCAGATAGGATCGCACTTCCTGAAGGGGGTC
  TCCTTTAATGAGTCGGCTGCCGACAATCTGAAGCTTAAGACGCATACCATGTTACAGCTCATCAAGGAAG
  CAGGCTGCTACAATGGAATCACACCTAGGGATGATCTTCCTGTGACTGAAGTCCTGAATCAGGTGTGCCC
  GTCCATGTGGCGGGGTGCCTGTAAGACTGCTGTGCAGCTGCTGTTCGGCCAGGCAGGGCTGGTGGTTGTT
  GACACAGCACAGATTGAAAATAAAGAAGCCTATGCACCCCAGATCAGTCTTGAAGGTTCCAGAATTGTGG
  TTCAAGTCCCATCCACATGGTGCTTAAAGGAAGATCCTGCTACCATGTCCCTGCTGCAGAGGAGCCTGGA
  TCCCGAGAAGACCTTAGGTCTGGTGGATGTGCTTTACACAGCCGTGCTAGACCTAAACCGCTGGAGAACA
  GGGAGGGAACAAGCTTTACCCTGTATACAGATCCAGCTGCAAAGGGATATCTGCGATTTTGGGAATCAGG
  CTGACCTGCCTTCTGGAAATGGAAGCAAATCCTCGGGACTGCAGAAGACTTTCTCCAAACTGACATCCCG
  GTTCACAAAGAAAGTGTCATGCACCAACTCTGGCAGCACACACTATTCCAAAAACATCTTCACAGCTGGG
  TGTTCAGAGGAAAGGGCCAAAATGATGGGCAATAATATTGATACACGGTTACAGAGCATTTTGAACATTG
  GTAACTTCCCTAGGACTACAGACCCTTCACAGTCGTCTCAGAATTCCAGTAATCCAATGGTCAATGGCTT
  TCTCCTGGAGAGACGTGAGAACTTCCTGCATGGGGATGATGGCAAGGATGAGAAGGGCATGAACTTACCC
  ACTGATCAGGAAATGCAGGAAGTGATAGATTTTCTCTCAGGCTTTAACATGGGCCAGTCCCATCAGGGGT
  CCCCACTGGTGACAAGGCGTAACTCTACTGCCACTGCCATGGTGACTGAACAGAAGACAGGAACCATGCA
  ACCACAGCAGCCATCACTGCCATTGGCTCCTCCACTACGGCCACCCCAGGCTGGAGCACACACGCCTCTG
  GCAGCCCAGCAAGGTCTGGCTCCTCAGCAGCAGTCTCCAAAGCAGCAGCAGCCCCAAGTCCAGTACTACC
  AGCACCTGCTGCAACCCATTGGATCACAGCAGACTCCACCTCAGCCTCGGGCGCCTGCGAAATGGGTACA
  TGGCTCATCTCAGCACCCATCACAGCCCATGGCAGCGGGTCTGTCTCCTCTTGGTCAGTGGCCTGGCATA
  TCTGATCTCAGCTCTGACTTGTACAGCTTGGGTCTAGTGAGCAGCTATATGGACAATATGATGTCAGAAG
  TTTTGGGACAGAAGCCCCAGGGACCTAGAAATAACACCTGGCCAAACCGTGACCAAAGTGATGGAGTCTT
  TGGAATGCTGGGAGAGATTTTGCCTTTTGATCCTGCAGTGGGCTCAGACCCAGAATTCGCACGCTATGTG
  GCAGGAGTGAGCCAGGCAATGCAGCAGAAGCGGCAAGCTCAACATGGCCGCCGGCCAGGCAACCCCCGTG
  GCAACTGGCCGCCCATGGAAGACGCTCATCGGACCTGGCCTTTGCCAGAGTTCTTCACAGAAGGGGACAG
  CTTGCACAGCGGCTGGTCAGGAGCTCAGGGAGACTCGGCCAGTTCCAGTGATGAGACCTCTTCAGCCAAT
  GGGGACAGTCTGTTCTCCATGTTTTCAGGGCCTGACCTTGTTGCTGCTGTTAAACAGAGAAGGAAACACA
  GCAGTGGAGAGCAGGAGACCAGCACACTGCCCTCACCACCTCTCCTGACCACAGTGGAGGACGTGAACCA
  GGATAACAAAACCAAAACGTGGCCACCCAAAGCACCATGGCAGCACCCTTCCCCGATGCCCAGCACGCTG
  CCCAGCCCAGCTGCACCACTCTATGCAGTCGCCAGTCCTGGCAGCCAGTGGAACAACACCGTGCAGATGC
  TGCAGTCCCCTGTGTGGGCTGCAGCCAATGACTGCAATACCACCTCCTTCACCTATGTTCAGACACCACC
  ACAGCCCCCACCTCCACCAGCACATAAGGCAGCACCCAAGGGCTTCAAGGCCTTCCCTGGGAAGGCTGAA
  CGTAGGCCAGCCTATTTGCCCCAGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211561 representing NM_172741
  Red=Cloning site Green=Tags(s)
  
  MYCCSAQDNKMDYKRRFLLGGSKQKVQQHQQYPMPELGRALSAPLASTAPSAPLGSLTAAGSCHHAMPHS
  TPIADIQQGISKYLDALNVFCRASTFLTDLFSTVFRNSHYSKAAMQLKDVQEHVMEAASRLTSAIKPEIA
  KMLMELSAGAANFTDQKEFSLQDIEVLGRCFLTVVQVHFQFLTHALQKVQPVAHSCFAEVVVPEKKNSSL
  SGMGHTPELEEAVRSWRGAAEATSRLRERGCDGCLAGIEVQQLFCSQSAAIPEHQLKELNIKIDSALQAY
  KIALESLGHCEYAMKAGFHLNPKAIEASLQGCCSEAEAQQTGRRQTPPQPMQCELPTVPVQIGSHFLKGV
  SFNESAADNLKLKTHTMLQLIKEAGCYNGITPRDDLPVTEVLNQVCPSMWRGACKTAVQLLFGQAGLVVV
  DTAQIENKEAYAPQISLEGSRIVVQVPSTWCLKEDPATMSLLQRSLDPEKTLGLVDVLYTAVLDLNRWRT
  GREQALPCIQIQLQRDICDFGNQADLPSGNGSKSSGLQKTFSKLTSRFTKKVSCTNSGSTHYSKNIFTAG
  CSEERAKMMGNNIDTRLQSILNIGNFPRTTDPSQSSQNSSNPMVNGFLLERRENFLHGDDGKDEKGMNLP
  TDQEMQEVIDFLSGFNMGQSHQGSPLVTRRNSTATAMVTEQKTGTMQPQQPSLPLAPPLRPPQAGAHTPL
  AAQQGLAPQQQSPKQQQPQVQYYQHLLQPIGSQQTPPQPRAPAKWVHGSSQHPSQPMAAGLSPLGQWPGI
  SDLSSDLYSLGLVSSYMDNMMSEVLGQKPQGPRNNTWPNRDQSDGVFGMLGEILPFDPAVGSDPEFARYV
  AGVSQAMQQKRQAQHGRRPGNPRGNWPPMEDAHRTWPLPEFFTEGDSLHSGWSGAQGDSASSSDETSSAN
  GDSLFSMFSGPDLVAAVKQRRKHSSGEQETSTLPSPPLLTTVEDVNQDNKTKTWPPKAPWQHPSPMPSTL
  PSPAAPLYAVASPGSQWNNTVQMLQSPVWAAANDCNTTSFTYVQTPPQPPPPPAHKAAPKGFKAFPGKAE
  RRPAYLPQY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172741
• ORF Size: 3177 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172741.2, NP_766329.1
• RefSeq Size: 5808 bp
• RefSeq ORF: 3180 bp
• Locus ID: 233103
• Cytogenetics: 7 B1