Col6a2 (NM_146007) Mouse Tagged ORF Clone

CAT#: MG211493

Col6a2 (tGFP-tagged) - Mouse procollagen, type VI, alpha 2 (Col6a2)

Product Images

Circular map for MG211493

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Col6a2
• Synonyms: Col6a-2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211493 representing NM_146007
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTACTATCAAGATGCTCCAGGGTCCTCTTTCTGTGCTCCTGATTGGGGGACTCTTGGGGGTCCTCC
  ATGCCCAGCAGCAGGAAGCCATCTCACCCCAGGAGCAGGAAGCTGTCTCACCAGACATCTCCACCACTGA
  AAGGAACAACAATTGTCCAGAGAAGGCCGACTGCCCAGTCAACGTGTATTTCGTGTTGGACACCTCAGAG
  AGCGTGGCCATGCAGTCCCCGACAGACAGCCTGCTCTATCATATGCAGCAGTTCGTACCGCAGTTTATCA
  GCCAGCTGCAGAACGAGTTCTACCTGGACCAGGTGGCCCTGAGCTGGCGCTACGGTGGTCTACACTTCTC
  GGACCAAGTGGAGGTGTTCAGCCCACCGGGCAGTGACCGGGCCTCCTTCACTAAGAGCCTACAAGGCATC
  CGCTCCTTCCGCAGGGGCACCTTCACTGACTGTGCATTGGCTAACATGACGCAGCAGATCCGGCAGCACG
  TAGGCAAGGGGGTGGTCAACTTCGCCGTGGTCATCACTGACGGCCACGTCACGGGCAGTCCGTGTGGGGG
  CATCAAGATGCAGGCTGAGCGTGCCCGTGAAGAGGGCATCCGGCTCTTCGCTGTGGCCCCTAACAGGAAC
  CTAAACGAACAAGGCCTGAGGGACATCGCTAACTCTCCACATGAGCTCTACCGTAACAACTACGCCACCA
  TGCGACCCGACTCTACCGAGATTGACCAGGACACCATCAACCGCATCATCAAGGTCATGAAACATGAAGC
  CTATGGAGAGTGCTACAAGGTGAGCTGCCTGGAGATTCCTGGACCCCACGGACCCAAGGGTTACCGAGGA
  CAGAAGGGTGCCAAGGGCAACATGGGTGAACCAGGAGAGCCTGGACAGAAAGGACGACAGGGAGACCCCG
  GCATCGAAGGCCCCATTGGATTCCCGGGACCGAAGGGTGTGCCTGGCTTCAAGGGAGAGAAGGGTGAATT
  TGGATCGGATGGTCGGAAGGGAGCGCCTGGCCTAGCTGGCAAGAATGGAACAGATGGACAGAAGGGCAAA
  CTGGGCCGCATTGGGCCTCCTGGTTGCAAGGGAGACCCCGGAAGTCGGGGCCCCGATGGATACCCTGGAG
  AAGCTGGAAGCCCAGGCGAGCGAGGAGACCAGGGTGCCAAGGGGGACTCTGGCCGCCCAGGACGCAGGGG
  ACCACCAGGAGATCCTGGAGACAAAGGAAGCAAGGGATATCAAGGCAACAACGGAGCCCCTGGAAGCCCG
  GGAGTGAAAGGAGGCAAGGGAGGGCCTGGCCCCCGTGGACCAAAAGGAGAGCCTGGACGCAGAGGAGACC
  CCGGGACCAAGGGCGGCCCCGGCAGCGATGGTCCAAAGGGAGAGAAGGGAGACCCTGGTCCTGAGGGGCC
  TCGAGGCCTGGCTGGAGAAGTTGGCAGTAAAGGAGCCAAGGGAGACAGAGGTTTGCCTGGACCCAGAGGC
  CCCCAGGGGGCTCTTGGAGAGCCAGGAAAGCAGGGATCTCGAGGAGACCCTGGTGACGCCGGACCTCGAG
  GGGATTCAGGACAGCCGGGCCCCAAGGGCGATCCTGGAAGGCCTGGATTCAGCTACCCGGGACCTCGAGG
  GACACCCGGTGAAAAAGGCGAGCCCGGTCCACCAGGCCCTGAGGGAGGCCGAGGAGACTTTGGTCTGAAA
  GGAACACCCGGACGGAAGGGAGATAAAGGGGAGCCAGCTGATCCTGGTCCCCCTGGTGAACCTGGCCCTC
  GGGGGCCAAGAGGAATCCCAGGACCTGAGGGAGAACCCGGCCCTCCAGGAGACCCTGGTCTCACGGAATG
  TGATGTCATGACCTATGTGAGGGAGACCTGTGGATGCTGCGACTGTGAGAAGCGCTGTGGTGCCCTGGAT
  GTGGTCTTCGTCATCGACAGTTCTGAGAGTATTGGCTACACCAACTTCACCTTGGAGAAGAACTTTGTCA
  TCAATGTGGTCAACAGGCTAGGTGCCATTGCCAAGGACCCCAAGTCAGAAACAGGCACACGTGTGGGTGT
  GGTGCAGTACAGCCACGAGGGCACCTTTGAGGCCATCCGGCTGGACGACGAGCGAGTCAACTCCCTGTCT
  AGTTTCAAGGAGGCTGTCAAAAACCTTGAATGGATCGCCGGTGGCACTTGGACGCCCTCTGCCCTCAAGT
  TTGCCTATAATCAGCTCATCAAAGAAAGCCGGCGCCAGAAGACCCGGGTGTTCGCAGTGGTCATCACGGA
  TGGGCGCCATGACCCCCGAGATGATGACCTCAATCTTCGGGCACTGTGTGACCGAGATGTCACTGTGACA
  GCCATTGGCATCGGTGACATGTTCCACGAGACTCATGAGAGTGAGAACCTCTACTCCATTGCCTGTGACA
  AGCCACAGCAAGTGCGCAACATGACGCTGTTCTCTGACCTGGTGGCCGAGAAGTTCATCGATGACATGGA
  AGACGTCCTTTGTCCAGACCCCCAGATCGTGTGTCCAGAACTTCCCTGCCAAACAGAGCTCTATGTGGCC
  CAGTGCACACAACGGCCCGTGGACATTGTCTTCCTGCTGGATGGCTCGGAGCGGCTGGGCGAGCAGAACT
  TCCACAAGGTGCGGCGCTTCGTGGAGGACGTGTCCCGGCGCCTGACTCTGGCCCGGAGGGATGATGACCC
  ACTCAACGCCCGCATGGCTCTGTTGCAATATGGCAGCCAGAATCAGCAACAGGTGGCCTTCCCACTGACC
  TACAACGTGACCACCATCCACGAGGCCCTGGAGAGGGCCACCTACCTCAATTCCTTTTCTCACGTGGGCA
  CGGGCATCGTACACGCCATCAACAACGTGGTGCGGGGGGCACGGGGTGGGGCGCGGCGCCACGCAGAGCT
  CTCCTTCGTCTTCCTCACGGACGGTGTCACCGGCAATGACAGCCTGGAGGAGTCAGTGCACTCTATGCGT
  AAGCAGAACGTGGTGCCCACTGTGGTCGCTGTGGGCGGCGACGTGGACATGGATGTGCTTACTAAGATCA
  GCCTGGGTGACAGGGCGGCCATCTTCCGGGAGAAAGACTTTGACAGTCTGGCCCAGCCCAGCTTCTTTGA
  CAGGTTCATCCGCTGGATCTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211493 representing NM_146007
  Red=Cloning site Green=Tags(s)
  
  MTTIKMLQGPLSVLLIGGLLGVLHAQQQEAISPQEQEAVSPDISTTERNNNCPEKADCPVNVYFVLDTSE
  SVAMQSPTDSLLYHMQQFVPQFISQLQNEFYLDQVALSWRYGGLHFSDQVEVFSPPGSDRASFTKSLQGI
  RSFRRGTFTDCALANMTQQIRQHVGKGVVNFAVVITDGHVTGSPCGGIKMQAERAREEGIRLFAVAPNRN
  LNEQGLRDIANSPHELYRNNYATMRPDSTEIDQDTINRIIKVMKHEAYGECYKVSCLEIPGPHGPKGYRG
  QKGAKGNMGEPGEPGQKGRQGDPGIEGPIGFPGPKGVPGFKGEKGEFGSDGRKGAPGLAGKNGTDGQKGK
  LGRIGPPGCKGDPGSRGPDGYPGEAGSPGERGDQGAKGDSGRPGRRGPPGDPGDKGSKGYQGNNGAPGSP
  GVKGGKGGPGPRGPKGEPGRRGDPGTKGGPGSDGPKGEKGDPGPEGPRGLAGEVGSKGAKGDRGLPGPRG
  PQGALGEPGKQGSRGDPGDAGPRGDSGQPGPKGDPGRPGFSYPGPRGTPGEKGEPGPPGPEGGRGDFGLK
  GTPGRKGDKGEPADPGPPGEPGPRGPRGIPGPEGEPGPPGDPGLTECDVMTYVRETCGCCDCEKRCGALD
  VVFVIDSSESIGYTNFTLEKNFVINVVNRLGAIAKDPKSETGTRVGVVQYSHEGTFEAIRLDDERVNSLS
  SFKEAVKNLEWIAGGTWTPSALKFAYNQLIKESRRQKTRVFAVVITDGRHDPRDDDLNLRALCDRDVTVT
  AIGIGDMFHETHESENLYSIACDKPQQVRNMTLFSDLVAEKFIDDMEDVLCPDPQIVCPELPCQTELYVA
  QCTQRPVDIVFLLDGSERLGEQNFHKVRRFVEDVSRRLTLARRDDDPLNARMALLQYGSQNQQQVAFPLT
  YNVTTIHEALERATYLNSFSHVGTGIVHAINNVVRGARGGARRHAELSFVFLTDGVTGNDSLEESVHSMR
  KQNVVPTVVAVGGDVDMDVLTKISLGDRAAIFREKDFDSLAQPSFFDRFIRWIC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_146007
• ORF Size: 3102 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146007.2
• RefSeq Size: 3739 bp
• RefSeq ORF: 3105 bp
• Locus ID: 12834
• UniProt ID: Q02788
• Cytogenetics: 10 39.32 cM
• Gene Summary: Collagen VI acts as a cell-binding protein.[UniProtKB/Swiss-Prot Function]