Kdm4a (NM_172382) Mouse Tagged ORF Clone

CAT#: MG211491

Kdm4a (tGFP-tagged) - Mouse jumonji domain containing 2A (Jmjd2a)

Product Images

Circular map for MG211491

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kdm4a
• Synonyms: D4Ertd222e; JHDM3A; Jmjd2; Jmjd2a; mKIAA0677
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211491 representing NM_172382
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCTGAATCAGAAACTTTGAACCCCAGTGCTCGGATCATGACCTTTTACCCCACCATGGAAGAGT
  TCCGGAACTTCAGCCGCTACATTGCCTACATTGAATCCCAAGGAGCTCATCGGGCTGGGCTAGCCAAGGT
  CGTTCCTCCCAAAGAGTGGAAGCCTCGAACATCCTACGACGATATTGATGATCTAGTCATCCCTGCGCCC
  ATCCAGCAGCTGGTGACTGGCCAGTCTGGCCTCTTCACTCAGTACAACATACAGAAGAAAGCCATGACCG
  TTCGTGAGTTCCGCAAGATCGCCAATAGCGACAAGTACTGCACCCCGCGATACAGTGAATTTGAAGAACT
  TGAGCGTAAATACTGGAAAAACCTCACATTCAATCCTCCCATCTATGGAGCAGACGTGAATGGTACTCTC
  TATGAACAGCACGTTGATGAGTGGAATATCGGCCGGCTGAAGACCATCCTGGACCTGGTGGAGAAGGAGA
  GCGGGATCACCATTGAGGGGGTGAACACCCCCTACCTTTACTTCGGCATGTGGAAGACGTCCTTCGCCTG
  GCACACGGAAGACATGGATCTATACAGCATCAACTATCTGCACTTCGGAGAGCCAAAGTCTTGGTACTCT
  GTTCCACCTGAGCATGGGAAACGCCTGGAGCGTCTTGCCAAAGGCTTTTTCCCAGGAAGTGCTCAAAGCT
  GTGAGGCTTTTCTCCGCCACAAGATGACCCTGATCTCCCCATTAATGCTGAAGAAGTATGGCATTCCCTT
  TGACAAGGTGACCCAAGAAGCTGGCGAGTTTATGATCACTTTTCCGTATGGTTACCATGCTGGCTTCAAC
  CATGGCTTCAACTGTGCAGAGTCAACCAATTTTGCTACCCGTCGGTGGATTGAGTATGGCAAGCAGGCTG
  TGCTGTGCTCCTGCAGAAAAGATATGGTGAAGATCTCCATGGACGTGTTCGTGAGGAGGTTCCAGCCGGA
  ACGCTACAAACTTTGGAAAGCTGGGAAGGACAGCATGGTTATTGACCACACTCTGCCCACACCGGAAGCA
  GCCGAGTTCCTGAAGGACAGCGGCGGACTAACCCCGAGAGCAGGGAGTGAGGAGTGCCCAGAGGAGGACG
  TGGAAGCAGCGGATCAGGGAGAGGAGGGGGATGTGAAGAGAAGCCTGGCCAAGCATCGGATCGGGACAAA
  GAGACACAGAGTCTGTCTGGAAATACCCCAGGAGGTCAGTCAGAGCGAGCTCTTCCCCAAGGAAGAGCTA
  AGTTCTGGACAGTATGAGATGACAGAGTGCCCGGCCACCCTCGCCCCAGTGAGGCCCACCCACAGCTCCG
  TGCGGCAAGTTGAGGACAGTCTTCCCTTCCCAGATTACTCTGACCCCACTGAAGTCAAATTTGAAGAGCT
  GAAGAATGTCAAACTAGAAGAGGAAGATGAGGAGGATGAGCCCGAAGCAGCCGCACTGGACCTTTCTGTG
  AATCCTGCGTCTGTAGGAGGACGCCTCGTCTTCTCGGGTTCCAAAAAGAAATCATCTTCCAGCCTGGGCT
  CCACTTCATCTCAGGATTCAGTTTCTTCAGATTCTGAAACCGCCGAGTCTGTCTCTTGCCAGGGCCAAGA
  GAAAACGGGAGTTCTCACCGTGCACAGCTATGCCAGAGGGGACGGCAAGGCTGCCACAGGAGAGCCGAGT
  GTGAAGAAGAAGCGCAGTGCTCCCCGCAGCATCAGCGAGCAGGAGCTGGCAGAGGTTGCAGATGAGTACA
  TGCTTTCCCTGGAGGAGAATAAGAAGACCAAGGGTCGCCGCCAGCCCTTGAGCAAGCTCCCACGCCACCA
  CCCACTCGTGCTGCAGGAGTGCGGCAGCGATGACGAGACTTCTGAGCAGCTGACCCCCGAGGAGGAGGCT
  GAGGAGACAGAGGCTTGGGCCAAGCCTCTGAGCCAGCTGTGGCAGAACCGACCCCCGAACTTTGAGGCTG
  AAAAGGAATTCAATGAGATCATGGCCCAACAGGCCCCTCACTGTGCTGTGTGTATGATCTTCCAGACCTA
  CCATCAGGTGGAATTTGGAGCCTTCAGTCAGAGCTGTGGGGATGCTTCAGAGCCGGCTGCCCAGACTCAG
  AGGACCAAGCCACTGATCCCCGAAATGTGCTTTACCACCACTGGCTGCAGCACAGACATCAATCTGTCCA
  CCCCGTACCTAGAGGAGGATGGCACCAGCATGCTAGTGTCCTGCAAGAAGTGCAGTGTCCGGGTCCACGC
  CAGTTGCTATGGCGTCCCTCCTGCCAAGGCTTCTGAAGAATGGATGTGCTCTCGATGTTCAGCCAATGCC
  CTGGAGGAAGACTGCTGCTTATGCTCGTTGCGTGGAGGGGCCCTGCAGAGAGCCAATGACGACAGATGGG
  TTCATGTTTCATGTGCAGTGGCAATTCTAGAAGCAAGGTTTGTCAACATTGCTGAAAGAAGTCCAGTGGA
  TGTGAGCAAAATCCCTCTGCCCCGCTTCAAACTGAAGTGTGTTTTTTGTAAGAAGCGGAGAAAACGGAAT
  GCTGGCTGCTGTGTGCAGTGCTCCCATGGCCGCTGCCCCACTGCCTTCCATGTGAGCTGTGCCCAGGCTG
  CTGGGGTGATGATGCAACCCGACGACTGGCCCTTTGTTGTCTTCATCACGTGCTTCCGTCACAAGATTCC
  CAATCTGGAGCGTGCTAAGGGAGCCTTGCTGAGCATCACGGCGGGCCAGAAGGTTATCAGCAAACACAAG
  AACGGGCGCTTCTACCAGTGTGAGGTGGTCAGACTCACCACCGAGACCTTCTACGAAGTCAACTTTGACG
  ACGGCTCCTTCAGTGACAACCTTTATCCTGAGGACATAGTGGTAATGTCTGAAAGCGGCTTCTGGCAGCA
  CTTTGGGTCTGGGACATCTTCTTGTTATTGCCGGCTGGATGACTGTGGTCTCTTTGCCTGCCCCTGGTCA
  GTTTCCAAACAGAAAGAGCCTTTATTCCCAGGTTCCCTTTCAAGGAAGAGTGGACATGCTGGGGCGCTCA
  GCTTCCCCGAGGAGTTCAGAGGAGTCAGTGTTCCCTGCAGTCCACTGAAATATGCTTATATCTCAGACCA
  AATTATTTCTAATAGCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211491 representing NM_172382
  Red=Cloning site Green=Tags(s)
  
  MASESETLNPSARIMTFYPTMEEFRNFSRYIAYIESQGAHRAGLAKVVPPKEWKPRTSYDDIDDLVIPAP
  IQQLVTGQSGLFTQYNIQKKAMTVREFRKIANSDKYCTPRYSEFEELERKYWKNLTFNPPIYGADVNGTL
  YEQHVDEWNIGRLKTILDLVEKESGITIEGVNTPYLYFGMWKTSFAWHTEDMDLYSINYLHFGEPKSWYS
  VPPEHGKRLERLAKGFFPGSAQSCEAFLRHKMTLISPLMLKKYGIPFDKVTQEAGEFMITFPYGYHAGFN
  HGFNCAESTNFATRRWIEYGKQAVLCSCRKDMVKISMDVFVRRFQPERYKLWKAGKDSMVIDHTLPTPEA
  AEFLKDSGGLTPRAGSEECPEEDVEAADQGEEGDVKRSLAKHRIGTKRHRVCLEIPQEVSQSELFPKEEL
  SSGQYEMTECPATLAPVRPTHSSVRQVEDSLPFPDYSDPTEVKFEELKNVKLEEEDEEDEPEAAALDLSV
  NPASVGGRLVFSGSKKKSSSSLGSTSSQDSVSSDSETAESVSCQGQEKTGVLTVHSYARGDGKAATGEPS
  VKKKRSAPRSISEQELAEVADEYMLSLEENKKTKGRRQPLSKLPRHHPLVLQECGSDDETSEQLTPEEEA
  EETEAWAKPLSQLWQNRPPNFEAEKEFNEIMAQQAPHCAVCMIFQTYHQVEFGAFSQSCGDASEPAAQTQ
  RTKPLIPEMCFTTTGCSTDINLSTPYLEEDGTSMLVSCKKCSVRVHASCYGVPPAKASEEWMCSRCSANA
  LEEDCCLCSLRGGALQRANDDRWVHVSCAVAILEARFVNIAERSPVDVSKIPLPRFKLKCVFCKKRRKRN
  AGCCVQCSHGRCPTAFHVSCAQAAGVMMQPDDWPFVVFITCFRHKIPNLERAKGALLSITAGQKVISKHK
  NGRFYQCEVVRLTTETFYEVNFDDGSFSDNLYPEDIVVMSESGFWQHFGSGTSSCYCRLDDCGLFACPWS
  VSKQKEPLFPGSLSRKSGHAGALSFPEEFRGVSVPCSPLKYAYISDQIISNSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172382
• ORF Size: 3099 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172382.2, NP_759014.2
• RefSeq Size: 3376 bp
• RefSeq ORF: 3102 bp
• Locus ID: 230674
• UniProt ID: Q8BW72
• Cytogenetics: 4 54.31 cM
• Gene Summary: Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:24953653). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively (By similarity).[UniProtKB/Swiss-Prot Function]